Evaluation of Bact/Alert Virtuo for Detection of Bacteria in Platelet Concentrate

In Japan, transfusion-transmitted bacterial infection remains an ongoing problem, and further preventive measures are necessary to ensure blood safety. Here, we developed a bacterial 16S rDNA real-time PCR assay (PCR assay) and compared its detectability with that of a fully automated blood culture system, BacT/ALERT VIRTUO (VIR-TUO) by testing bacteria-spiked platelet concentrate (PC). All PC samples stored for 40 hours after inoculation with 4 bacterial species with a total of 8 strains tested positive by both methods, except for three samples with low bacterial concentrations under the detection limit (10 CFU/ml ) by the agar plate culture method. However, PCR assay may have lower detectability than VIRTUO when testing slow-growing bacteria in PC. This PCR assay has a shorter running time than VIRTUO of about 3 hours, and incorporation of internal control allows the monitoring of false-negative results by PCR inhibitors.Further development of bacterial DNA-free PCR reagents together with an automatic assay system will likely make this PCR assay a practical bacterial screening method.

show abstract

血小板製剤の細菌検査としての細菌16S rDNAリアルタイムPCR法の評価

Yoshimasa

Matsumoto

Ikeda

et al. 2023

JJTC

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Evaluation of Bact/Alert Virtuo for Detection of Bacteria in Platelet Concentrate

Cited by 1 publication

References 17 publications

血小板製剤の細菌検査としての細菌16S rDNAリアルタイムPCR法の評価

血小板製剤の細菌検査としての細菌16S rDNAリアルタイムPCR法の評価

Contact Info

Product

Resources

About