Evaluation of B and T lymphocytes and plasma cells in colonic mucosa from healthy dogs and from dogs with inflammatory bowel disease

Stonehewer, Jo; Simpson, James; Else, R. W.; MacIntyre, Neil R.

doi:10.1016/s0034-5288(98)90028-5

Cited by 48 publications

(38 citation statements)

References 39 publications

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“…Afterwards, the same researcher studied the large intestine of canines with IBD and noted an increase of IgA+ and IgG+ plasma cells and T cells (Jergens et al 1999b). Similar results were obtained by Stonehewer et al (1998) who described the percentage of plasma cells and T cells in the lamina propria. Healthy dogs had significantly lower numbers of T cells and B cells in the lamina propria and also also lower numbers of T cells in the grandular epithelium.…”

Section: Immunohistochemical Markerssupporting

confidence: 88%

Biomarkers in canine inflammatory bowel disease diagnostics

Wdowiak¹,

Rychlik²,

Kołodziejska-Sawerska³

2013

Polish Journal of Veterinary Sciences

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Canine inflammatory bowel disease (IBD) is a heterogeneous group of chronic gastrointestinal disorders. The etiology, similar to human IBD, remains unknown. Canine IBD is diagnosed by exclusion, which is a long, time and money-consuming process due to the need of elimination of other diseases presenting with similar symptoms. Therefore, a search for a specific and sensitive marker is needed to overcome these difficulties.The article is divided into 3 sections presenting up-to-date information about laboratory markers, immunohistochemical markers and changes in the neurochemical coding of the enteric nervous system, concentrating on their usefulness and future applications. Data concerning laboratory and immunohistochemical markers is based mainly on canine IBD, while the neuroimmunohistochemistry section presents knowledge from human IBD due to the lack of such studies in veterinary medicine.

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Section: Immunohistochemical Markerssupporting

confidence: 88%

Biomarkers in canine inflammatory bowel disease diagnostics

Wdowiak¹,

Rychlik²,

Kołodziejska-Sawerska³

2013

Polish Journal of Veterinary Sciences

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“…The aetiopathogenesis of this chronic canine enteropathy, which can occur as an isolated clinical entity or concurrently with lymphocytic-plasmacytic enteritis (Jergens 1999), is poorly defined, although it is likely that immunologic, environmental, and genetic factors contribute (German et al 2003). Recent investigations into canine IBD have revealed changes in immune cell populations and inflammatory cytokine mRNA expression in both the small-and large-intestinal mucosa (Stonehewer et al 1998;German et al 2000German et al ,2001Ridyard et al 2002;Peters et al 2004), but the events leading up to these alterations remain poorly understood.…”

mentioning

confidence: 99%

Apical Junction Complex Protein Expression in the Canine Colon

Ridyard

Brown

Rhind

et al. 2007

J Histochem Cytochem.

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S U M M A R Y Canine idiopathic lymphocytic-plasmacytic colitis (LPC) is a well-recognized clinical and pathological entity in the dog, associated with altered immune cell populations and cytokine expression profiles. Clinical and experimental data indicate that alterations in the permeability of the intestinal epithelium contribute to the pathogenesis of a range of related conditions. The apical junction complex plays a significant role in regulating epithelial paracellular permeability, and we have characterized the distribution of a number of its component tight junction (ZO-1, occludin, claudin-2) and adherens junction (E-cadherin and b-catenin) proteins in normal colon and colon from dogs with idiopathic LPC. ZO-1, occludin, E-cadherin, and b-catenin exhibited a distribution in normal canine colon similar to that described previously in humans and rodents. In contrast to the situation in humans, claudin-2-specific labeling was observed in the normal canine colonic crypt epithelium, decreasing in intensity from the distal to the proximal crypt and becoming barely detectable at the luminal surface of the colon. There was little evidence for significant changes in ZO-1, occludin, E-cadherin, or b-catenin expression in dogs affected by idiopathic LPC. However, claudin-2 expression markedly increased in the proximal crypt and luminal colonic epithelium in affected dogs, suggesting a role in the pathogenesis of canine LPC.

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“…In previous studies, CD3 + lymphocytes in the LE were specified by numbers of IEL per 100 enterocytes [7,14,16,17]. In contrast, counting the CD3 + cells per area using an image analyzing software program or a grid may prove to be easier to use in diagnostical analysis.…”

Section: Discussionmentioning

confidence: 99%

“…This inflammation is further classified by the type of cellular infiltrate (neutrophilic, eosinophilic, lymphocytic, plasmacytic and granulomatous), where lymphocytic-plasmacytic infiltration is the most common form in dogs [5]. Increased numbers of CD3 + lymphocytes in lamina propria (LP) and lamina epithelialis (LE) have been documented in lymphocytic colitis [6][7][8][9] in the duodenum of dogs with IBD [9,10], and in histiocytic ulcerative colitis [11]. In contrast to these studies, a reduced number of CD3 + cells were found in the small intestine of dogs with IBD [12].…”

Section: Introductionmentioning

confidence: 99%

Horizontal and Vertical Distribution of CD3+ Lymphocytes in the Intestine of Healthy Adult and Neonatal Dogs

Pfammatter¹,

Luckschander²,

Jakob³

et al. 2017

J Clin Gastroenterol Hepatol

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T-lymphocytes are considered to play an essential role in the pathogenesis of inflammatory bowel disease. The goal of this study was an objective determination of the distribution of gastrointestinal CD3 + lymphocytes in a standardized dog population as a gold standard for further investigations. Full thickness biopsies were obtained from seven different localizations from stomach to colon from six adults and four neonatal healthy Beagle dogs. The tissue was stained with an anti-CD3 antibody. The positive cells were counted in an area of 200,000 µm 2 at four different sites per localization. In adult dogs, the horizontal distribution showed a maximum of CD3 + lymphocytes in the duodenum and jejunum. In the stomach, almost no positive cells were observed. The vertical distribution revealed an accumulation in the villi. The neonatal dogs showed a similar distribution pattern, but on average ten times less CD3 + lymphocytes in the analogous localizations and smaller differences between localizations.

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Evaluation of B and T lymphocytes and plasma cells in colonic mucosa from healthy dogs and from dogs with inflammatory bowel disease

Cited by 48 publications

References 39 publications

Biomarkers in canine inflammatory bowel disease diagnostics

Biomarkers in canine inflammatory bowel disease diagnostics

Apical Junction Complex Protein Expression in the Canine Colon

Horizontal and Vertical Distribution of CD3+ Lymphocytes in the Intestine of Healthy Adult and Neonatal Dogs

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