Evaluation of artificial skin (Integra) in a rodent model

King, Walter W. K.; Lam, Ping Keun; Liew, C. T.; Ho, Weiguang; Lil, A.K.C

doi:10.1016/s0305-4179(97)90098-x

Cited by 28 publications

(16 citation statements)

References 6 publications

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“…For example, commercial products as Alloderm® (LifeCell Corporation, Branchburg, NJ), Oasis® (Healthpoint Ltd., Fort Worth, TX), Integra® Dermal Regeneration Template (Integra Life Sciences Holding Co., South Plainfield, NJ) and Biobrane® (Bertek Pharmaceuticals, Sugarland, TX) are generally included in the skin substitute category; these have been reviewed elsewhere 2–7. The Integra dermal regeneration matrix consists of a collagen/glycosaminoglycan mixture fabricated into a crosslinked sponge and covered with a silicone elastic membrane 4, 15, 16. Other matrices have been prepared from decellularized tissues, such as porcine small intestinal submucosa17–21 or human donor dermis 22.…”

Section: Introductionmentioning

confidence: 99%

Haptide‐coated collagen sponge as a bioactive matrix for tissue regeneration

Marx¹,

Hotovely-Salomon²,

Levdansky

et al. 2007

J Biomed Mater Res

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We previously described a new class of conserved, cell adhesive (haptotactic) peptides, termed Haptides, based on sequences first identified in fibrinogen. Here, we describe a new biomaterial, Haptide-coated Collagen, in which the carbodiimide reagent, EDC, was used to covalently couple a Haptide (preC gamma), equivalent to the carboxy terminus of the fibrinogen gamma chain, to a cross-linked sponge composed of bovine collagen type I. The dose response of Haptide bound to collagen on cell attachment response reached a plateau at a concentration of 5-10 mg Haptide/g collagen. The Haptized-collagen was more stable to 1N NaOH, with a degradation half-time (T(1/2)) of 1.7 h, compared to 0.9 h for untreated control. Haptized collagen discs could be loaded with approximately 30% more human dermal fibroblasts or bovine aortic endothelial cells than unmodified collagen discs (p < 0.001). After a proliferation phase, Haptized collagen discs contained approximately 45% more fibroblasts than non-Haptized discs (p < 0.01). Histological analysis following sub-dermal implantation in rats indicated that at day 8, Haptized collagen sponge was less degraded than unmodified collagen sponge, attracted more endogenous fibroblasts with newly deposited collagen, and provoked less inflammatory or other adverse reactions. These results suggest potential clinical applications for Haptized collagen sponge for tissue regeneration, soft tissue augmentation, skin repair, and wound healing.

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Section: Introductionmentioning

confidence: 99%

Haptide‐coated collagen sponge as a bioactive matrix for tissue regeneration

Marx¹,

Hotovely-Salomon²,

Levdansky

et al. 2007

J Biomed Mater Res

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“…King et al . had investigated the biocompatibility of Integra in the clean surgical wounds of 20 guinea pigs and proved that Integra is readily biologically incorporated into surrounding viable tissue [106]. Baker and Iorio effectively reversed the pathology of a chronic wound of type II diabetes by the application of an Apligraf skin graft substitute along with autologous platelet-derived growth factor [107].…”

Section: Applications Of Protein Materialsmentioning

confidence: 99%

Applications and Degradation of Proteins Used as Tissue Engineering Materials

Wang

Ren

et al. 2009

Materials

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This article provides an up-to-date review on the applications of natural polymers, i.e., proteins, as materials for tissue engineering. Proteins are one of the important candidates for tissue engineering materials based on their superior biocompatibility, biodegradation, bioresorbability, and so on. However, their inferior mechanical properties limit their broad application. Currently-available proteins for application in tissue engineering or drug delivery systems, such as fibrin, collagen, zein, silk fibroin, keratin, casein and albumin, and the biodegradation of tissue-engineered substitutes based on proteins are presented. Techniques of scaffold fabrication are also mentioned. Problems and future possibilities for development of protein-based tissue-engineered substitutes are also introduced in this review.

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“…10 Therefore, further developments that facilitate the healing process or address issues such as the control of the chemical environment and bacterial infection are required and desired. Indiscriminately, synthetic, [11][12][13][14] natural, 8,15,16 or biological materials 10,17,18 have been presented along the years, as the key elements for controlling/modulating the healing mechanisms and the outcomes of wound repair upon dressing. 10 Among the recently proposed natural-origin materials for wound dressing, collagen 2,9,19 chitosan, [20][21][22][23][24] and silk 25,26 occupy a central position due to their nature, which might present improved performance.…”

Section: Introductionmentioning

confidence: 99%

“…Among the many different animal models to study wound healing and skin regeneration, excision of rat skin portions is a model widely used. 8,17,22,[40][41][42][43][44][45] Therefore, a wound-dressing rat model of partial-thickness skin wound, under impaired healing conditions, was used to assess the suitability of the cht/soy membranes to promote wound healing. Comparatively, Epigard Ò , a clinically accepted wound dresser, was used as control.…”

Section: Introductionmentioning

confidence: 99%

In VivoPerformance of Chitosan/Soy-Based Membranes as Wound-Dressing Devices for Acute Skin Wounds

Santos

Höring

Reise

et al. 2013

Tissue Engineering Part A

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Wound management represents a major clinical challenge on what concerns healing enhancement and pain control. The selection of an appropriate dressing plays an important role in both recovery and esthetic appearance of the regenerated tissue. Despite the wide range of available dressings, the progress in the wound care market relies on the increasing interest in using natural-based biomedical products. Herein, a rat wounddressing model of partial-thickness skin wounds was used to study newly developed chitosan/soy (cht/soy)-based membranes as wound-dressing materials. Healing and repair of nondressed, cht/soy membrane-dressed, and Epigard Ò -dressed wounds were followed macroscopically and histologically for 1 and 2 weeks. cht/soy membranes performed better than the controls, promoting a faster wound repair. Re-epithelialization, observed 1 week after wounding, was followed by cornification of the outermost epidermal layer at the second week of dressing, indicating repair of the wounded tissue. The use of this rodent model, although in impaired healing conditions, may enclose some drawbacks regarding the inevitable wound contraction. Moreover, being the main purpose the evaluation of cht/soy-based membranes' performance in the absence of growth factors, the choice of a clinically relevant positive control was limited to a polymeric mesh, without any growth factor influencing skin healing/repair, Epigard. These new cht/soy membranes possess the desired features regarding healing/repair stimulation, ease of handling, and final esthetic appearance-thus, valuable properties for wound dressings.

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Evaluation of artificial skin (Integra) in a rodent model

Cited by 28 publications

References 6 publications

Haptide‐coated collagen sponge as a bioactive matrix for tissue regeneration

Haptide‐coated collagen sponge as a bioactive matrix for tissue regeneration

Applications and Degradation of Proteins Used as Tissue Engineering Materials

In VivoPerformance of Chitosan/Soy-Based Membranes as Wound-Dressing Devices for Acute Skin Wounds

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