Evaluation of atomic fluorescence spectrometry as a sensitive detection technique for arsenic speciation

Gómez‐Ariza, José Luis; Sánchez-Rodas, Daniel; Beltrán, Rafael; Corns, Warren T.; Stockwel, P.

doi:10.1002/(sici)1099-0739(199806)12:6<439::aid-aoc718>3.0.co;2-8

Cited by 74 publications

(26 citation statements)

References 17 publications

(13 reference statements)

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“…Extracted samples were then analysed by a hyphenated High Performance Liquid Chromatography (HPLC), Hydride Generation (HG) and Atomic Fluorescence Spectroscopy (AFS) system. HPLC separates the arsenic species on a strong anion exchange column (Gomez-Ariza et al, 1998;Moreno et al, 2000;Le et al, 2000). The coupled HG-AFS was obtained from PS Analytical.…”

Section: Laboratory Methodsmentioning

confidence: 99%

Characterisation of a mining-related arsenic-contaminated site, Cornwall, UK

Camm

Glass

Bryce³

et al. 2004

Journal of Geochemical Exploration

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Section: Laboratory Methodsmentioning

confidence: 99%

Characterisation of a mining-related arsenic-contaminated site, Cornwall, UK

Camm

Glass

Bryce³

et al. 2004

Journal of Geochemical Exploration

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“…However, the hydride generation-atomic fluorescence spectrometry (HG-AFS) coupled to HPLC represents a suitable alternative to this technique [22,23]. HG-AFS has been reported to be similar to inductively coupled plasma mass spectrometry (ICP-MS) regarding sensitivity and linear calibration range, although it has some advantages for arsenic speciation analysis, such as simplicity, lower acquisition and running costs [24][25][26]. However, the destruction of the organic part of arsenic species is required to determine them by HG-AFS, as they do not generate volatile hydrides or because the generation efficiency is very low [27].…”

Section: Introductionmentioning

confidence: 99%

Arsenic speciation in edible alga samples by microwave-assisted extraction and high performance liquid chromatography coupled to atomic fluorescence spectrometry

García-Salgado

Salgado

Bonilla

2012

Analytica Chimica Acta

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Keywords:Arsenic speciation Microwave-assisted extraction Edible algae Inductively coupled plasma atomic emission spectrometry High performance liquid chromatography Hydride generation-atomic fluorescence spectrometry Twelve commercially available edible marine algae from France, Japan and Spain and the certified reference material (CRM) NIES No. 9 Sargassum fulvellum were analyzed for total arsenic and arsenic species. Total arsenic concentrations were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) after microwave digestion and ranged from 23 to 126 (xgg -1 . Arsenic species in alga samples were extracted with deionized water by microwave-assisted extraction and showed extraction efficiencies from 49 to 98%, in terms of total arsenic. The presence of eleven arsenic species was studied by high performance liquid chromatography-ultraviolet photo-oxidation-hydride generation atomic-fluorescence spectrometry (HPLC-(UV)-HG-AFS) developed methods, using both anion and cation exchange chromatography. Glycerol and phosphate sugars were found in all alga samples analyzed, at concentrations between 0.11 and 22 (xgg -1 , whereas sulfonate and sulfate sugars were only detected in three of them (0.6-7.2 (xgg -1 ). Regarding arsenic toxic species, low concentration levels of dimethylarsinic acid (DMA) (<0.9 (xgg -1 ) and generally high arsenate (As(V)) concentrations (up to 77 (xgg -1 ) were found in most of the algae studied. The results obtained are of interest to highlight the need to perform speciation analysis and to introduce appropriate legislation to limit toxic arsenic species content in these food products.

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“…Arsenic metabolites in urine were analysed by high performance liquid chromatography with hydride generation and atomic Xuorescence spectrometry (HPLC-HG-AFS) according to Gomez-Ariza et al (1998) with minor changes. Samples were stored in plastic vials at ¡20°C.…”

Section: Hplc Analysis Of Arsenic Species In Urinementioning

confidence: 99%

Micronucleus frequency in peripheral blood lymphocytes and buccal mucosa cells of copper smelter workers, with special regard to arsenic exposure

Lewińska

Palus

Stępnik

et al. 2007

Int Arch Occup Environ Health

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Occupational exposure in copper smelters may produce various adverse health effects including cancer which, according to available epidemiologic data, is associated mainly with exposure to arsenic. Despite a number of well-documented studies reporting an increased risk of cancer among copper smelters workers, the data on genotoxic effects in this industry are scarce. In view of the above, an assessment of micronuclei (MN) frequency in peripheral blood lymphocytes and buccal epithelial cells from copper smelter workers was undertaken. Additionally, the clastogenic/aneugenic effect in lymphocytes was assessed with the fluorescence in situ hybridization (FISH). The study was conducted in three copper smelters in southwestern Poland. The subjects (n = 72) were enrolled among male workers at departments where As concentration in the air was up to at 80 microg/m(3). Exposure was assessed by measurement of arsenic concentration in urine and toenail samples. The control group (n = 83) was recruited from healthy male individuals living in central Poland who did not report any exposure to known genotoxins. The results of our study showed a significant increase in MN frequency in peripheral blood lymphocytes and in buccal epithelial cells of smelter workers, compared to the controls (7.96 +/- 4.28 vs. 3.47 +/- 1.70 and 0.98 +/- 0.76 vs. 0.50 +/- 0.52, respectively). The FISH technique revealed the presence of clastogenic and aneugenic effects in peripheral blood lymphocytes in both groups. The clastogenic effect was slightly more pronounced in the smelter workers; however, the difference was not statistically significant. The mean arsenic concentrations in urine (total arsenic species) and in toenail samples in the exposed group were 54.04 +/- 42.26 microg/l and 7.63 +/- 7.24 microg/g, respectively, being significantly different from control group 11.01 +/- 10.84 microg/l and 0.51 +/- 0.05 microg/g. No correlation between As content in urine or toenail samples and the genotoxic effect was found under study.

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Evaluation of atomic fluorescence spectrometry as a sensitive detection technique for arsenic speciation

Cited by 74 publications

References 17 publications

Characterisation of a mining-related arsenic-contaminated site, Cornwall, UK

Characterisation of a mining-related arsenic-contaminated site, Cornwall, UK

Arsenic speciation in edible alga samples by microwave-assisted extraction and high performance liquid chromatography coupled to atomic fluorescence spectrometry

Micronucleus frequency in peripheral blood lymphocytes and buccal mucosa cells of copper smelter workers, with special regard to arsenic exposure

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