Evaluation of assays for phenolic compounds on the basis of in vitro gas production by rumen micro-organisms

Wood, C.D.; Plumb, V. E.

doi:10.1016/0377-8401(95)00840-3

Cited by 14 publications

(7 citation statements)

References 20 publications

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“…33,34 Correlations between condensed tannin content and the initial rate of gas evolution were greater than with the pepsin-cellulase end-point digestibility method. Using a similar gas measurement technique, Wood and Plumb 35 have estimated a t max for phenolic inhibition of microbial fermentation of 9±12 h for tree leaves, which is well within the 8 h period in which the initial rates of gas evolution were determined in this study. The reduction in volatile fatty acid production, the reduced proportion of acetate and the increased levels of propionate for fermentations conducted in the presence of increasing condensed tannin concentration is in agreement with other in-vitro 36,37 and in-vivo 36 studies.…”

Section: Discussionsupporting

confidence: 58%

Responses ofLotus corniculatus to environmental change. 2. Effect of elevated CO2, temperature and drought on tissue digestion in relation to condensed tannin and carbohydrate accumulation

Carter

Theodorou

Morris

1999

J. Sci. Food Agric.

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Clonal plants of three genotypes of Lotus corniculatus (cv Leo) were grown in eight controlled environments under combinations of two temperature regimes, two CO2 concentrations and two watering regimes. Condensed tannins (proanthocyanidins), in‐vitro digestibility, initial rates of gas evolution (as an indicator of the initial rates of fermentation of the substrate), volatile fatty acid evolution, and non‐structural carbohydrate (NSC) levels were determined in leaves, stems and roots at full flowering. Under control conditions (average midsummer conditions in the United Kingdom) the total condensed tannin content of leaves varied six‐fold between genotypes but condensed tannin contents in stems and roots were similar. Condensed tannin levels were significantly increased in leaves and stems of all three genotypes by doubling the CO2 concentration while raising the temperature towards the optimum for growth significantly reduced condensed tannin levels. Drought stress significantly reduced condensed tannin levels in leaves and, particularly, in roots. Nutritive value was inversely related to condensed tannin levels in leaves and a negative relationship was observed between condensed tannin concentrations of more than 25–30 g kg−1 dry matter and the initial rates of gas evolution when subjected to in‐vitro fermentation with rumen micro‐organisms. In leaves, digestibility was significantly increased by drought and by increasing temperature but reduced by high CO2. In stems, digestibility was significantly increased by drought, but not significantly affected by increasing temperature, or by high CO2 alone. In roots, digestibility was significantly increased by drought, and decreased by increasing temperature or CO2. Increasing the growth temperature towards optimum growth reduced the content of NSC in all tissues with the greatest changes occurring in root tissue. Doubling the CO2 concentration increased NSC levels in leaves and stems with starch content more than doubled under high CO2 while, in roots, increased levels were only observed in combination with drought stress. There was a linear correlation between condensed tannin concentration and total NSC that was positive for leaves, neutral for stems and negative for roots. The relationship between carbohydrate levels and rates of gas production was negative for leaves and positive for stem and roots. © 1999 Society of Chemical Industry

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Section: Discussionsupporting

confidence: 58%

Responses ofLotus corniculatus to environmental change. 2. Effect of elevated CO2, temperature and drought on tissue digestion in relation to condensed tannin and carbohydrate accumulation

Carter

Theodorou

Morris

1999

J. Sci. Food Agric.

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“…There is only limited experimental evidence to indicate the usefulness of assays for phenols in nutritive value assessment. However, according to Wood and Plumb (1995), it may be suggested that TP has a similar effect on IVDMD as CT.…”

Section: Chemical Composition and Condensed Tannin Contentsmentioning

confidence: 99%

“…A major cause of low productivity of ruminant livestock in tropical countries is the inadequate amount and poor quality of feed. Tree leaves and shrubs are used as sources of fodder for ruminants in these regions, especially during the periods where both the quantity and quality of feed is limited ( Wood and Plumb , 1995). Despite their potential use as feed resources for ruminants, most tropical shrubs contain high levels of anti‐nutritional factors such as tannins ( Kumar and Singh , 1984).…”

Section: Introductionmentioning

confidence: 99%

In vitro quality assessment of two tropical shrub legumes in relation to their extractable tannins contents

Seresinhe

Iben

2003

Animal Physiology Nutrition

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The in vitro dry matter digestibility (IVDMD) of the two leguminous tree fodders Gliricidia sepium and Calliandra calothyrsus which differ in their tannin content was examined by the rumen simulation technique. Extractable condensed tannin (CT) concentrations ranged from 0.57% in G. sepium to 5.05% in C. calothyrsus using the butanol-HCl extraction. On the basis of their respective CT contents, G. sepium was classified as containing traces of CT, whereas C. calothyrsus had medium amounts of CT. Polyethylene glycol (PEG) was added at different concentrations (0, 1.5, 5 and 10 g/100 g plant material substrate) to assess the effect of tannins on IVDMD. The IVDMD was higher for G. sepium (range: 60-65%) than for C. calothyrsus (39.5-53.5%). In vitro gas production and IVDMD increased with increased PEG concentrations especially for C. calothyrsus. A non-significant response to increasing PEG concentrations for IVDMD of G. sepium confirms PEG binding with the tannins. On the basis of these results, it is assumed that G. sepium has a higher nutritive value than C. calothyrsus. Good relationship between PEG binding and the improvement of IVDMD confirms the usefulness of this technique for improving the nutritive value of tanniniferous tropical browses.

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“…The antinutrient substances (ANF) present in these feeds may also contribute to the reduction of the microbial activity (Wood and Plumb, 1995).…”

Section: Gas Productionmentioning

confidence: 99%

Chemical composition, organic matter digestibility and gas production of nine legume grains

Abreu¹,

Bruno-Soares²

1998

Animal Feed Science and Technology

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Nine legume grains (Pisum satiuum, Cicer arietinum, Vicia satiua, Vicia faba, Lathyrus ochrus, Lathyrus &era, Lupinus angustifolius and Lupinus luteus) were studied with respect to their chemical composition, organic matter digestibility (OMD) and digestible energy (DE) using rams in metabolic cages. Gas production by grains was evaluated using rumen inoculum. The majority of these legume grains showed values of: crude protein (CP) -21 to 24%, ether extract (EE) -1.5 to 5%, neutral detergent fibre (NDF) -15 to 22%, acid detergent fibre (ADF) -6 to 13%, starch -30 to 40%, OMD -90 to 92%, DE -16 to 17 MJ kg-' dry matter (DM) and gas production (48 h) of 110 to 145 ml g-' DM. Lupinus however showed much higher values of CP (29.1% and 34.4%), NDF (26.9% and 28.6%), ADF (22.9% and 24.6%), EE (5.4% and 7.2%) and lower values of starch about 0.8%. OMD (83.6% and 86.0%) and gas production (95.9 and 104.6 ml g-' DM). Gas production (48 h) was negatively correlated to CP (r = -0.74; P < 0.05), NDF (r = -0.93; P < O.OOl>, ADF (r = -0.78; P < O.Ol), positively with starch (r = 0.70; P < 0.05) and positively with OMD (r = 0.68; P < 0.05). Gas production in this type of feed was not shown to be a good criterion for assessing their OMD. 0 1998 Elsevier Science B.V.

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Evaluation of assays for phenolic compounds on the basis of in vitro gas production by rumen micro-organisms

Cited by 14 publications

References 20 publications

Responses ofLotus corniculatus to environmental change. 2. Effect of elevated CO2, temperature and drought on tissue digestion in relation to condensed tannin and carbohydrate accumulation

Responses ofLotus corniculatus to environmental change. 2. Effect of elevated CO2, temperature and drought on tissue digestion in relation to condensed tannin and carbohydrate accumulation

In vitro quality assessment of two tropical shrub legumes in relation to their extractable tannins contents

Chemical composition, organic matter digestibility and gas production of nine legume grains

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