Evaluation of apple varieties of the Sverdlovsk horticultural breeding station according to the ethylene biosynthesis genes using molecular markers

Shamshin, I. N.; Тележинский, Д. Д.; Шлявас, А. В.

doi:10.30766/2072-9081.2020.21.6.706-712

Cited by 4 publications

(2 citation statements)

References 19 publications

(28 reference statements)

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“…В его геноме имеются аллели Md-ACO1-1 и Md-ACS1-2, что позволяет его использовать в селекции на длительную лежкость плодов. Сочетание этих аллелей в гомозиготном состоянии в одном генотипе снижает выработку этилена в плодах, что способствует их длительному сроку хранения [10]. Морфологическое описание сорта Аксена.…”

Section: янтарка алтайскаяunclassified

Morpho-biological and economically valuable traits of a new apple variety Aksyona

Telezhinskiy¹

2023

asj

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The breeders of the Sverdlovsk breeding station of horticulture created a new apple variety Aksyona, which was included in the State Register of Breeding Achievements of the Russian Federation, approved for use on the territory of the Russian Federation in the Volga-Vyatka region in 2022. The parents of this variety are the Ural summer variety Serebryanoye Kopyttse and the donor of immunity to scab 22-40-67, created at Russian Research Institute of Fruit Crop Breeding. Hybridization was carried out in 1993. Studies of the new variety were carried out in 2001 - 2021 on the territory of the Sverdlovsk breeding station of horticulture . The average yield for 4 years of testing the Aksyona variety on the clonal rootstock 62-396 was 226.6 q/ha. This variety is immune to scab (Rvi6 gene), all three QTL FBF7 markers are present in its genotype, which suggests its resistance to fire blight disease. Variety Aksyona is heterozygous for two genes Md-ACO1 and Md-ACS1 and is of interest as an initial form in breeding for a low level of endogenous ethylene biosynthesis, which affects the duration of fruit storage. This variety showed high winter hardiness over the years of testing, has fruits of medium size (110 g), one-dimensional, round, regular shape, with a smooth surface, attractive appearance. The main color is light yellow, the integumentary coloration of the fruit is orange-red, blurry-striped. The skin is dry, matte. The pulp is creamy, medium density, fine-grained, juicy, good sweet and sour taste, with aroma. The ripening period is early summer, the period of consumption is 25 days. The tree is medium-sized, with a rounded crown of medium density, skeletal branches depart from the trunk at an angle close to a right one. The variety is early-growing, comes into fruiting in the fourth year after grafting. Authors of the variety: L.A. Kotov, T.A. Makarova.

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Section: янтарка алтайскаяunclassified

Morpho-biological and economically valuable traits of a new apple variety Aksyona

Telezhinskiy¹

2023

asj

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“…Стоит отметить, что в россии� скои� генплазме яблони селекционно ценныи� аллель Md-ACS1-2 в гомозиготном состоянии встречается очень редко. При этом его комбинация с геном Rvi-6 не выявлена (Shamshin et al, 2020;Suprun, Tokmakov, 2013).…”

Section: Introductionunclassified

Marker-assisted selection in the development of advanced apple-tree forms and donors combining scab resistance with increased fruit storability

Suprun,

Egorov,

Nasonov

et al. 2023

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Cultivation of scab-resistant apple cultivars with better fruit storability increases the economic efficiency and environmental friendliness of horticulture. Hence, the development of such cultivars is an important task in modern apple-tree breeding.Materials and methods. A set of 646 hybrid plants obtained in six cross combinations (Renet Simirenko/Modi, Renet Simirenko/Smeralda, Renet Simirenko/Renoir, Renet Simirenko/Fujion, Renoir/Granny Smith, and Modi/Granny Smith) was studied. Their scab resistance was assessed under natural infection pressure for two years using a quantitative scale. The Rvi6 gene was identified using the PCR analysis with the primer pair VfC1+VfC. The Md-ACS1 gene alleles were detected with a known SCAR DNA marker. The chi-square test was applied for statistical confirmation of the data.Results. Based on the DNA marker analysis, 328 plants carrying the dominant allele of the Rvi6 gene were identified. The results of the phenotypic resistance assessment confirmed the correspondence of the resistant phenotype / dominant allele of the gene for most plants. Molecular genetics analysis of the Md-ACS1 gene disclosed its allelic combinations. A total of 190, 322, and 126 plants carrying allelic variants of Md-ACS1-2/2, Md-ACS1-1/2, and Md-ACS1-1/1, respectively, were identified. Among the plants with the dominant allele of the scab resistance Rvi6 gene, 92 plant forms were identified with Md-ACS1-2/2, and 143 heterozygous ones (Md-ACS1-1/2), also valuable for breeders.Conclusion. A wide range of apple breeding forms carrying valuable alleles of the Rvi6 and Md-ACS1 genes were selected. Hybrid forms with target alleles of both genes responsible for important agronomic traits would contribute to the intensification of breeding efforts aimed at producing scab-resistant apple cultivars with increased fruit storability. They can also be used for breeding purposes as complex donors.

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Identification of apple genes <i>Md-Exp7</i> and <i>Md-PG1</i> alleles in advanced selections resistant to scab

et al. 2022

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The creation of apple varieties with a high level of f lesh f irmness and long shelf life is one of the important goals in breeding. Among the genes controlling these traits, the role of the endogenous ethylene biosynthesis control gene, Md-ACS1, the expansin gene, Md-Exp7, and the polygalacturonase gene, Md-PG1, has been established. The use of DNA marker analysis to solve problems in breeding for fruit quality traits allows one not only to track several target genes simultaneously, but also to cull plants with undesirable alleles at the early stages of development. In order to select complex donors of breeding traits, molecular genetic identif ication of the genes that determine the quality traits of apple fruits Md-Exp7 and Md-PG1 was performed in 256 breeding selections carrying the scab resistance gene Rvi6 and valuable allelic variants of the Md-ACS-1 gene, which determines the endogenous synthesis of ethylene in fruits: 90 samples with the Md-ACS1 allele (2/2) and 166 samples with Md-ACS1 (1/2). As a result of the study, an allelic combination for the Md-Exp7 and Md-PG1 genes was established. Analysis of the parental cultivars (Renet Simirenko, Modi, Smeralda, Renoir, Fulzhion and Granny Smith) used to obtain hybrid selections revealed three alleles 198, 202, 214 bp according to the DNA marker of the Md-Exp7 gene. The SSR marker for the Md-PG1 gene amplif ied three alleles (289, 292, 298 bp) on the abovementioned cultivars. Within the 256 breeding selections samples that have the most priority for breeding alleles of the desired genes in combination with the Rvi6 gene and/or with selection-priority allelic variants of the Md-ACS-1 gene were identif ied. Of the most valuable for breeding, 46 accessions carrying the combination Md-Exp7 (202:202) + Md-ACS1 (2/2) were distinguished. Hybrids with alleles Md-PG1 (292:292) + Md-ACS1 (2/2) are also most valuable for use in breeding and as donors of selection-valuable alleles; 21 samples were identif ied. Accessions with a complex of breeding-valuable target alleles are valuable complex donors, as well as valuable breeding material for creating varieties with improved fruit quality characteristics and scab resistance.

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Evaluation of apple varieties of the Sverdlovsk horticultural breeding station according to the ethylene biosynthesis genes using molecular markers

Cited by 4 publications

References 19 publications

Morpho-biological and economically valuable traits of a new apple variety Aksyona

Morpho-biological and economically valuable traits of a new apple variety Aksyona

Marker-assisted selection in the development of advanced apple-tree forms and donors combining scab resistance with increased fruit storability

Identification of apple genes <i>Md-Exp7</i> and <i>Md-PG1</i> alleles in advanced selections resistant to scab

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