Evaluation of autotrophic and mixotrophic regimen Chlorella pyrenoidosa cells in various wastes water for its biochemical composition and biomass production

Abstract

The present study investigates the possibility of integrating an existing industrial large scale biomass production with the treatment of waste water in which a mixture of organic and inorganic rich pollutants was used as a medium. This study suggests that the replacement of a defined medium with a complete mixotrophic medium gives a significant statistical difference in terms of growth parameters i.e. biomass production and specific growth rate.The green microalga C. pyrenoidosa was cultivated under different mixotrophic conditions for evaluation of biomass production. Inorganic defined fog's medium supplemented, with raw dairy wastewater led to 1.37g/L biomass production in comparison to 1.2g/L obtained with pure glucose revealing 14.16% increase. The study also involves the supplementation of raw dairy wastewater as an organic carbon source in an inorganic medium comprising municipal treated water and reverse osmosis (RO) treated wastewater and attained 2.4g/L and 1.6g/L of biomass respectively, as compared to 0.3g/L and 0.16g/L obtained in the wastewaters alone revealing 700% and 900% increase respectively. Mixotrophic regimen cells as analyzed by a 2D Fourier transform infrared (FTIR) spectroscopy for its biochemical content revealed that fog's blended raw dairy waste (RDW) regimen cells had maximum

Glucose addition‐induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri

“…In the mixotrophic cultures, the content of protein in C. kessleri decreased from 54.3% to 34.9%, whereas that of carbohydrate increased from 12.6% to 28.2% with the increase of glucose concentrations from 2 to 15 g L −1 . In agreement with the present study, the same changes of protein and carbohydrate contents in C. pyrenoidosa were observed by Dhull et al, 46 who found that the contents of protein and carbohydrate were 65.0%, 20.3% and 39.3%, 54.0%, when C. pyrenoidosa grew in the photoautotrophic and mixotrophic cultures, respectively. Moreover, the content of lipids in C. kessleri increased firstly from 25.0% to 32.7% with increasing glucose concentrations from 2 to 6 g L −1 , and then maintained at c.32.0% when the glucose concentrations were > 6 g L −1 .…”

Investigation of autotrophic, heterotrophic, and mixotrophic modes of cultivation on lipid and biomass production in Chlorella vulgaris

“…Moreover, in another study, it has been revealed that Chlorella pyrenoidosa could be cultivated in mixotrophic mode using wastewaters to obtain a cost-effective and feasible alternative commercial medium for microalgal biomass production without the need for addition of an expensive organic carbon source to the culture medium. [22] On the other hand, heterotrophic mode of growth is regarded as the best cultivation strategy for lipid accumulation. [10] In the current study, we also observed the maximal lipid aggregation of 48.68% in heterotrophic metabolism.…”