Evaluation of antioxidant potential of the cultured mycobiont of a lichen Usnea ghattensis

Abstract

The biologically active compounds, antioxidant activities and free radical scavenging effects of dry matter of cultural medium (DMCM), filtrate (DMF) and different solvent extracts of mycobiont from lichen species Usnea ghattensis in submerged culture (UGSC) were investigated. DMF showed the strongest inhibition of lipid peroxidation as a function of its concentration, and was comparable to the antioxidant activity of BHA at the same concentration of 0.2 mg/mL. The hexane extract of mycobiont had the weakest antioxidant ability, whereas other mycobiont extracts exhibited a modest to strong inhibition of lipid peroxidation and in free radical scavenging activity. The antioxidant activities of mycobiont extracts were correlated with the presence of total polyphenol content in the extract. It was found that antioxidant components in methanol, acetone, ethanol and hexane extracts must be derived from the secondary metabolites produced by the lichen mycobiont. The results presented herein indicate that extracts of cultured mycobiont of lichen Usnea ghattensis could possibly act as chemopreventing agent with respect to free radical-related diseases.

Extraction Optimization, Preliminary Characterization and Antioxidant Activity of Glycoproteins from the Muscle of <i>Sepia pharaonis</i>

“…The recent studies indicated that antioxidant activity of glycoproteins was associated with glycosidic linkage, the content of protein and carbohydrate, molecular weight, compositions of amino acid and monosaccharide, etc. (Fuad, 2006;Behera et al, 2005;Liu and Zhao, 2011;Heo and Lim, 2004;Lo et al, 2011;Dahl and Richardson, 1978). Fuad (2006) reported that the O-glycosidic linkage was not important for in vitro bioactivity, but it played an important role in vivo bioactivity.…”

“…However, N-glycosidic linkage was opposite to O-glycosidic linkage. It has been reported that the superoxide radical scavenging activity of polysaccharide extracts appears to depend on the amount of protein or peptide present in the form of polysaccharide-protein or polysaccharide-peptide complexes, lower polysaccharide/protein or polysaccharide/ peptide ratios can exhibit the excellent scavenging ability (Behera et al, 2005). Our results indicated that the increase of protein and carbohydrate contents of glycoproteins through consecutive separation and purification can lead to the improvement of DPPH radical scavenging activity, which is in good agreement with other reports in the literature Liu and Zhao, 2011).…”

Optimization of fermentation media composition of ganoderma lucidum for improved production of antioxidant and antimicrobial compounds

“…[32] Polysaccharopeptide obtained from mushrooms have lower polysaccharide/peptide ratios and exhibits the strongest scavenging effects. [33,34] It was found that EPSP from G. lucidum were complexes of polysaccharides (71.88%) and proteins (28.12%). [35] Perhaps this was why EPSP from G. lucidum had more powerful free radical scavenging capacity and reducing power.…”

Evaluation of Antimicrobial, Antioxidant and Anticancer Activities of Few Macrolichens Collected From Eastern Ghats of Tamil Nadu, India

“…Antioxidant activity of lichens are evidenced by the presence of phenols 17 . A decrease in absorbance of the reaction mixture indicated a higher free radical scavenging activity.…”