Evaluation of Bar, Barnase, and Barstar recombinant proteins expressed in genetically engineered Brassica juncea (Indian mustard) for potential risks of food allergy using bioinformatics and literature searches

Vasanthi, S; Bharatraj, Dinesh Kumar; Vankudavath, Raju Naik; Mendu, Vishnu Vardhana Rao; Gupta, Vibha; Goodman, Richard E.

doi:10.1016/j.fct.2015.06.003

Cited by 17 publications

(6 citation statements)

References 35 publications

(38 reference statements)

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“…The AOL has been used by the sponsoring companies to evaluate potential risks of allergenicity of their GM proteins. It has also been used in studies to evaluate potential products developed by nonprofit organizations and academic developers for example, Indian mustard plants designed to enhance hybrid seed production .…”

Section: Discussionmentioning

confidence: 99%

AllergenOnline: A peer‐reviewed, curated allergen database to assess novel food proteins for potential cross‐reactivity

Goodman

Ebisawa

Ferreira

et al. 2016

Molecular Nutrition Food Res

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158

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Section: Discussionmentioning

confidence: 99%

AllergenOnline: A peer‐reviewed, curated allergen database to assess novel food proteins for potential cross‐reactivity

Goodman

Ebisawa

Ferreira

et al. 2016

Molecular Nutrition Food Res

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158

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“…Bioinformatic evaluations were then performed to investigate possible similarities with toxins or allergens using the NCBI Entrez Protein 8 database as well as the AllergenOnline.org database (Version 18), with methods previously described ( 14 , 15 ). BLASTP searches of the NCBI Entrez Protein database were done to compare any putative peptide sequences against all protein sequences to determine the prevalence of common homologs using keywords.…”

Section: Methodsmentioning

confidence: 99%

“…Abbreviations: AA, amino acid; ALA, α-linoleic acid, 18:3 9,12,15 ; CSIRO, Commonwealth Scientific and Industrial Research Organisation; DHA, docosahexaenoic acid, 22:6 4,7,10, 13,16,19 ; DHA canola, genetically engineered canola, event NS-B5ØØ27-4; DPA, docosapentaenoic acid, 22:5 7,10,13,16,19 ; DGLA, dihomo-γ-linolenic acid; DW, dry weight; EPA, eicosapentaenoic acid, 20:5 5,8,11,14,17 ; ETA, eicosatetraenoic acid, 20:4 8,11,14,17 ; ETE, eicosatrienoic acid, 20:3 11,14,17 ; FA, fatty acid(s); GLA, γ-linolenic acid; GRAS, generally recognized a safe; Ha, hectares; ILSI, International Life Sciences Institute; LA, linoleic acid, 18:2 9,12 ; LC-MRM-MS, liquid chromatography-multiple reaction monitoring-mass spectrometry; LC-MS, liquid chromatography-mass spectrometry; LCPUFA, long chain (≥C20) polyunsaturated fatty acids; LOD, limit of detection; LOQ, limit of quantification; Micpu-6D, Micromonas pusilla 6-desaturase; OA, oleic acid, 18:1 9 ; ORF, open reading frame; PAT, phosphinothricin-N-acetyltransferase; Pavsa-5D, Pavlova salina 5-desaturase; Picpa-ω3D, Pichia pastoris 15-/ω3-desaturase; Pyrco-5E, Pyramimonas cordata 5-elongase; SDA, stearidonic acid, 18:4 6,9,12,15 ; US FDA, United States Food and Drug Administration; ω3, omega-3; ω6, omega-6. 1 http://www.fao.org/faostat/en/?#data/QC Agricultural biotechnology has been widely adopted by growers demonstrating that the technology brings exceptional benefits for effective control of crop pests, reduced inputs, and increased cost savings.…”

Section: Introductionmentioning

confidence: 99%

Food and Feed Safety of NS-B5ØØ27-4 Omega-3 Canola (Brassica napus): A New Source of Long-Chain Omega-3 Fatty Acids

MacIntosh¹,

Shaw²,

Connelly³

et al. 2021

Front. Nutr.

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DHA canola, a genetically engineered Brassica napus (OECD Unique Identifier NS-B5ØØ27-4), has been developed as one of the first land-based production systems for omega-3 long-chain polyunsaturated fatty acids (LCPUFA), whose health benefits are well-established. Yet, the marine sources of these nutrients are under high pressures due to over-fishing and increasing demand. DHA canola is a plant-based source for these essential fatty acids that produces a high level of docosahexaenoic acid (DHA). This terrestrial system allows for sustainable, scalable and stable production of omega-3 LCPUFA that addresses not only the increasing market demand, but also the complex interplay of agriculture, aquaculture, and human nutrition. The vector used to produce the desired oil profile in DHA canola contains the expression cassettes of seven genes in the DHA biosynthesis pathway and was specifically designed to convert oleic acid to DHA in canola seed. The characterization and safety evaluation of food and feed produced from DHA canola are described and supported by a detailed nutritional analysis of the seed, meal, and oil. Aside from the intended changes of the fatty acid profile, none of the other compositional analytes showed biologically meaningful differences when compared to conventional canola varieties. In addition, the meal from DHA canola is compositionally equivalent to conventional canola meal. Further evidence of nutritional value and safety of DHA canola oil have been confirmed in fish feeding studies. Given that most human populations lack sufficient daily intakes of omega-3 LCPUFA, a dietary exposure assessment is also included. In conclusion, the results from these studies demonstrate it is safe to use products derived from DHA canola in human foods, nutraceuticals, or animal feeds.

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“…It, however, needs to be entered in the FASTA format [Pearson, 1988]. An additional database located on the FARRP website is a separate bioinformatic tool allowing to evaluate whether a protein belonging to the same subfamily as wheat gliadins, is similar enough to gluten to be able to induce symptoms typical of celiac disease Siruguri et al, 2015].…”

Section: Databases Of Allergensmentioning

confidence: 99%

Databases and Associated Bioinformatic Tools in Studies of Food Allergens, Epitopes and Haptens – a Review

Bucholska

Minkiewicz

Darewicz

et al. 2018

Pol. J. Food Nutr. Sci.

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Allergies and/or food intolerances are a growing problem of the modern world. Diffi culties associated with the correct diagnosis of food allergies result in the need to classify the factors causing allergies and allergens themselves. Therefore, internet databases and other bioinformatic tools play a special role in deepening knowledge of biologically-important compounds. Internet repositories, as a source of information on different chemical compounds, including those related to allergy and intolerance, are increasingly being used by scientists. Bioinformatic methods play a signifi cant role in biological and medical sciences, and their importance in food science is increasing. This study aimed at presenting selected databases and tools of bioinformatic analysis useful in research on food allergies, allergens (11 databases), epitopes (7 databases), and haptens (2 databases). It also presents examples of the application of computer methods in studies related to allergies.

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Evaluation of Bar, Barnase, and Barstar recombinant proteins expressed in genetically engineered Brassica juncea (Indian mustard) for potential risks of food allergy using bioinformatics and literature searches

Cited by 17 publications

References 35 publications

AllergenOnline: A peer‐reviewed, curated allergen database to assess novel food proteins for potential cross‐reactivity

AllergenOnline: A peer‐reviewed, curated allergen database to assess novel food proteins for potential cross‐reactivity

Food and Feed Safety of NS-B5ØØ27-4 Omega-3 Canola (Brassica napus): A New Source of Long-Chain Omega-3 Fatty Acids

Databases and Associated Bioinformatic Tools in Studies of Food Allergens, Epitopes and Haptens – a Review

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