Evaluation of apoptosis of tumor and of apparently normal cells in human renal carcinoma

Roman, Stela; Petrusca, Daniela N.; Moldovan, Ioana; Paraoan, Marius; Petrescu, Amelia; Damian, Daniel; Noica, Niculae; Sulica, Andrei

doi:10.1016/s0165-2478(98)00110-2

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…TILs were isolated from tumor tissues by enzymatic digestion as previously described (18). In brief, after surgical resection, the tissues maintained under sterile conditions were finely minced and enzymatically digested in RPMI 1640 U/mL hyaluronidase V, 30 U/mL DNase I, 300 U/mL collagenase type IV (all from Sigma), and antibiotics (100 U/mL penicillin, 100 µg/mL streptomycin, and 100 µg/mL gentamycin, all from Life Technologies, USA).…”

Section: Methodsmentioning

confidence: 99%

Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro

Yang

Qian

et al. 2012

Braz J Med Biol Res

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Thymosin alpha 1 (Tα1) has been shown to have beneficial effects on numerous immune system parameters, but little is known about the effects of Tα1 on patients with gastric carcinoma. The objective of this study was to determine the effect of Tα1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro, and to evaluate its efficacy as an immunoregulatory factor in patients with gastric carcinoma. We compared the effect of Tα1 on the frequency of CD4+ and CD8+ T cells, especially the CD4+CD25+Foxp3+ Tregs in peripheral blood mononuclear cells (PBMCs) from gastric carcinoma patients (N = 35) and healthy donors (N = 22). We also analyzed the changes in the proliferation of PBMCs in response to treatment with Tα1, and examined the production of Th1, Th2, and Th17 cytokines by PBMCs and tumor-infiltrating lymphocytes. The treatment of PBMCs from gastric cancer patients, with Tα1 (50 µg/mL) alone increased the percentage of CD4+CD25+Foxp3+ (suppressive antitumor-specific Tregs) from 1.68 ± 0.697 to 2.19 ± 0.795% (P < 0.05). Our results indicate that Tα1 increases the percentage of Tregs and IL-1β, TNF-α, and IL-6 in vitro.

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Section: Methodsmentioning

confidence: 99%

Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro

Yang

Qian

et al. 2012

Braz J Med Biol Res

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“…14 Apoptotic cells with mean percentage of 22±13 in TCC of bladder was reported by Roman et al 15 In two other studies the frequency of apoptosis using TUNEL technique in TCC of the renal pelvis and ureter has been studied, 16,17 and a close association of the incidence of apoptosis with the tumor grade of TCC was observed, indicating that apoptotic cell death may play a role in the tumor progression of renal pelvic and ureteral TCC.…”

Section: Discussionmentioning

confidence: 81%

Apoptosis in transitional cell carcinoma of bladder and its relation to proliferation and expression of P53 and Bcl-2

Amirghofran

Monabati

Khezri

et al. 2004

Pathol. Oncol. Res.

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Apoptosis-detecting radioligands: current state of the art and future perspectives

Lahorte

Vanderheyden²,

Steinmetz³

et al. 2004

Eur J Nucl Med Mol Imaging

181

132

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This review provides a critical and thorough overview of the radiopharmaceutical development and in vivo evaluation of all apoptosis-detecting radioligands that have emerged so far, along with their possible applications in nuclear medicine. The following SPECT and PET radioligands are discussed: all forms of halogenated Annexin V (i.e. (123)I-labelled, (124)I-labelled, (125)I-labelled, (18)F-labelled), (99m)Tc/(94m)Tc-labelled Annexin V derivatives using different chelators and co-ligands (i.e. BTAP, Hynic, iminothiolane, MAG(3), EDDA, EC, tricarbonyl, SDH) or direct (99m)Tc-labelling, (99m)Tc-labelled Annexin V mutants and (99m)Tc/(18)F-radiopeptide constructs (i.e. AFIM molecules), (111)In-DTPA-PEG-Annexin V, (11)C-Annexin V and (64)Cu-, (67)Ga- and (68)Ga-DOTA-Annexin V. In addition, the potential role and clinical relevance of anti-PS monoclonal antibodies and other alternative apoptosis markers are reviewed, including: anti-Annexin V monoclonal antibodies, radiolabelled caspase inhibitors and substrates and mitochondrial membrane permeability targeting radioligands. Nevertheless, major emphasis is placed on the group of Annexin V-based radioligands, in particular (99m)Tc-Hynic-Annexin V, since this molecule is by far the most extensively investigated and best-characterised apoptosis marker at present. Furthermore, the newly emerging imaging modalities for in vivo detection of programmed cell death, such as MRI, MRS, optical, bioluminescent and ultrasound imaging, are briefly described. Finally, some future perspectives are presented with the aim of promoting the development of potential new strategies in pursuit of the ideal cell death-detecting radioligand.

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Evaluation of apoptosis of tumor and of apparently normal cells in human renal carcinoma

Cited by 4 publications

References 24 publications

Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro

Effect of thymosin alpha-1 on subpopulations of Th1, Th2, Th17, and regulatory T cells (Tregs) in vitro

Apoptosis in transitional cell carcinoma of bladder and its relation to proliferation and expression of P53 and Bcl-2

Apoptosis-detecting radioligands: current state of the art and future perspectives

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