Evaluation of Bacillus velezensis NKG-2 for bio-control activities against fungal diseases and potential plant growth promotion

Myo, Ei Mon; Liu, Binghua; Ma, Jinjin; Shi, Liming; Jiang, Mingguo; Zhang, Kecheng; Ge, Beibei

doi:10.1016/j.biocontrol.2019.03.017

Cited by 99 publications

(50 citation statements)

References 41 publications

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“…In soil drench method, the LWI was significantly reduced to 13% and the biocontrol efficacy was 79.197%, indicating that strain SZAD2 was more effective in the soil drench method than seed treatment. These results are in accordance with previous studies reporting some B. velezensis strains as potential candidates for biocontrol under greenhouse conditions, such as B. velezensis strain NKG-2 had shown biocontrol potential against Botrytis cinerea in tomatoes (Myo et al 2019), and our findings had indicated that the correlation between in vitro and in planta was correspondent as documented by Gupta et al (2010).…”

Section: Bio-efficacy Under Greenhouse Conditionssupporting

confidence: 93%

A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification

Sherzad

Tang

2020

Egypt J Biol Pest Control

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Background Verticillium dahliae is one of the most destructive pathogens in many cotton-growing countries around the globe. There is still the lack of high-resistant upland cotton cultivars against this pathogen, and the usage of fungicides is threatening the environment. Biocontrol is an environment-friendly and a green technique in plant protection. In this regard, the present work was aimed to investigate antagonistic bacterial strains and to introduce them as new bioinoculants against V. dahliae in cotton. Main body of the abstract Numerous bacteria were isolated from cotton roots, and their antagonism was examined against V. dahliae. Strain SZAD2 which was identified as Bacillus velezensis on the bases of 16S rRNA gene analysis had significantly high biocontrol potential both in plate assay and under greenhouse conditions. The biocontrol efficacy in seed treatment was 60.31%, while in the soil drench method, it was 79.19%. Transmission electron microscopy (TEM) analysis revealed that the strain systemically colonized the roots and lived within the intercellular space. Hydrogen peroxide staining exposed that the strain increased plant basal defense response by the accumulation of hydrogen peroxide (H2O2) in both roots and leaves. Furthermore, the bacterization of cotton roots triggered induced systemic resistance (ISR) in the roots and caused the activation of the antioxidant enzymes such as phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), and phenol contents. Moreover, the antifungal extract produced by the strain contained the activity of both cellulase and chitinase, which were able to suppress fungal mycelia both in the presence and absence of cotton. Short conclusions Strain SZAD2 systemically colonized the plant, showed multiple modes of action against the pathogen which played a vital role in V. dahliae control, and is recommended for commercial exploration as a safe alternative bioinoculant instead of hazardous chemical fungicides.

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Section: Bio-efficacy Under Greenhouse Conditionssupporting

confidence: 93%

A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification

Sherzad

Tang

2020

Egypt J Biol Pest Control

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“…Very similar results were obtained by Nie et al (2017) [ 90 ] and Ahn et al (2007) [ 83 ], who highlighted the fundamental role of the JA/ET-mediated signaling pathway in combating B. cinerea and P. syringae pv tomato. Elicitors synthesized by PGPR microorganisms capable of activating ISR include the lipopeptides surfactin, bacillomycin and phengicine, as well as the volatile organic compound 2,3-butanediol [ 21 , 28 , 35 , 79 ]. Foliar and radicular applications of strain XT1 on infected strawberry and tomato plants (XT1+Bc) modified phytohormone content considerably, with a high increase in JA levels.…”

Section: Discussionmentioning

confidence: 99%

“…In recent decades, the species B. velezensis [ 19 ] has become one of the most, if not the most, commonly used PGPR [ 20 ]. Some of the strains belonging to this species are capable of producing a wide variety of metabolites, such as siderophores [ 21 ], enzymes [ 18 ], volatile organic compounds (VOC) and lipopeptides [ 22 , 23 , 24 , 25 , 26 , 27 , 28 ], which contribute to both their biofertilizing activity and biocontrol capacity through direct competition, by activating induced systemic resistance (ISR) and by modifying the host–plant microbiome [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Crop Protection against Botrytis cinerea by Rhizhosphere Biological Control Agent Bacillus velezensis XT1

Toral¹,

Rodríguez

Béjar

et al. 2020

Microorganisms

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This study aims to evaluate the use of Bacillus velezensis strain XT1 as a plant growth-promoting rhizobacterium (PGPR) and biocontrol agent against B. cinerea in tomato and strawberry plants. Foliar and radicular applications of strain XT1 increased plant total biomass as compared to the control and B. cinerea-infected plants, with root applications being, on the whole, the most effective mode of treatment. Applications of the bacterium were found to reduce infection parameters such as disease incidence and severity by 50% and 60%, respectively. We analyzed stress parameters and phytohormone content in order to evaluate the capacity of XT1 to activate the defense system through phytohormonal regulation. Overall, the application of XT1 reduced oxidative damage, while the H2O2 and malondialdehyde (MDA) content was lower in XT1-treated and B. cinerea-infected plants as compared to non-XT1-treated plants. Moreover, treatment with XT1 induced callose deposition, thus boosting the response to pathogenic infection. The results of this study suggest that the signaling and activation pathways involved in defense mechanisms are mediated by jasmonic acid (JA) and ethylene hormones, which are induced by preventive treatment with XT1. The study also highlights the potential of preventive applications of strain XT1 to activate defense mechanisms in strawberry and tomato plants through hormone regulation.

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“…Plant-growth-promoting PSMs control plant diseases caused by pathogens through various mechanisms like competitive root colonization, synthesis of lytic enzymes and allelochemicals, and detoxification of virulence factors and induce systemic resistance against plant pathogens (Compant et al 2005;Paul and Sinha 2017). Many phosphate-solubilizing genera like Pseudomonas, Serratia, Bacillus, and Streptomyces secrete antifungal metabolites like viscosinamide, peptaibols, daucans, gliovirin, terpenoids, polyketides, pyrrolnitrin, and phenazines that downregulate pathogenesis in the host plant (Myo et al 2019). Another study unveiled that Pseudomonas putida PSDM3, Enterobacter hormaechei PSDM10, and Advenella sp.…”

Section: Phosphate-solubilizing Microorganisms (Psms)mentioning

confidence: 99%

Phosphate-Solubilizing Microorganisms: Mechanism and Their Role in Phosphate Solubilization and Uptake

Rawat

Das

Shankhdhar

et al. 2020

J Soil Sci Plant Nutr

280

130

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Phosphorus is the second most critical macronutrient after nitrogen required for metabolism, growth, and development of plants. Despite the abundance of phosphorus in both organic and inorganic forms in the soil, it is mostly unavailable for plant uptake due to its complexation with metal ions in the soil. The use of agrochemicals to satisfy the demand for phosphorus to improve crop yield has led to the deterioration of the ecosystem and soil health, as well as an imbalance in the soil microbiota. Consequently, there is a demand for an alternate cost-effective and eco-friendly strategy for the biofortification of phosphorus. One such strategy is the application of phosphate-solubilizing microorganisms which can solubilize insoluble phosphates in soil by different mechanisms like secretion of organic acids, enzyme production, and excretion of siderophores that can chelate the metal ions and form complexes, making phosphates available for plant uptake. These microbes not only solubilize phosphates but also promote plant growth and crop yield by producing plant-growth-promoting hormones like auxins, gibberellins, and cytokinins, antibiosis against pathogens, 1-aminocyclopropane-1-carboxylic acid deaminase which enhances plant growth under stress conditions, improving plant resistance to heavy metal toxicity, and so on. Pyrroloquinoline quinine (pqq) and glucose dehydrogenase (gcd) are the representative genes for phosphorus solubilization in microorganisms. The content presented in this review paper focuses on different mechanisms and modes of action of phosphate-solubilizing microorganisms, their contribution to phosphorus solubilization, growth-promoting attributes in plants, and the molecular aspects of phosphorus solubilization.

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Evaluation of Bacillus velezensis NKG-2 for bio-control activities against fungal diseases and potential plant growth promotion

Cited by 99 publications

References 41 publications

A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification

A new strain of Bacillus velezensis as a bioagent against Verticillium dahliae in cotton: isolation and molecular identification

Crop Protection against Botrytis cinerea by Rhizhosphere Biological Control Agent Bacillus velezensis XT1

Phosphate-Solubilizing Microorganisms: Mechanism and Their Role in Phosphate Solubilization and Uptake

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