Evaluation of B. subtilis SPB1 biosurfactants' potency for diesel-contaminated soil washing: optimization of oil desorption using Taguchi design

Mnif, Inès; Sahnoun, Rihab; Ellouze-Chaâbouni, Semia; Ghribi, Dhouha

doi:10.1007/s11356-013-1894-4

Cited by 60 publications

(44 citation statements)

References 44 publications

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“…Culture conditions were carried out as described by Mnif et al (2013). At the end of the cultivation, the culture was centrifuged at 10,000×g and 4°C for 20 min to remove bacterial cells.…”

Section: Preparation Of the Lipopeptide Biosurfactantmentioning

confidence: 99%

“…At the end of the cultivation, the culture was centrifuged at 10,000×g and 4°C for 20 min to remove bacterial cells. The supernatant-free cells served for biosurfactant extraction during three consecutive cycles of acid precipitationdissolution as described by Mnif et al (2013). First, the cellfree supernatant was subjected to acid precipitation using concentrated HCl until pH=2, and left overnight at 4°C.…”

Section: Preparation Of the Lipopeptide Biosurfactantmentioning

confidence: 99%

“…The biodecolorization parameters including the concentration of the sucrose, the concentration of yeast extract, the concentration of SPB1 biosurfactant, inoculum density, and agitation time were studied, and their levels are presented in Table 1. The orthogonal array was chosen to determine the experimental plan, L 16 , as shown in Table 2 (Mnif et al 2013;Phadke 1989;Peace Stuart 1995). It was generated using Nemrod-W Version 2007 software (LPRAI, Marseille, France).…”

Section: Decolorization Optimizationmentioning

confidence: 99%

“…Experimental planification methodology was previously adopted as an efficient tool in improving biosurfactant production by our selected strain, B. subtilis SPB1 (Ghribi et al 2012;Mnif et al 2012a, b). The Taguchi method is a fractional factorial design that can show process variability and stability (Mnif et al 2013). It provides an economical way of simultaneously studying the effects of many parameters on process mean and variance (Mnif et al 2013).…”

Section: Decolorization Optimizationmentioning

confidence: 99%

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Improvement of methyl orange dye biotreatment by a novel isolated strain, Aeromonas veronii GRI, by SPB1 biosurfactant addition

Mnif

Maktouf

Fendri

et al. 2015

Environ Sci Pollut Res

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Aeromonas veronii GRI (KF964486), isolated from acclimated textile effluent after selective enrichment on azo dye, was assessed for methyl orange biodegradation potency. Results suggested the potential of this bacterium for use in effective treatment of azo-dye-contaminated wastewaters under static conditions at neutral and alkaline pH value, characteristic of typical textile effluents. The strain could tolerate higher doses of dyes as it was able to decolorize up to 1000 mg/l. When used as microbial surfactant to enhance methyl orange biodecolorization, Bacillus subtilis SPB1-derived lipopeptide accelerated the decolorization rate and maximized slightly the decolorization efficiency at an optimal concentration of about 0.025%. In order to enhance the process efficiency, a Taguchi design was conducted. Phytotoxicity bioassay using sesame and radish seeds were carried out to assess the biotreatment effectiveness. The bacterium was able to effectively decolorize the azo dye when inoculated with an initial optical density of about 0.5 with 0.25% sucrose, 0.125% yeast extract, 0.01% SPB1 biosurfactant, and when conducting an agitation phase of about 24 h after static incubation. Germination potency showed an increase toward the nonoptimized conditions indicating an improvement of the biotreatment. When comparing with synthetic surfactants, a drastic decrease and an inhibition of orange methyl decolorization were observed in the presence of CTAB and SDS. The nonionic surfactant Tween 80 had a positive effect on methyl orange biodecolorization. Also, studies ensured that methyl orange removal by this strain could be due to endocellular enzymatic activities. To conclude, the addition of SPB1 bioemulsifier reduced energy costs by reducing effective decolorization period, biosurfactant stimulated bacterial decolorization method may provide highly efficient, inexpensive, and time-saving procedure in treatment of textile effluents.

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Section: Preparation Of the Lipopeptide Biosurfactantmentioning

confidence: 99%

Section: Preparation Of the Lipopeptide Biosurfactantmentioning

confidence: 99%

Section: Decolorization Optimizationmentioning

confidence: 99%

Section: Decolorization Optimizationmentioning

confidence: 99%

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Improvement of methyl orange dye biotreatment by a novel isolated strain, Aeromonas veronii GRI, by SPB1 biosurfactant addition

Mnif

Maktouf

Fendri

et al. 2015

Environ Sci Pollut Res

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“…subtilis SPB1. The used SPB1 biosurfactant was produced and prepared according to the protocol described in our previous work (Mnif et al 2013a).…”

Section: Growth Kinetics Of the Selected Consortium Under Different Cmentioning

confidence: 99%

Biodegradation of diesel oil by a novel microbial consortium: comparison between co-inoculation with biosurfactant-producing strain and exogenously added biosurfactants

Mnif

Sahnoun

et al. 2015

Environ Sci Pollut Res

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103

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Bioremediation, involving the use of microorganisms to detoxify or remove pollutants, is the most interesting strategy for hydrocarbon remediation. In this aim, four hydrocarbon-degrading bacteria were isolated from oil-contaminated soil in Tunisia. They were identified by the 16S rDNA sequence analysis, as Lysinibacillus bronitolerans RI18 (KF964487), Bacillus thuringiensis RI16 (KM111604), Bacillus weihenstephanensis RI12 (KM094930), and Acinetobacter radioresistens RI7 (KJ829530). Moreover, a lipopeptide biosurfactant produced by Bacillus subtilis SPB1, confirmed to increase diesel solubility, was tested to increase diesel biodegradation along with co-inoculation with two biosurfactant-producing strains. Culture studies revealed the enhancement of diesel biodegradation by the selected consortium with the addition of SPB1 lipopeptide and in the cases of co-inoculation by biosurfactant-producing strain. In fact, an improvement of about 38.42 and 49.65 % of diesel degradation was registered in the presence of 0.1 % lipopeptide biosurfactant and when culturing B. subtilis SPB1 strain with the isolated consortium, respectively. Furthermore, the best improvement, evaluated to about 55.4 %, was recorded when using the consortium cultured with B. subtilis SPB1 and A. radioresistens RI7 strains. Gas chromatography analyses were correlated with the gravimetric evaluation of the residual hydrocarbons. Results suggested the potential applicability of the selected consortium along with the ex situ- and in situ-added biosurfactant for the effective bioremediation of diesel-contaminated water and soil.

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Potential application of Bacillus subtilis SPB1 biosurfactants in laundry detergent formulations: Compatibility study with detergent ingredients and washing performance

Bouassida

Fourati

Ghazala

et al. 2017

Engineering in Life Sciences

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Surfactants play a very important role in laundry and household cleaning products ingredients. In this research, the application of lipopeptide biosurfactants, produced by Bacillus subtilis SPB1, in the formulation of a washing powder was investigated. The SPB1 biosurfactant was mixed with sodium tripolyphosphate as a builder and sodium sulfate as filler. The efficiency of the formulated detergent composition with different washing conditions to remove a stain from cotton fabric was examined. The results showed that the formulated detergent was effective in oil removal, with optimal washing conditions of pH, temperature, striate and time of washing system of 7, 65°C, 1000 RPM and 60 min, respectively. A comparative study of different detergent compositions (biosurfactant-based detergent, combined biosurfactantcommercial detergent, and a commercial detergent) for the removal of oil and tea stains, proved that the bio-scouring was more effective (>75%) in terms of the stain removal than the commercial powders (<60%). Moreover, the results demonstrated that the biosurfactant acts additively with a commercial detergent and enhances their performance from 33 to 45% in removing oil stain and from 57 to 64% in removing tea stain. As a conclusion, in addition to the low toxicity and the high biodegradability of the microbial biosurfactants, the results of this study have shown that the future use of this lipopeptide biosurfactant as laundry detergent additive is highly promising.

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Evaluation of B. subtilis SPB1 biosurfactants' potency for diesel-contaminated soil washing: optimization of oil desorption using Taguchi design

Cited by 60 publications

References 44 publications

Improvement of methyl orange dye biotreatment by a novel isolated strain, Aeromonas veronii GRI, by SPB1 biosurfactant addition

Improvement of methyl orange dye biotreatment by a novel isolated strain, Aeromonas veronii GRI, by SPB1 biosurfactant addition

Biodegradation of diesel oil by a novel microbial consortium: comparison between co-inoculation with biosurfactant-producing strain and exogenously added biosurfactants

Potential application of Bacillus subtilis SPB1 biosurfactants in laundry detergent formulations: Compatibility study with detergent ingredients and washing performance

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