Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata

Duan, Xiao-Juan; Zhang, Weiwei; Li, Xiaoming; Wang, Bin‐Gui

doi:10.1016/j.foodchem.2004.12.015

Cited by 532 publications

(333 citation statements)

References 21 publications

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“…Another important matter is that ABTS + can be dissolved in aqueous and organic media, in which the antioxidant activity can be measured, due to the hydrophilic and lipophilic nature of the compounds, those existed in the plant cells. In contrast, DPPH has been used extensively as a free radical to evaluate reducing substances (Cotelle et al 1996) and is a useful reagent for investigating the free radical scavenging activities of polyphenol compounds (Duan et al 2006). Many researchers have reported positive correlation between free radical scavenging activity.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

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Ten Darjeeling tea clones (BT15/263, RR17/144, B777, T253, B157, Sundaram, HV39, AV2, K1/1 and TTV1) were collected from the experimental garden of Darjeeling Tea Research and Development Centre, Kurseong. Total phenol, flavonoids and two antioxidating enzymes (peroxidase and superoxide dismutase) were estimated. The total phenol ranged between 241 and 28 GAE mg g −1 of leaf dry weight. The highest amount obtained in four clones, B15/263 (241.47), RR17/144 (221.2), B777 (154.54) and B157 (140.23 mg g −1 ). Flavonoids were estimated as Catechin equivalent (CE) and ranged between 56.88 and 20.81 CE mg g −1 leaf dry weight. Higher amounts occurred in BT15/263 (56.88 mg g −1 ), B777 (56.69) and RR17/144 (48.63). Antioxidant activities were measured following DPPH and ABTS free radicle scavenging procedures and the results were well according to total polyphenol content among the clones (in total phenols, ranges of correlation in DPPH assay were r 2 =0.990-0.989, p≤0.05; in flavonoids r 2 =0.954, p≤0.01-0.987, p≤0.05). Similarly, ABTS percent scavenging results were quiet significant. The IC 50 values were determined for both DPPH and ABTS assay. PAGE expressions of isoforms in two antioxidative enzymes and quantification of them also varied much among the investigated clones. The incidence of total phenols, flavonoids, PRX and SOD and ROS scavenging assay in in-situ condition, might be used as biochemical markers towards the superior adaptability against abiotic stress. In the present work, four clones (B15/263, B777, RR17/144 and B157) would be designated as comparatively better suited to the predicted abiotic stress.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

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show abstract

“…DPPH, superoxide anion radical scavenging activity assays are most commonly accepted ones which have been used in the present investigation [46,47] . DPPH has been used extensively as a free radical to evaluate reducing substances and is a useful reagent for investigating the free radical scavenging activities of compounds [48] . The use of the DPPH radical provides an easy, rapid and convenient method to evaluate the antioxidant and radical scavengers [49,50] .…”

Section: Discussionmentioning

confidence: 99%

Assessment of effect of hydroalcoholic and decoction methods on extraction of antioxidants from selected Indian medicinal plants

Kaneria

Kanani

Chanda

2012

Asian Pacific Journal of Tropical Biomedicine

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“…Scavenging effects on DPPH radicals Scavenging effects of the extracts on DPPH radicals were determined according to Duan et al (2006). Hundred microliters of various concentrations of tea extracts was mixed with 2900 μl DPPH solution (120 μM) in ethanol and incubated at 37˚C for 30 min in the dark.…”

Section: Methodsmentioning

confidence: 99%

Comparative studies on the physicochemical and antioxidant properties of different tea extracts

Chen

2011

J Food Sci Technol

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Tea is one of the most popular drinks next to water. Tea polyphenol is one of the main bioactive constituents of tea with health functions. In order to find the most bioactive tea polyphynols, polyphenol extracts from green tea, black tea and chemical oxidation products of green tea extracts were comparatively studied on the physicochemical and antioxidant properties. Results showed physicochemical and antioxidant properties of polyphenol extracts changed greatly after the chemical oxidation. Hydrogen peroxide induced oxidation products (HOP) possessed the highest antioxidant ability among the four tea polyphenol extracts. Thirteen phenolic compounds and one alkaloid in HOP were identified by reversed phase high-performance liquid chromatography coupled to diode array detection and electrospray ionization mass spectrometry (RP-HPLC-DAD-ESI-MS). Hydrogen peroxide induced oxidation of tea polyphenol extracts could improve the antioxidant activity and could be used to produce antioxidants for food industry.

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Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata

Cited by 532 publications

References 21 publications

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Assessment of effect of hydroalcoholic and decoction methods on extraction of antioxidants from selected Indian medicinal plants

Comparative studies on the physicochemical and antioxidant properties of different tea extracts

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