Evaluation of Antioxidant Capacity ofSolanum sessiliflorum(Cubiu) Extract: AnIn VitroAssay

Abstract

Cubiu is a vegetable of Solanaceae family, native to the Amazon, which is widely distributed through Brazil, Peru, and Colombia. It is used in food, medicine, and cosmetics by native populations. Research has shown that cubiu extracts have antioxidant activities with great biological relevance. We performed a phytochemical screening to identify the main chemical groups that could confer antioxidant activity to this extract. Several tests and qualitative precipitation specific staining for major classes of secondary metabolites were used. Antioxidant capacity in vitro tests (DPPH and ABTS) were also used to assess the extract's ability to sequester free radicals of 70% hydroethanolic and aqueous extracts of cubiu flour. Alkaloids, organic acids, phenols, flavonoid glycosides, and coumarins were found in the hydroethanolic extract while the aqueous extract presented anthocyanins, gums, tannins and mucilage, amino groups, and volatile and fixed acids. For in vitro tests, the IC50 value obtained in the DPPH assay was 606.3 ± 3.5 μg/mL while that for the ABTS assay was 290.3 ± 10.7 µg/mL. Although cubiu extracts present chemical compounds directly related to antioxidant activity, our results show that it has a low antioxidant activity. Additional studies will be needed to isolate and characterize specific compounds to further assess antioxidant activity.

Hexane extract from Spondias tuberosa (Anacardiaceae) leaves has antioxidant activity and is an anti-Candida agent by causing mitochondrial and lysosomal damages

“…Antioxidant activity of the hexane extract was evaluated by the DPPH scavenging assay according to Mascato et al [ 24 ], with some modifications. The hexane extract was diluted in methanol to reach concentrations ranging from 15.625 to 1000 μg/mL.…”

Five Underutilized Ecuadorian Fruits and Their Bioactive Potential as Functional Foods and in Metabolic Syndrome: A Review

“…The main phenolic in the species is 5-caffeoylquinic acid, which makes its extracts powerful antioxidant scavengers [ 104 ]. There is qualitative indication of alkaloid presence, but no positive identification [ 105 ]. Among the compounds of interest in S. sessiliflorum are caffeic acid, the derivatives of which have been patented as hypoglucemiants [ 106 ].…”

Antihyperlipidemic and Antioxidant Capacities, Nutritional Analysis and UHPLC-PDA-MS Characterization of Cocona Fruits (Solanum sessiliflorum Dunal) from the Peruvian Amazon

“…These adaptive morphotypes of the fruit depends directly on various biological factors and has been diversifying during the evolution and natural selection and can have the ability to produce different biologically important metabolites. Several carotenoids, alkaloids, organic acids, phenolic acids, flavonoid glycosides, coumarins, tannins, and volatile and fixed acids were reported to occur in fresh cocona fruits from Brazil [1,2] and recently, caffeoyl quinic acid was reported as the main important phenolic in the fruits [3], whereas determination of volatile organic compounds (VOCs) was done by HS-SPME/GC-MS in some ecotypes from Brazil [4], besides, extracts of those Brazilian ecotypes presented high concentrations of caffeic and gallic acids, beta-carotene, catechin, quercetin, and rutin and showed low density lipoproteins oxidation; cytotoxic and antiproliferative effect on breast (MCF-7) and colorectal (HT-29) cancer cell lines [5]. The purpose of this work is to contribute to the full phytochemical study of the Peruvian ecotype species through UHPLC-PDA-ESI-OT-MS for full untargeted metabolomic analyses to promote in fruit growers and fruit processors in the Peruvian Amazon the adoption of strategies for the sustainable use of the more promising ones based on its phenolic content and intrinsic health related properties, such as antihyperlipidemic capacities of the studied five ecotypes of this highly consumed fruit from Peru.…”