Evaluation of Antiproliferative Activity of Red Sorghum Bran Anthocyanin on a Human Breast Cancer Cell Line (MCF-7)

Devi, Poornima; Kumar, M. Saravana; Das, Sudipta

doi:10.4061/2011/891481

Cited by 48 publications

(27 citation statements)

References 15 publications

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“…In addition, the present study suggested that luteolin suppressed MdS cell proliferation, mainly as a result of the induction of apoptosis, as demonstrated by typical morphological features of cell apoptosis, including cell shrinkage, chromatin condensation and loss of normal nuclear architecture, under the microscope using Wright-Giemsa staining. The formation of dNA fragments of oligonucleosomal size (180-200 bp) is considered to be a hallmark of apoptotic cell death in several cell types (29). In the present study, the agarose gel electrophoresis showed a typical ladder pattern of genomic dNA fragmentation in the nuclei isolated from SKM-1 cells (Fig.…”

Section: Discussionsupporting

confidence: 64%

Luteolin induces myelodysplastic syndrome‑derived cell apoptosis via the p53‑dependent mitochondrial signaling pathway mediated by reactive oxygen species

et al. 2018

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Luteolin, a common dietary flavonoid, induces the apoptosis of cells in several types of cancer. However, its role in myelodysplastic syndrome (MDS) and the potential underlying mechanisms remain to be elucidated. To evaluate the potential benefit and underlying mechanisms of luteolin in MDS cells, the viability of SKM‑1 cells and primary bone marrow (PBM) mononuclear cells from patients with intermediate‑ or high‑risk MDS were assessed using a Cell Counting Kit‑8 assay. The apoptotic features of cell morphology were assessed using Wright‑Giemsa staining, DNA fragmentation was analyzed by agarose gel electrophoresis, and the extent of apoptosis was quantified by flow cytometry (FCM). Reactive oxygen species (ROS) were measured by FCM with 2,7‑dichlorodihydrofluorescein diacetate staining and mitochondrial membrane potential (ΔΨm) was determined using 5,5',6,6'‑tetrachloro‑1,1',3,3'‑tetraethylbenzimidazolylcarbocyanine iodide staining. Caspase activity was detected using a fluorometric protease assay. Furthermore, the effects of luteolin on the expression of apoptosis‑related proteins were analyzed using western blot analysis. The resulting data revealed that luteolin significantly inhibited the proliferation of SKM‑1 cells in vitro, and its half maximal inhibitory concentration was 139.41 µM at 24 h and 23.95 µM at 72 h. Luteolin also markedly inhibited the proliferation of mononuclear cells from patients with intermediate‑ or high‑risk MDS. Luteolin suppressed cell proliferation, mainly as a result of the induction of apoptosis, as demonstrated by typical apoptotic morphological features, the ladder pattern of genomic DNA fragmentation, and the results of FCM using Annexin V‑FITC/PI double staining. It was also found that short‑term exposure of SKM‑1 cells to luteolin led to a marked increase in the accumulation of ROS. The increased intracellular level of ROS appeared to induce the activation of p53 and elevate the B‑cell lymphoma 2 (Bcl‑2)‑associated X protein/Bcl‑2 ratio, which modulates ΔΨm and triggers the release of cytochrome c, and may increase the activities of apoptotic protease activating factor 1, caspase‑3, ‑8 and ‑9 to further trigger the destruction of structural and specific proteins and thereby cell apoptosis. Notably, the inhibition of ROS generation by the antioxidant N‑acetyl‑L‑cysteine significantly attenuated the luteolin‑induced loss of ΔΨm and activities of caspase‑3, ‑8 and ‑9. These data suggested that luteolin exerts its pro‑apoptotic action partly through the p53‑dependent mitochondrial signaling pathway mediated by intracellular ROS, which provides a promising therapeutic candidate for patients with MDS.

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Section: Discussionsupporting

confidence: 64%

Luteolin induces myelodysplastic syndrome‑derived cell apoptosis via the p53‑dependent mitochondrial signaling pathway mediated by reactive oxygen species

et al. 2018

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“…These events may disrupt the cellular physiology thus leading to cell death. This type of cell morphology disruption of HCT-15 cells has earlier been observed with treatment of various other compounds [38][39][40] . …”

Section: Resultssupporting

confidence: 57%

Mechanistic Studies of in vitroAnti-Proliferative Potential of Arisaena intermedium Lectin

Gautam¹,

Kaur²,

Thakur³

et al. 2016

IJLSSR

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ABSTRACT-Aberrant glycosylation has been recognized as hallmark of cancer. Exploiting differences in glycosylation between malignant and healthy tissues offers excellent opportunities to identify sensitive and specific cancer biomarkers. Plant lectins have demonstrated the ability to specifically agglutinate malignant transformed cells. Lectins are sugar binding proteins or glycoprotein of non-immune origin which agglutinate cells or precipitate glycol-conjugates. Some lectins shown to the anti-proliferative effect on cancer cells. A wide scope of this application of lectins is that it can be used for diagnosis as well as therapeutics of cancer. The objective of the present study was to purify a lectin from tubers of Arisaema intermedium and evaluate in vitro anti-proliferative potential towards HCT-15, a human colon cancer cell line. The present study was conceived as an offshoot to the ongoing work on lectins in our laboratory. The already reported Arisaema intermedium (AIL) lectin was purified on asialofetuin linked amino-activated silica bead matrix. The purity of the affinity purified lectin was ascertained by SDS-PAGE, pH-8.3. The lectin activity was assessed by hemagglutination and protein concentration was determined by Lowry's method. The cytotoxicity of AIL towards HCT-15 was evaluated by MTT assay. The mechanism of anti-proliferative effect was assessed by evaluation of cell morphology, trypan blue exclusion assay, DNA fragmentation and nucleic acid content determination.

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“…In black sorghum genotypes, the predominant flavonoids are the 3-deoxyanthocyanidins. These compounds are unique and have potential use as a natural colorant in low pH food systems as well as anti-inflammatory and anti-cancer activity (Awika et al, 2004;Burdette et al, 2010;Devi et al, 2011;Shih et al, 2007;Yang et al, 2009). The 3-deoxyanthocyanidins are present in non-methoxylated and methoxylated forms.…”

Section: Introductionmentioning

confidence: 99%

Effect of genotype and environment on flavonoid concentration and profile of black sorghum grains

Taleon

Dykes

Rooney

et al. 2012

Journal of Cereal Science

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Evaluation of Antiproliferative Activity of Red Sorghum Bran Anthocyanin on a Human Breast Cancer Cell Line (MCF-7)

Cited by 48 publications

References 15 publications

Luteolin induces myelodysplastic syndrome‑derived cell apoptosis via the p53‑dependent mitochondrial signaling pathway mediated by reactive oxygen species

Luteolin induces myelodysplastic syndrome‑derived cell apoptosis via the p53‑dependent mitochondrial signaling pathway mediated by reactive oxygen species

Mechanistic Studies of in vitroAnti-Proliferative Potential of Arisaena intermedium Lectin

Effect of genotype and environment on flavonoid concentration and profile of black sorghum grains

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