Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116

Obradović, Ana; Žižić, Jovana B.; Trišović, Nemanja; Božić, Bojan; Ušćumlić, Gordana S.; Božić, Biljana; Marković, Snežana D.

doi:10.3906/biy-1302-15

Cited by 11 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite the fact that these patients are treated with standard chemotherapeutic medicines, their prognosis is poor, with a 5-year survival rate of only 15% [ 4 ]. Due to the development of cellular resistance, traditional chemotherapy has been ineffective in cancer treatment [ 5 , 6 ]. Furthermore, traditional chemotherapy and advanced molecular drug therapy are both expensive and inconvenient for patients [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation

et al. 2023

View full text Add to dashboard Cite

The goal of current research was to develop a new form of effective drug, curcumin-loaded solid lipid nanoparticles (Cur-SLNs) and test its efficacy in the treatment of lung cancer. Different batches of SLNs were prepared by the emulsification–ultrasonication method. For the optimization of formulation, each batch was evaluated for particle size, polydispersity index (PI), zeta potential (ZP), entrapment efficiency (EE) and drug loading (DL). The formulation components and process parameters largely affected the quality of SLNs. The SLNs obtained with particle size, 114.9 ± 1.36 nm; PI, 0.112 ± 0.005; ZP, −32.3 ± 0.30 mV; EE, 69.74 ± 2.03%, and DL, 0.81 ± 0.04% was designated as an optimized formulation. The formulation was freeze-dried to remove excess water to improve the physical stability. Freeze-dried Cur-SLNs showed 99.32% of drug release and demonstrated a burst effect trailed by sustained release up to 120 h periods. The erythrocyte toxicity study of Cur-SLNs and its components demonstrated moderate hemolytic potential towards red blood cells (RBCs). The cytotoxic potential of the formulation and plain curcumin was estimated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against A549 cell line. After 48 h of incubation, Cur-SLNs demonstrated more cytotoxicity (IC50 = 26.12 ± 1.24 µM) than plain curcumin (IC50 = 35.12 ± 2.33 µM). Moreover, the cellular uptake of curcumin was found to be significantly higher from Cur-SLNs (682.08 ± 6.33 ng/μg) compared to plain curcumin (162.4 ± 4.2 ng/μg). Additionally, the optimized formulation was found to be stable over the period of 90 days of storage. Hence, curcumin-loaded SLNs can be prepared using the proposed cost effective method, and can be utilized as an effective drug delivery system for the treatment of lung cancer, provided in vivo studies warrant a similar outcome.

show abstract

Section: Introductionmentioning

confidence: 99%

Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Lung cancer has become one of the common diseases worldwide that in terms of the cause of death of the patients (Duan and Zhang, 2006). Chemotherapy is an effective approach to cancer therapy including lung cancer, but it is limited since the developing resistance towards current chemotherapeutics like vinblastine and paclitaxel has been reported frequently (Spitz et al, 2009;Hsieh et al, 2010;Obradovic et al, 2013). This fact indicates a need for novel chemotherapy agents that are effective in low concentrations and short time application.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells

Kandemir

ÇELİKOĞLU

KANDEMİR

et al. 2022

Black Sea Journal of Health Science

View full text Add to dashboard Cite

Iris taochia is an elegant endemic plant in Türkiye and it has limited distribution. In this study, cytotoxic effects of ethanolic extracts from different parts and concentrations extracted from I. taochia collected from the surroundings of Tortum (Erzurum), on A549 human lung adenocarcinoma cell line were investigated. Cytotoxicity of extracts were evaluated by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) method. Apopototic activity of IC50 values of extracts were evaluated with Annexin V and Caspase 3/7 assays. Ultrastructural changes of IC50 doses treated cells were investigated by transmission electron microscopy. As a result, it was determined that ethanol extract of I. taochia showed significant cytotoxic activity on A549 cells after 24 hours the extract a dose-dependent reduction in cell viability. IC50 values of above and below ground parts ethanolic extracts were determined as 7 µg/ml and 20 µg/ml respectively. Specifically, apoptosis inducing effect was increased at 7 and 20 µg/ml concentrations by 24 hours. We found that I. taochia ethanol extracts had antiproliferative and apoptotic effects on the human lung adenocarcinoma cells A549. However, further studies at molecular level are required to support our findings and to elucidate chemoproteventive and chemotherapeutic effects of I. taochia on lung cancer.

show abstract

“…Proučavajući antiproliferativnu aktivnost 3-(4-supstituisanih benzil)-5,5-difenilhidantoina i 3-(4-supstituisanih benzil)-5-etil-5-fenilhidantoina, Ušćumlić i saradnici su pokazali značajnu aktivnost ovih molekula prema ćelijskoj liniji humanog karcinoma dojke, MDA--MB-231 i ćelijskoj liniji humanog karcinoma kolona HCT-116. Aktivnost je naročito izražena kada se na fenilnom jezgru kao supstituenti nalaze metil-grupa i halogeni elementi [39][40][41][42].…”

Section: Antiproliferativna Aktivnost Derivata Spirohidantoinaunclassified

Synthesis, structure and biological properties of active spirohydantoin derivatives

Lazić¹,

Valentić²,

Trišović³

et al. 2016

Hem Ind

View full text Add to dashboard Cite

Spirohidantoins represent an pharmacologically important class of heterocycles since many derivatives have been recognized that display interesting activities against a wide range of biological targets. First synthesis of cycloalkanespiro-5-hydantoins was performed by Bucherer and Lieb 1934 by the reaction of cycloalkanone, potassium cyanide and ammonium-carbonate at reflux in a mixture of ethanol and water. QSAR (Quantitative Structure-Activity Relationship) studies showed that a wide range of biological activities of spirohydantoin derivatives strongly depend upon their structure. This paper describes different methods of synthesis of spirohydantoin derivatives, their physico-chemical properties and biological activity. It emphasizes the importance of cycloalkanespiro-5-hydantoins with anticonvulsant, antiproliferative, antipsychotic, antimicrobial and antiinflammatory properties as well as their importance in the treatment of diabetes. Numerous spirohydantoin compounds exhibit physiological activity such as serotonin and fibrinogen antagonist, inhibitors of the glycine binding site of the NMDA receptor also, antagonist of leukocyte cell adhesion, acting as allosteric inhibitors of the protein-protein interactions. Some spirohydantoin derivatives have been identified as antitumor agents. Their activity depends on the substituent presented at position N-3 of the hydantoin ring and increases in order alkene > ester > ether. Besides that, compounds that contain two electron withdrawing groups (e.g. fluorine or chlorine) on the third and fourth position of the phenyl ring are better antitumor agents than compounds with a single electron withdrawing group. [Projekat Ministarstva nauke Republike Srbije, br. 172013]

show abstract

Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116

Cited by 11 publications

References 28 publications

Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation

Fabrication of Sustained Release Curcumin-Loaded Solid Lipid Nanoparticles (Cur-SLNs) as a Potential Drug Delivery System for the Treatment of Lung Cancer: Optimization of Formulation and In Vitro Biological Evaluation

The Impact of Endemic Iris taochia Ethanolic Extracts on Human Lung Adenocarcinoma Cells

Synthesis, structure and biological properties of active spirohydantoin derivatives

Contact Info

Product

Resources

About