Evaluation of background DNA damage in a Turkish population measured by means of the cytokinesis-block micronucleus cytome assay

Çoşkun, Mahmut; Çayır, Akın; Tok, Hayal

doi:10.1016/j.mrgentox.2013.03.010

Cited by 26 publications

(17 citation statements)

References 24 publications

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“…The result of this study is consistent with previous studies reporting a higher MN frequency in females relative to males [Fenech and Bonassi, 2011]. The higher NPB frequency in males reported in our investigation is in agreement with results from a study on exposure to polycyclic aromatic hydrocarbons (PAH) which also showed that male NPB frequencies were significantly increased relative to females [Duan et al, 2009], however, another study evaluating the background incidence of DNA damage in Turkey has reported that the frequency of occurrence of NPB was significantly higher in females [Coşkun et al, 2013]. These inconsistencies with regard to the gender effect on NPB frequency require further investigation.…”

Section: Discussionsupporting

confidence: 93%

Chromosomal DNA damage measured using the cytokinesis‐block micronucleus cytome assay is significantly associated with cognitive impairment in South Australians

Lee

Thomas

Hecker

et al. 2014

Environ and Mol Mutagen

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Loss of genome integrity may be associated with increased risk for neurodegenerative disease. The aim of this study was to investigate whether mild cognitive impairment (MCI) or Alzheimer's disease (AD) individuals have increased DNA damage relative to age- and gender- matched controls using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. DNA damage was measured as micronuclei (MN), nucleoplasmic bridges (NPB), and nuclear buds (NBUD) in binucleated cells. The assay was performed on blood samples from 80 participants consisting of (i) MCI cases (N = 20) and age- and gender- matched controls (N = 20), and (ii) AD cases (N = 20) and age- and gender- matched controls (N = 20). There was a significant increase in MCI NBUD frequency (P = 0.006) relative to controls, which was also observed in male (P = 0.03) and female (P = 0.04) subgroups. For AD cases, there were no significant differences in assay biomarkers relative to controls. There was a significant negative correlation between Mini Mental State Examination (MMSE) and (i) MN in all controls, (R = -0.3, P = 0.04), and AD cases (R = -0.4, P = 0.03), (ii) NPB in all controls, (R = -0.4, P = 0.006) and AD cases (R = -0.5, P = 0.01), and (iii) NBUD in MCI cases (R = -0.5, P = 0.007) and AD cases (R = -0.7, P = 0.0002). The results suggest that an increase in lymphocyte CBMN-Cyt DNA damage biomarkers may be associated with cognitive decline.

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Section: Discussionsupporting

confidence: 93%

Chromosomal DNA damage measured using the cytokinesis‐block micronucleus cytome assay is significantly associated with cognitive impairment in South Australians

Lee

Thomas

Hecker

et al. 2014

Environ and Mol Mutagen

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“…In general, the higher MN frequencies of females have been attributed to micronucleation of the X chromosome [Fenech et al, ; Mateuca et al, ]. This study indicated similar results to previous studies [Coskun et al, ]. The NPB frequencies of females were higher than males but did not reach a statistically significant level.…”

Section: Discussionsupporting

confidence: 89%

“…With aging, the efficiency of the DNA repair process declines and the accumulation of mutations increases, which is observed in chromosomal damage [Wojda et al, ]. Aging has been shown to be a major confounding factor that affects MN formation in a healthy population [Mateuca et al, ; Coskun et al, ]. Similarly, this association was found to be true in a population exposed to chemicals occupationally and environmentally.…”

Section: Discussionmentioning

confidence: 99%

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

Çayır

Çoşkun

Çobanoğlu

2017

Environ and Mol Mutagen

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Pesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis-block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf-DNA) in the blood of pesticide-exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf-DNA levels using a Qubit fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide-exposed individuals were significantly higher than non-exposed individuals. Apart from MNL, BNMN and CBPI, a season-related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf-DNA level in the pesticide-exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf-DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf-DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf-DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161-169, 2018. © 2017 Wiley Periodicals, Inc.

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“…Results on the effects of smoking are also conflicting with some studies reporting no effect of smoking on MN frequency [10,13]. Kažimirova et al (2006) did not find significant differences in micronucleus frequency between vegetarians and non-vegetarians across different age groups [17].…”

Section: Introduction mentioning

confidence: 91%

“…According to [15] factors that affect MN frequency in healthy subjects are: type of cell line, gender, age, genotype, smoking, species and diet. For gender, results are conflicting: some studies report higher MN in women than in men [8,10,12,1619] but some studies report no gender effect on MN frequency [13]. MN frequency increases with age.…”

Section: Introduction mentioning

confidence: 99%

Structure of Relations Between the Frequency of Micronuclei in Peripheral Blood Lymphocytes and Age, Gender, Smoking Habits and Socio-Demographic Factors in South-East Region of Serbia

et al. 2019

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Frequency of micronuclei (MN) in peripheral blood lymphocytes is a measure of genotoxicity and spontaneous chromosomal instability with many modifying factors. The aim of this work was to examine the relation between results of micronucleus test and set of socio-demographic parameters in healthy population in south-east Serbia using structure equation approach. Cytokinesis–block (CB) technique was used for analysis of MN frequency in peripheral blood lymphocytes on 133 healthy volunteers of various ages. Socio-demographic data was collected through a questionnaire. The mean frequency of MN gradually rose with age from 0.56±0.71 in new-borns to 5.48 ±3.65 in the 61-80 years age group (AG), with a decrease in the 81-92 years AG. MN frequency was positively correlated with age, altitude of birth place, altitude of place of residence, nuclear buds and nucleoplasmatic bridges, and negatively with education level and smoking habits. Linear structural model revealed age to be related to all of the examined variables, and indicates probable existence of another factor, independent of age, influencing all of these except nuclear buds. It can be concluded that the frequency of micronuclei is influenced by age and factor/s resembling socioeconomic status or lifestyle and this influence is independent of age.

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Evaluation of background DNA damage in a Turkish population measured by means of the cytokinesis-block micronucleus cytome assay

Cited by 26 publications

References 24 publications

Chromosomal DNA damage measured using the cytokinesis‐block micronucleus cytome assay is significantly associated with cognitive impairment in South Australians

Chromosomal DNA damage measured using the cytokinesis‐block micronucleus cytome assay is significantly associated with cognitive impairment in South Australians

DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides

Structure of Relations Between the Frequency of Micronuclei in Peripheral Blood Lymphocytes and Age, Gender, Smoking Habits and Socio-Demographic Factors in South-East Region of Serbia

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