Evaluation of BACTEC MGIT 960 system for the second-line drugs susceptibility testing of Mycobacterium tuberculosis in China

Zhao, Lili; Xia, Qiang; Lin, Nan; Liu, Binbin; Zhao, Xiuqin; Liu, Zhiguang; Wan, Kanglin

doi:10.1016/j.mimet.2012.06.010

Cited by 8 publications

(6 citation statements)

References 7 publications

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“…The sensitivity, specificity, PPV, and NPV rates of the BACTEC MGIT 960 SL DST kit were all 100%. The results reported by Zhao et al [13] and Morcillo et al [11] were consistent with our results. On the other hand, Said et al [15] reported PPV of 18%, which is considerably lower than that in our study (100%).…”

Section: Discussionsupporting

confidence: 94%

“…The results reported by Morcillo et al [11], Van Ingen et al [10], Kim et al [14] and Zhao et al [13] were consistent with those of our study. (4 µg/mL) (5 µg/mL) (4 µg/mL) (10 µg/mL) (2 µg/mL) (0.5 µg/mL) (2 µg/mL) (1 µg/mL) (2.5 µg/mL) (2.5 µg/mL) (2 µg/mL) (0.5 µg/mL) (2 µg/mL) Q The sensitivity, specificity, PPV, and NPV rates of the BACTEC MGIT 960 SL DST kit compared with those of the agar proportion method employed using 10-µg/mL CAP were 100%, 95.3%, 60%, 100%, respectively; three strains were identified as resistant to CAP.…”

Section: Discussionsupporting

confidence: 93%

“…The sensitivity, specificity, PPV, and NPV rates of the BACTEC MGIT 960 SL DST kit were all 100%. Zhao et al [13] studied 321 isolates (sensitivity 100% and specificity 98.6%) and Kim et al [14] studied 193 clinical isolates (sensitivity 100%, specificity 95%) using the BACTEC MGIT 960 SL DST, and their results were consistent with our results. However, Said et al [15] evaluated 343 isolates using the BACTEC MGIT 960 method and reported sensitivity of 27%, which was considerably lower than that in our study.…”

Section: Discussionsupporting

confidence: 88%

“…This concentration caused a decrease in the number of resistant strains (from five resistant strains to three strains), resulting in a wrong assessment as the other two resistant strains detected using the BACTEC MGIT 960 SL DST kit were interpreted as "false resistant. " Thus, the PPV rate was considerably lower in our study than in many other studies [10,13]. Consequently, we believe that a CAP concentration of 4 µg/ mL WHO is more appropriate to use in the Middlebrook 7H10 agar proportion method [8].…”

Section: Discussionmentioning

confidence: 59%

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Evaluation of the BACTEC MGIT 960 SL DST Kit and the GenoType MTBDRsl Test for Detecting Extensively Drugresistant Tuberculosis Cases

Tekin¹,

Albay²,

Şimşek³

et al. 2017

Eurasian J Med

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Objective: The present study aimed to evaluate the performances of the BACTEC MGIT 960 SL DST kit and the GenoType MTBDRsl test for detecting second-line antituberculosis drug resistance in Multidrugresistant TB (MDR-TB) cases. Materials and Methods:Forty-six MDR-TB strains were studied. Second-line antituberculosis drug resistances were detected using the BACTEC MGIT 960 SL DST kit and the GenoType MTBDRsl test. The Middlebrook 7H10 agar proportion method was used as the reference test.Results: The sensitivity and specificity values for the BACTEC MGIT 960 SL DST kit were both 100% for amikacin, kanamycin, capreomycin (4 µg/mL), and ofloxacin; 100% and 95.3%, respectively, for capreomycin (10 µg/mL); and 85.7% and 100%, respectively, for moxifloxacin (0.5 µg/mL). The sensitivity and specificity values for the GenoType MTBDRsl test to detect fluoroquinolone and aminoglycoside/cyclic peptide resistance were 88.9% and 100%, respectively, for ofloxacin and 85.7% and 94.9%, respectively, for moxifloxacin (0.5 µg/ mL). The accuracy of the GenoType MTBDRsl assay for kanamycin, capreomycin, ofloxacin, and moxifloxacin was lower than that of the BACTEC MGIT 960 SL DST. Conclusion:The BACTEC MGIT 960 SL DST kit and the GenoType MTBDRsl were successful in detecting second-line antituberculosis drug resistance. Preliminary results of the GenoType MTBDRsl are very valuable for early treatment decisions, but we still recommend additional BACTEC MGIT 960 SL DST kit usage in the routine evaluation of drug-resistant tuberculosis.Keywords: Mycobacterium tuberculosis, agar proportion method, MDR-TB, second-line drug susceptibility ÖZ Amaç: Bu çalışmanın amacı, çok ilaca dirençli tüberküloz (ÇİD-TB) vakalarında, BACTEC MGIT 960 SL DST kitinin ve GenoType MTBDRsl testinin, ikincil antitüberküloz ilaçlara karşı duyarlılığı saptamasındaki performansının değerlendirilmesidir.Gereç ve Yöntem: 46 tane ÇİD-TB suşu çalışıldı. BACTEC MGIT 960 SL DST kiti ve GenoType MTBDRsl testi ile ikincil antitüberküloz ilaçlara karşı direnç durumu tespit edilmiştir. Middlebrook 7H10 agar proporsiyon metodu referans yöntem olarak kullanılmıştır.Bulgular: BACTEC MGIT 960 SL DST kitinin duyarlılık ve özgüllüğü; amikasin, kanamisin, kapreomisin (4 µg/ mL) ve ofloksasin için her ikisi de %100; kapreomisin (10 µg/mL) için sırasıyla %100 ve %95,3; moksifloksasin (0,5 µg/mL) için sırasıyla %85,7 ve 100% olarak bulunmuştur. GenoType MTBDRsl testinin florokinolon ve aminoglikozid/siklik peptit direnci tespitinde duyarlılık ve özgüllüğü; ofloksasin için sırasıyla %88,9 ve %100; moksifloksasin (0,5 µg/mL) için sırasıyla %85,7 ve %94,9 olarak bulunmuştur. GenoType MTBDRsl yönteminin kanamisin, kapreomisin, ofloksasin ve moksifloksasin için doğruluğu, BACTEC MGIT 960 SL DST kitine göre daha düşük saptanmıştır.Sonuç: BACTEC MGIT 960 SL DST kiti ve GenoType MTBDRsl testi ikincil antitüberküloz ilaçlara karşı direncin saptanmasında başarılı bulunmuştur. GenoType MTBDRsl testi ile elde edilen ön bilgiler, erken dönemde tedavi kararlarının alınmasında değerlidir,...

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 59%

See 2 more Smart Citations

Evaluation of the BACTEC MGIT 960 SL DST Kit and the GenoType MTBDRsl Test for Detecting Extensively Drugresistant Tuberculosis Cases

Tekin¹,

Albay²,

Şimşek³

et al. 2017

Eurasian J Med

View full text Add to dashboard Cite

show abstract

“…However, AP was likely to suffer from variations such as the method of manufacture and the critical concentrations of some drugs compared with L-J PM [23]. In China, the DST methods for MTB mainly used the absolute-concentration method and the PM on L-J medium [7] and this study was designed to compare these.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to First-Line Drugs in China

Zhao¹,

Fang

Yu³

et al. 2014

PLoS ONE

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BackgroundThe purpose of this study was to evaluate the performance of the BACTEC MGIT 960 (M960) system compared with the proportion method (PM) on Löwenstein-Jensen (L-J) medium in a peripheral laboratory in China for the testing of Mycobacterium tuberculosis (MTB) susceptibility to streptomycin (SM), isoniazid (INH) rifampicin (RIF) and ethambutol (EMB) a combination known as SIRE.MethodsThe susceptibility of 205 clinical isolates of MTB to SM, INH, RIF and EMB was performed with the M960 system. The drugs were tested at the following concentrations: 1.0 µg/ml for SM, 0.1 µg/ml for INH, 1.0 µg/ml for RIF, and 5.0 µg/ml for EMB. The results were compared with those obtained by the L-J PM. The L-J PM at an arbiter site was used to resolve any discordant results.ResultsThe overall consistency was 96.6% and concordance values were 95.6% for SM, 97.6% for INH, 98.0% for RIF and 95.1% for EMB. The overall sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the M960 system for PM (the standard method) was 95.6%, 97.3%, 96.2% and 96.9% respectively, and the sensitivity were 93.3% for SM, 96.9% for INH, 97.4% for RIF and 94.6% for EMB, the specificity were 96.9% for SM, 98.2% for INH, 98.4% for RIF and 95.5% for EMB, the PPV were 94.6% for SM, 97.9% for INH, 97.4% for RIF and 94.6% for EMB, the NPV were 96.2% for SM, 97.3% for INH, 98.4% for RIF and 95.5% for EMB. The turnaround time with the M960 system (median 8.0 days, ranged from 5 to 14 days) was significantly shorter than that with the PM (28 days or 42 days).ConclusionThere was a substantial degree of agreement between the two methods. The M960 system was a reliable and rapid method for SIRE susceptibility testing of tuberculosis in China.

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Multicenter Research on the BACTEC MGIT 960 system for the second-line drugs susceptibility testing of Mycobacterium tuberculosis in China

Zhao

Liang²,

Huang

et al. 2013

Diagnostic Microbiology and Infectious Disease

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Evaluation of BACTEC MGIT 960 system for the second-line drugs susceptibility testing of Mycobacterium tuberculosis in China

Cited by 8 publications

References 7 publications

Evaluation of the BACTEC MGIT 960 SL DST Kit and the GenoType MTBDRsl Test for Detecting Extensively Drugresistant Tuberculosis Cases

Evaluation of the BACTEC MGIT 960 SL DST Kit and the GenoType MTBDRsl Test for Detecting Extensively Drugresistant Tuberculosis Cases

Evaluation of BACTEC MGIT 960 System for Testing Susceptibility of Mycobacterium tuberculosis to First-Line Drugs in China

Multicenter Research on the BACTEC MGIT 960 system for the second-line drugs susceptibility testing of Mycobacterium tuberculosis in China

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