Evaluation of bacterial kill in human roots infected with <scp><i>Enterococcus faecalis</i></scp> biofilm following 980 nm laser activation of chlorhexidine

Jiang, Anna P.; Ipe, Deepak S.; Love, Robert M.; George, Roy

doi:10.1002/tbio.202000008

Cited by 2 publications

(2 citation statements)

References 32 publications

(41 reference statements)

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“…In 2020, Jiang et al . evaluated the bacterial eradication efficacy of CHX activated with a laser at the roots’ apical one-third and tested by using a 3D fluorescence microscope, they concluded that a greater number of bacteria were destroyed with laser-assisted irrigation immediately following treatment ( 13 ). This study aimed to investigate if agitating the root canals with an Erbium laser improved the efficiency of 2% CHX against mature E. faecalis biofilm in the lower human molar systems.…”

Section: Introductionmentioning

confidence: 99%

Eradication of mature biofilm from the isthmus region using Er, Cr: YSGG Laser as an activator of 2% chlorhexidine gluconate

Ibrahim,

Jawad

2024

J Clin Exp Dent

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Background Challenges in the root canal system, such as isthmus, may limit the action of endodontic equipment and irrigation solutions, so using laser agitation is recommended to upgrade the removal of microbial biofilm. The objective of this study is to assess the effectiveness of the laser in photon-induced photoacoustic streaming protocol agitation of 2% chlorohexidine gluconate in removing mature biofilm in complex root canal systems. Material and Methods Seventy-five mesial roots of the lower first and second molars were separated and cultivated with Enterococcus faecalis bacteria for 30 days (except for the negative control group samples). The samples were divided into four groups (n=15), one group acted as a positive control, other groups were irrigated with 2% chlorohexidine gluconate, some of them were agitated with a passive ultrasonic device, while the other samples were agitated by an Erbium, chromium-doped yttrium, scandium, gallium, and garnet laser in photon-induced photoacoustic streaming protocol. An atomic force microscope was used as a new method to get the results in the isthmus area; A scanning electron microscope was also used in the study to examine the samples before and after the treatment. Statistical Package for Social Sciences software was used to collect and analyze data, and two-way ANOVA was used to compare the means of the test groups. Results According to the atomic force microscope and scanning electron microscope analyses, Erbium laser and passive ultrasonic activation groups showed higher antimicrobial efficacy than the syringe irrigation group ( p <0.05). Conclusions According to the study’s results, the agitation of chlorohexidine gluconate fluid by Erbium laser in photon-induced photoacoustic streaming at 0.75 W offers better Enterococcus faecalis biofilm removal in the difficult-to-reach areas of lower first and second molars. Key words: Atomic force microscope, 2% chlorhexidine gluconate, Enterococcus faecalis, Erbium, chromium-doped yttrium, scandium, gallium, garnet laser, passive ultrasonic.

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Section: Introductionmentioning

confidence: 99%

Eradication of mature biofilm from the isthmus region using Er, Cr: YSGG Laser as an activator of 2% chlorhexidine gluconate

Ibrahim,

Jawad

2024

J Clin Exp Dent

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“…The most previous work on irrigation activation methods has been done about the improvement of biofilm removal by the laser agitation of chlorhexidine in an infected single root canal with Enterococcus faecalis biofilm obtained after a short incubation period [15,16]. The goal of this study was to examine whether mechanical agitation by Er,Cr:YSGG laser improves the effectiveness of 2% chlorhexidine against 30-day-old E. faecalis biofilm in human mandibular root canal systems, and use of AFM to investigate the impact of different irrigation protocols on the topography of the surface by analyzing isthmus surface properties.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of Er,Cr:YSGG and Ultrasonic on E. faecalisbiofilm in the mesial root canal systems of lower molars

IBRAHIM,

JAWAD

2024

Braz. Dent. Sci.

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The internal topography of the root canal is complex, especially for the permanent molar's mesial root. In response to such issues, improved irrigation techniques have been created, which use laser pulses to agitate fluids and improve microbial deposit removal. Objective: To assess the effectiveness of the Er,Cr:YSGG laser with a wavelength of 2,780 nm via photon-induced photoacoustic streaming (PIPS) protocol which agitated of 2% chlorohexidine (CHX) in removing mature Enterococcus faecalis (E. faecalis) biofilm in root canal systems of lower molars. Material and Methods:The mesial roots of lower first and second molars were separated and inoculated with E. faecalis bacterial suspension for 30 days. The roots were irrigated with CHX, some of them were agitated with a passive ultrasonic device (PUI), while the other roots were agitated by an Er,Cr:YSGG laser in PIPS at 60 µs/pulse, 5 Hz, (0.25, 0.5, 0.75, and 1) W. An atomic force microscope (AFM) was used as a new method to get the results in the isthmus area; the obtained results from each group were compared with each other. Results: Based on the AFM and SEM analyses, laser and ultrasonic activation groups showed higher antimicrobial efficacy than the conventional syringe irrigation group (P<0.05). Conclusion: Based on the investigation's findings, the activation of 2% CHX solution by Er,Cr:YSGG laser in PIPS and PUI offers better mature bacterial biofilm removal in the mesial root of lower human molars than the same irrigant with the SI technique.

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Evaluation of bacterial kill in human roots infected with Enterococcus faecalis biofilm following 980 nm laser activation of chlorhexidine

Cited by 2 publications

References 32 publications

Eradication of mature biofilm from the isthmus region using Er, Cr: YSGG Laser as an activator of 2% chlorhexidine gluconate

Eradication of mature biofilm from the isthmus region using Er, Cr: YSGG Laser as an activator of 2% chlorhexidine gluconate

Antimicrobial activity of Er,Cr:YSGG and Ultrasonic on E. faecalisbiofilm in the mesial root canal systems of lower molars

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