Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

Oliveira, Sandro de; Souza, Glalci Alves de; Eckert, Camila Rodrigues; Silva, Thuany Alves; Sobral, Edmar Silva; Fávero, Oriana A.; Ferreira, Marcelo J. P.; Romoff, Paulete; Baader, W. J.

doi:10.5935/0100-4042.20140076

Cited by 31 publications

(31 citation statements)

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“…The finding can be explained by the difference in the sensitivity of the assays. It has been found that the DPPH radical reacts only with more reactive antioxidants as was found by de Olivera et al The reducing power of the PFCs studied was confirmed in the TAC assay, that once again showed the importance of both the CF 3 and OCH 3 groups in the benzene ring of the R substituent for capacity of the PF compounds to exhibit the antioxidant potency. We found that the reducing abilities of the PFCs evaluated were much weaker than trolox used as standard antiradical compound.…”

Section: Resultssupporting

confidence: 68%

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

et al. 2017

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Flavones exhibit a variety of beneficial effects and are well known for their medicinal importance in several diseases, including cardiovascular, neurodegenerative and cancer. The inclusion of the piperazine ring to the flavone backbone is an important strategy in drug discovery but only a few studies have synthesized piperazinyl flavone compounds to test their biological activity. While there is a major focus on the antioxidant properties of drugs in therapy of several diseases of inflammatory origin, we synthesized a series of the novel piperazinyl flavone analogues bearing the phenyl ring with different substituents. The analogues were evaluated for in vitro antioxidant activity against superoxide anion radical, hydroxyl radical, 2,2-diphenyl-1-picrylhydrazyl radical, and hydrogen peroxide scavenging properties. The total antioxidant status based on the absorbance of the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation (ABTS ) and total antioxidant capacity using the Fe(III)-ferrozine complex were also monitored. The results of the above studies showed that the compounds synthesized were found possessed moderate radical scavenging potential, and that their interaction with reactive oxygen species is complex and depends on their structural conformation and the type of substituent R in the piperazine ring being attached. Best antiradical activity were found for the compounds with methoxy groups on the phenyl ring of substituent R, whereas the presence of methoxy or trifluoromethyl groups in substituent R resulted in higher ABTS and ion Fe(III) reduction. These compounds are promising molecules to be used for their antioxidant properties and may be regarded, after improvement of the antioxidant potential, to control diseases of free radical etiology.

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Section: Resultssupporting

confidence: 68%

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

et al. 2017

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“…The obtained %Tx was 32 � 1% showing that the phases of M. floribundum were more active. [22] Analyzing the isolated compounds, flavones (1, 2) presented a low activity, less than 32 % Tx activity. However, chlorogenic acid derivatives (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) were more active than flavones showing higher ability to trapping DPPH radicals.…”

Section: Antiradical Assaymentioning

confidence: 99%

Caffeoylquinic Acids: Separation Method, Antiradical Properties and Cytotoxicity

Tamayose

Santos

Roque

et al. 2019

Chemistry & Biodiversity

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Twelve chlorogenic acid derivatives and two flavones were isolated from Moquiniastrum floribundum (Asteraceae, other name: Gochnatia floribunda). Compounds were evaluated in relation to their cytotoxicity and antiradical properties. Cytotoxicity was not observed for compounds, however, chlorogenic acid derivatives showed antiradical activity and were more active than the Trolox standard. Quinic acid esterified with caffeoyl group at C‐4 position showed higher antiradical activity compared to acylation at C‐3 or C‐5 positions. Additional caffeoyl groups esterified in quinic acid increase the antiradical activity observed for 4‐caffeoylquinic acid. Excepted to 3,4‐dicaffeoylquinic acid methyl ester, methyl ester derivatives show higher capacity of trapping radicals than their respective acids. Consequently, the presence of caffeoyl group at C‐4 position of quinic acid is suggested as fundamental to obtain the highest antiradical activity.

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“…where I 0 is the maximum CL intensity for standard and I is the maximum CL intensity for sample at t = 5 s after reaction initiation [28]. A calibration curve was constructed using Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic Acid, ≥98%, Merck KGaA, Germany) in the concentration range 8-168 M and the results were expressed as M Trolox equivalence [27].…”

Section: Evaluation Of Antioxidant and Antifungal Activitymentioning

confidence: 99%

Innovative phytosynthesized silver nanoarchitectures with enhanced antifungal and antioxidant properties

Orţan

Fierăscu

Ungureanu

et al. 2015

Applied Surface Science

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Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

Cited by 31 publications

References 1 publication

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

Synthesis and in vitro antioxidant activity study of some new piperazinyl flavone compounds

Caffeoylquinic Acids: Separation Method, Antiradical Properties and Cytotoxicity

Innovative phytosynthesized silver nanoarchitectures with enhanced antifungal and antioxidant properties

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