Evaluation of Antioxidative Activity for Extracts from Defatted Rice Bran Using 5-Axe Cobweb Chart

Abstract

Defatted rice bran was treated at 230℃ for 5 min with distilled water or 40% (v/v) acetone to produce the corresponding extracts. Acetone-soluble substances were fractionated from the extracts. The antioxidative activities against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl and peroxyl radicals, and the hypochlorite and peroxynitrite ions of the extracts and acetone-soluble substances were examined, and the activities were comprehensively characterized using the 5-axe cobweb chart. The 40% acetone extract exhibited higher activity than the water extract. Fractionation of acetone-soluble substances from extracts increased activity. The acetone-soluble substances from the extract with 40% acetone exhibited the highest activity among the extracts and acetone-soluble substances.

Engineering aspects of rate-related processes in food manufacturing

“…The antioxidative activities of the extracts and fractions were then tested against DPPH, hydroxyl and peroxyl radicals, and hypochlorite and peroxynitrile ions and were characterized using a 5-axe cobweb chart. 122) The mutagenicity of similar extracts prepared at various temperatures was also examined using the Umulac AT test, which showed no activity for both -S9 and + S9. 123) Antioxidative performance for linoleic acid was then further tested for extracts prepared from 120 to 240°C.…”