Abstract:The study presents original results concerning analytical characterization of alcoholic extracts of four wild berries (blueberries, blackberries, red currants and raspberries) and two cultivated berries (black cherries and strawberries). Total and some individual phenols concentrations were determined using molecular absorption spectrometry (modified Folin Ciocalteu method) and HPLC-DAD technique. The measurement of lipid-soluble antioxidant capacity (ACL) was done by photochemiluminescence method using trolox… Show more
“…Identification and quantitative determination of the active constituents from samples macerates was done by comparing the chromatogram of standards mixture. The total phenols were estimated according to the Folin-Ciocalteau method [22][23][24][25][26][27][28].…”
Section: Identification and Quantification Of Phenolic Compounds By Hmentioning
confidence: 99%
“…The antioxidant capacity was evaluated using DPPH Radical Scavenging test. Gallic acid (GA) was used as standard to plot calibration curves and the results were expressed as equivalents (mg GAE) [14,16,[27][28][29][30][31][32]. In 25 mL calibrated flasks different volume of gallic acid solutions were added, then 5 mL DPPH 0.063% (1.268 mM) in methanol, filled up to the mark with methanol and let in the dark, to the room temperature for 45 minutes before the absorbance registration at 530 nm versus methanol.…”
The total phenols concentration in two sage macerates has been estimated by Folin-Ciocâlteau method, identified and quantified using HPLC-DAD method in order to assess the biological activity. The results for total phenols values of Folin Ciocalteau method indicate that Salvia officinalis L macerate S2, presents a higher amount of phenolic compounds than macerate S1. By HPLC-DAD method, six individual phenolic compounds were identified in sage macerates among which where cinnamic acid was found in highest concentration (652.478 mg/100g d.w. in S2 and 473.381 mg/100g d.w. in S1). The antioxidant activity of sage macerates was evaluated using DPPH Radical Scavenging test. Sage macerates exhibited high antioxidant activity, between 439.5 mg GAE /mL and 400 mg GAE /mL. Antibacterial activity of sage macerates was evaluated against 20 Gram positive and Gram negative bacterial strains isolated from clinical specimens. Both macerates showed significant but variable antibacterial activity with inhibition zones ranging from 4.97 mm (S2) to 7.28.mm (S1). The effect was stronger on Gram positive (Enterococcus, Staphyococcus) than Gram negative bacteria (Escherichia sp, Proteus sp, Klebsiella sp). Eleven metals concentrations were determined by AAS method in sage leaves; it has been found that Cd, Ni and Pb concentrations are below the detection limits.
“…Identification and quantitative determination of the active constituents from samples macerates was done by comparing the chromatogram of standards mixture. The total phenols were estimated according to the Folin-Ciocalteau method [22][23][24][25][26][27][28].…”
Section: Identification and Quantification Of Phenolic Compounds By Hmentioning
confidence: 99%
“…The antioxidant capacity was evaluated using DPPH Radical Scavenging test. Gallic acid (GA) was used as standard to plot calibration curves and the results were expressed as equivalents (mg GAE) [14,16,[27][28][29][30][31][32]. In 25 mL calibrated flasks different volume of gallic acid solutions were added, then 5 mL DPPH 0.063% (1.268 mM) in methanol, filled up to the mark with methanol and let in the dark, to the room temperature for 45 minutes before the absorbance registration at 530 nm versus methanol.…”
The total phenols concentration in two sage macerates has been estimated by Folin-Ciocâlteau method, identified and quantified using HPLC-DAD method in order to assess the biological activity. The results for total phenols values of Folin Ciocalteau method indicate that Salvia officinalis L macerate S2, presents a higher amount of phenolic compounds than macerate S1. By HPLC-DAD method, six individual phenolic compounds were identified in sage macerates among which where cinnamic acid was found in highest concentration (652.478 mg/100g d.w. in S2 and 473.381 mg/100g d.w. in S1). The antioxidant activity of sage macerates was evaluated using DPPH Radical Scavenging test. Sage macerates exhibited high antioxidant activity, between 439.5 mg GAE /mL and 400 mg GAE /mL. Antibacterial activity of sage macerates was evaluated against 20 Gram positive and Gram negative bacterial strains isolated from clinical specimens. Both macerates showed significant but variable antibacterial activity with inhibition zones ranging from 4.97 mm (S2) to 7.28.mm (S1). The effect was stronger on Gram positive (Enterococcus, Staphyococcus) than Gram negative bacteria (Escherichia sp, Proteus sp, Klebsiella sp). Eleven metals concentrations were determined by AAS method in sage leaves; it has been found that Cd, Ni and Pb concentrations are below the detection limits.
“…The Black Sea is a great resource due to its geographical position in relation to the planetary Ocean, the character of semi-closed, a huge catchment area, as well as its unique hydro-biological features make the Black Sea a highly sensitive ecosystem, exposed to pollution [1]. Changes in the parameters of the physical, chemical and biological agents of the Black Sea is due to the anthropic impact on the entire basin, and eutrophication is hastened by the vast quantities of biogenic sources offered by the Danube, the Dnieper and the Dniester [2,3]. With increasing human population, pollution has become a great concern.…”
Knowledge of environmental pollution with an impact on health is essential for a sustainable environment and useful for people. The coastal areas of the world�s seas and oceans are polluted with different pollutants from technological sources and from other sources of socio-human activities. The pollutants studied are heavy metals Cd, Cu, Zn and Pb. In the paper are presented researches regarding the heavy metal concentrations determined in samples from marine water, sediments and algal mass, harvested from six stations from different areas of the Romanian Black Sea in two different years 2017 and 2018. The analyses were performed in the Chemistry Laboratories of the Faculty of Pharmacy, Ovidius University in Constanta, by a spectrophotometric method using Atomic Absorption Spectrometer ContrAA-700, Analytic Jena AG. For marine water in 2018 the following concentrations were obtained: Zn ] Pb ] Cu ] Cd and in 2017 the order was different: Zn ] Cu ] Pb ] Cd. For sediments in 2017 the order of concentrations is Cu ] Zn ] Pb ] Cd and in 2018 the highest values in the sediment are recorded by the next high values Cu ] Pb ] Zn ] Cd. Constant climate change and increased eutrophication in recent years have led to a massive increase in marine biomass in the Black Sea. For seaweed samples there are accumulations in the order of Zn ] Cu ] Pb ] Cd in both years. Bioaccumulation factors in water BCF water, were higher in algae for Zn and Cu. From the analysis of bioaccumulation factors, it can be noticed that seawater algae accumulate heavy metals from seawater and sediment (confirmed by BCF water ] 1 or BCF sed. ] 1). The accumulation of heavy metals in seaweed shows the existence of pollutants and marine pollution factors. Marine algae are the best biomarkers of pollution.
“…Okan et al (2018) have found that the content of phenolic compounds and flavonoids in 28 varieties of blueberries from Turkey varied in the range 76.20-215.12 mg GAE/100g FW and 30.44-91.69 mg quercetin/100g FW, respectively. Stanciu et al (2019) analyzed some berries (blueberry, raspberry, blackberry and red currant) and showed that the highest total phenolic content was registered for blueberries (543.50 mg GAE/100g FW), followed by red currants (432.30 mg GAE/100gFW), blackberries (425.00 mg GAE/100gFW), and raspberries (344.50 mg GAE/100gFW).…”
Section: Total Phenolics Flavonoids and Anthocyaninsmentioning
The objective of this paper was to evaluate seven different berries most consumed in Romania in order to determine the bioactive compounds: total phenolics content, total flavonoid content, total anthocyanins content, antioxidant activity by DPPH and total antioxidant capacity (water-soluble and lipid-soluble by photochemiluminescence assay, PCL). The analyzed fruits contain significant amounts of phenolic compounds between 18.91 and 383.06 mg gallic acid equivalents per 100 grams of fresh berries. Black currant extract had the highest content of total phenolic compounds, 383.06 mg GAE/100g, while the lowest level was obtained for the gooseberry, 18.91 mg GAE/100g. DPPH values varied between 22.15 and 892.35μmol Trolox Equivalent/100g fresh berries. Strong positive correlations between the antioxidant capacity of lipid-soluble compounds measured by PCL and DPPH was found (r= 0.9153). The results obtained demonstrated that different Romanian berries studied show a great antioxidant activity with possible benefits on human health.
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