Evaluation of baculovirus-derived recombinant 53-kDa protein of Trichinella spiralis for detection of Trichinella-specific antibodies in domestic pigs by ELISA

Jung, Doreen; Teifke, Jens Peter; Karger, Axel; Michael, Kathrin; Venz, Simone; Wittmann, Wolfgang; Kindermann, Katharina; Nöckler, Karsten; Mundt, Egbert

doi:10.1007/s00436-006-0296-7

Cited by 10 publications

(5 citation statements)

References 27 publications

(31 reference statements)

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“…is the smallest, but clinically important, nematode parasite that is widespread and a main causative agent of trichinellosis [ 1 , 2 ]. It is commonly observed in carnivorous mammals and omnivores, including pigs, rodents, and humans [ 3 – 5 ]. Trichinellosis has been reported in about 55 countries worldwide and is very common in developing countries [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

et al. 2018

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Trichinella spiralis mainly dwells in the muscle tissue of its host and is the main causative agent of trichinellosis in humans. Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, including resistance to T. spiralis. Herein, we applied label-free analysis to investigate the effect of sodium nitroprusside (SNP, a NO donor compound) on the proteome of T. spiralis muscle larvae (ML), followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analyses. Of the 1,476 proteins detected in the ML, 121 proteins showed differential expression, including 50 significantly upregulated and 71 downregulated proteins. The functions of the 108 annotated proteins were primarily related to signal transduction, transcription/translation, material metabolism, protein synthesis/assembly/degradation, and stress/defense/antioxidation. Quantitative real-time polymerase chain reaction (qRT-PCR) assay verified that FRMD5 and CUT-1 gene expression levels were significantly increased, while COX2 gene expression level was significantly decreased. GO annotation and KEGG pathway analyses showed that the majority of differentially expressed proteins were mainly involved in the molecular function of the catalytic activity, biological process of the immune system process, metabolic process, cellular component organization, biological adhesion, and cellular component of the macromolecular complex. Our results demonstrate the first comprehensive protein expression profile of the ML in response to NO stress and provide novel references for understanding the potential mechanism underlying the effects of NO on trichinellosis.

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

et al. 2018

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“…Gottstein et al (2009) revealed that in swine infected with 100 or 500 T. spiralis ML, the ML specific antibodies were detected from 5-7 weeks post infection (wpi) and 4-5 wpi, respectively. Moreover, several studies have emphasised that E-S ML proteins contain components that can cross-react with sera from persons affected by other diseases including echinococcosis or cysticercosis and thus may not give reliable results in diagnostics (Jung et al 2007, Gottstein et al 2009.…”

mentioning

confidence: 99%

Proteomic analysis of potential immunoreactive proteins from muscle larvae and adult worms of Trichinella spiralis in experimentally infected pigs

Bień¹,

Cabaj²,

Moskwa³

2015

Folia Parasitologica

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The present study was undertaken to identify potentially immunoreactive proteins of the muscle larvae (ML) and adult stage (Ad) of the nematode Trichinella spiralis Owen, 1835. To identify immunoreactive proteins that are specifically recognised by anti-Trichinella antibodies, ML and Ad crude extracts and their excretory-secretory (E-S) products were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot with serum samples from pigs experimentally infected with T. spiralis. A total of 18 bands were selected for final identification by liquid chromatography-tandem mass spectrometry. To further understand the functions of the proteins identified in this study, gene ontology terms were applied. Results showed that the specific antibodies against T. spiralis reacted with protein bands matching heat shock proteins, aminopeptidase, enolase, isocitrate dehydrogenase NADP-dependent, tropomyosin, P49 antigen, serine proteinase, secreted 5'-nucleotidase, antigen targeted by protective antibodies, 53 kDa E-S antigen, putative trypsin and paramyosin. Three proteins common for both adult stage and muscle larvae, including heat shock proteins, enolase and 5'-nucleotidase, might play important role during T. spiralis infection. These proteins are presumably presented to the host immune system and may induce humoral immune response. Thus, these proteins may be potential antigens for early diagnosis and the development of a vaccine against the parasite.

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“…Thus, ML ES antigens could be used to detect sera in late infection of Trichinella . More importantly, ML ES antigens cross-react with the sera of patients with other types of helminthiasis (10, 11). Therefore, it is necessary to screen sensitive and specific early diagnostic antigens of Trichinella .…”

Section: Introductionmentioning

confidence: 99%

Kinetics Evaluation of IgM and IgG Levels in the Mice Infect-ed with Trichinella spiralis Experimentally Using ES Antigens from Different Developmental Stages of the Parasite

Zhai¹,

Sun

Liu

et al. 2019

IJPA

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Background: To assay the Trichinella-specific IgM and IgG antibody responses during the early stage of infection, serum was collected from mice infected with the muscle larvae (ML) of T. spiralis (ISS534) at different dpi (days post infection) up to 60 days. Methods: The levels of IgM and IgG antibodies in serum were measured by ES antigens from different stage of T. spiralis using the ELISA method in Shanghai, China in 2017. Results: The anti-Trichinella IgM and IgG could be detected by ES antigens from the adult three days worm (Ad3) as early as 5 dpi and 8 dpi, respectively. ES antigens from the mixture of adult six days worm & new born larvae (Ad6+NBL) was similar to Ad3. When antibodies were detected by these two antigens, the levels of IgM peaked at 14 dpi and then declined from 15 dpi to 60 dpi; the IgG peaked at 20 dpi, and gradually declined, however, higher detection levels were maintained until 60 dpi. Conclusion: Ad3 ES antigens showed more antigenicity than Ad6+NBL ES on titer detection of IgM and IgG antibodies, and the production of Ad3 ES is easier. In terms of early diagnosis, these two antigens are better than the ML ES antigens of T. spiralis, which antibodies could not be detected before 20dpi. Ad3 ES antigens might be good candidate for the early diagnosis of trichinellosis or the mixture of Ad3 and Ad6+NBL ES might be used.

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Evaluation of baculovirus-derived recombinant 53-kDa protein of Trichinella spiralis for detection of Trichinella-specific antibodies in domestic pigs by ELISA

Cited by 10 publications

References 27 publications

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

Proteomic analysis of potential immunoreactive proteins from muscle larvae and adult worms of Trichinella spiralis in experimentally infected pigs

Kinetics Evaluation of IgM and IgG Levels in the Mice Infect-ed with Trichinella spiralis Experimentally Using ES Antigens from Different Developmental Stages of the Parasite

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