Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production

Fuentes-Grünewald, Claudio; Bayliss, Catherine E.; Zanain, M.; Pooley, Craig L.; Scolamacchia, M.; Silkina, Alla

doi:10.1016/j.biortech.2015.04.042

Cited by 56 publications

(37 citation statements)

References 31 publications

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“…Porphyridium purpureum has the potential to fill a number of niche areas within these industrial sectors by possessing the ability to produce a broad range of commercially valuable chemicals such as phycobiliproteins (PBP) (Guihéneuf & Stengel, 2015), sulphated exopolysaccharides (EPS) (Fuentes-Grünewald et al, 2015), and polyunsaturated fatty acids (PUFAs) (Durmaz et al, 2007). Phycobiliproteins are a group of coloured water-soluble, αβ heterodimeric proteins that constitute the major complex light-harvesting pigments of cyanobacteria, red algae, glaucocystophytes, and cryptophytes (Bermejo Román et al, 2002;Roy et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum

Coward

Fuentes-Grünewald

Silkina

et al. 2016

Bioresource Technology

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The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied. Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest. The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly. However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (∼2.9% DW), zeaxanthin (∼586μggDW), β-carotene (397μggDW), exopolysaccharides (2.05g/L), and phycobiliproteins (∼4.8% DW). This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed.

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Section: Introductionmentioning

confidence: 99%

“…Due to the wide range of applications of PBPs, the total market value is estimated to be >US$ 60 million (Borowitzka, 2013). EPS are complex sulphated polysaccharides composed of different sugar monomers such as xylose, glucose, and galactose (Fuentes-Grünewald et al, 2015;Patel et al, 2013;Sun et al, 2008). Porphyridium spp.…”

Section: Introductionmentioning

confidence: 99%

Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum

Coward

Fuentes-Grünewald

Silkina

et al. 2016

Bioresource Technology

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“…where: µ is the specific growth rate, T d is the doubling time Dry weight was measured according to [31], a known volume of algae was pelleted and washed with deionized (DI) water (three times using 25 mL of DI water each time) prior to being filtered onto a preweighed and dried filters (Whatman GF/F 47 mm Ø). The filters with algal biomass were then dried and reweighed until constant weight was reached.…”

Section: Sampling and Analysismentioning

confidence: 99%

Large-Scale Waste Bio-Remediation Using Microalgae Cultivation as a Platform

et al. 2019

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Municipal and agricultural waste treatment is one of the key elements of reducing environmental impact with direct effects on the economy and society. Algal technology has been tested to enable effective recycling and valorisation of wastewater nutrients including carbon, nitrogen and phosphorus. An integrated evaluation and optimisation of the sustainability of an algal bio-refinery, including mass and energy balances, carbon, water and nutrient use and impact analysis, was assessed. A bio-refinery approach of waste remediation using algal cultivation was developed at Swansea University, focusing on nutrient recovery via algal biomass exploitation in pilot facilities. Mass cultivation (up to 1.5 m3) was developed with 99% of nitrogen and phosphorus uptake by microalgal cultures. Nannochloropsis oceanica was used as a biological model and grown on three waste sources. The compounds obtained from the biomass were evaluated for animal feed and as a potential source of energy. The bioremediation through algal biotechnology was examined and compared to alternative nutrient recovery passive and active methods in order to know the most efficient way of excess nutrient management. Conclusions emphasise the high potential of algal biotechnology for waste remediation and nutrients recovery, despite the need for further development and scalable applications of this new technology.

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“…Furthermore, considerable amounts of research have been focused on the development of photobioreactors (PBRs) over the past two decades and a wide range of different designs and configurations have been built and tested around the world (Pulz 2001; Singh and Sharma 2012). Successful PBR cultivation systems, with high biomass productivity, have been demonstrated in high latitudes (Fuentes-Gruenewald et al 2015). These have used semi-continuous cultivation approaches with resultant high nutrient removal rates employing Porphyridium purpureum and Scenedsmus sp.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation efficacy—comparison of nutrient removal from an anaerobic digest waste-based medium by an algal consortium before and after cryopreservation

et al. 2017

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An algal consortium was isolated from an integrated steelmaking site at TATA Steel Strip Products Ltd. in Port Talbot, UK, and its bioremediation capacity tested. Excellent “bioremediation” was observed when the mixed culture was “applied” to diluted effluent from an enhanced anaerobic digestion plant at Dŵr Cymru Welsh Water at Port Talbot, UK. After 5 days of cultivation in a 600-L photobioreactor, 99% of the total nitrogen (initial level, 4500 μmol L−1) and total phosphorus (initial level, 690.4 μmol L−1) were removed from the waste stream. The consortium was deposited in the Culture Collection of Algae and Protozoa (CCAP), an international depository authority for microalgal patents, as CCAP 293/1. This material has been successfully cryopreserved using a two-step cryopreservation protocol with dimethyl sulphoxide (5% v/v) used as a cryoprotectant. On recovery of samples after 3 months storage at −196 °C, the specific bioremediation activity of the revived consortium was tested. The capacity of the revived culture to bioremediate effluent was not significantly different (p < 0.05) from a non-cryopreserved control, with 99% of total nitrogen and phosphorus remediated by day 4. Although non-axenic algal cultures have previously been cryopreserved, this is the first report of the successful cryopreservation of mixed algal consortium, with validation of its ability to bioremediate after thawing comparing non-cryopreserved cultures with a revived post-thaw algal consortium. The study also highlights the need to ensure the long-term security and the requirement to validate the functionality of conserved inocula with biotechnological/bioremediation potential.

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Evaluation of batch and semi-continuous culture of Porphyridium purpureum in a photobioreactor in high latitudes using Fourier Transform Infrared spectroscopy for monitoring biomass composition and metabolites production

Cited by 56 publications

References 31 publications

Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum

Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum

Large-Scale Waste Bio-Remediation Using Microalgae Cultivation as a Platform

Bioremediation efficacy—comparison of nutrient removal from an anaerobic digest waste-based medium by an algal consortium before and after cryopreservation

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