Evaluation of antioxidant and inhibitory properties of Citrus aurantium L. on the acetylcholinesterase activity and the production of amyloid nano–bio fibrils

Pasandideh, Shayan; Arasteh, Amir

doi:10.1016/j.ijbiomac.2021.04.043

Cited by 15 publications

(11 citation statements)

References 30 publications

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“…Using GC-MS analysis, the same authors identified seventy-seven compounds in the essential oil of C. aurantium flowers, with the most frequent chemical classes being oxygenated monoterpenes, aliphatic hydrocarbons, monoterpene hydrocarbons, and esters [16]. Similarly, the most frequent detected compounds in C. aurantium petals powder were: D-glucuronic acid, D-limonene, octadecenoic acid, daphnetin, hexadecanoic acid, linalool, pyrrolidinone, and phthalic acid [18]. The latter compounds were listed in descending order: 10%, 5.5%, 4%, 3.7%, 2%, 2%, 1.2%, and 1% respectively.…”

Section: Aurantium Flowersmentioning

confidence: 97%

“…Sodium cumene sulphonate (Na-CuS) and sodium salicylate (Na-Sal) were used in this study. Ethanol, methanol, and hot water were used to extract phenolic compounds from all C. aurantium parts [11,13,17,18,21,22,24,29]. Active constituents from C. aurantium fruits and juice were also extracted using 80% acetone solvent [77] and ether-pentane (1:1) [12] respectively.…”

Section: Solvent Extractionmentioning

confidence: 99%

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Citrus aurantium L. Active Constituents, Biological Effects and Extraction Methods. An Updated Review

et al. 2021

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Citrus genus is a prominent staple crop globally. Long-term breeding and much hybridization engendered a myriad of species, each characterized by a specific metabolism generating different secondary metabolites. Citrus aurantium L., commonly recognized as sour or bitter orange, can exceptionally be distinguished from other Citrus species by unique characteristics. It is a fruit with distinctive flavor, rich in nutrients and phytochemicals which possess different health benefits. This paper presents an overview of the most recent studies done on the matter. It intends to provide an in-depth understanding of the biological activities and medicinal uses of active constituents existing in C. aurantium. Every plant part is first discussed separately with regards to its content in active constituents. All extraction methods, their concepts and yields, used to recover these valuable molecules from their original plant matrix are thoroughly reported.

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Section: Aurantium Flowersmentioning

confidence: 97%

Section: Solvent Extractionmentioning

confidence: 99%

Citrus aurantium L. Active Constituents, Biological Effects and Extraction Methods. An Updated Review

et al. 2021

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“…The extract obtained from blossoms of Citrus aurantium containing essential oils exhibited anti-acetylcholinesterase, inhibitory properties on the production of amyloid nanobiofibrils and antioxidant activity. The extract showed acetylcholinesterase inhibition with IC 50 = 42.8 mg/mL [ 19 ]. The inhibitory effects of fragrant essential oils obtained from Citrus aurantium on the production of amyloid nanobiofibrils from bovine serum albumin was also investigated [ 19 ].…”

Section: Determination Of Anti-neurodegenerative Disease Activity Of ...mentioning

confidence: 99%

“…Free 2-nitro-5-mercaptobenzoic acid has a yellow color. Currently, measurements are most often carried out with the application of 96-well microplate [ 17 , 18 ], rarely using a spectrophotometer [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Neurodegenerative diseases are also characterized by the extraneuronal accumulation of β-amyloid peptide. The accumulation of aggregated proteins at neurons has been correlated with Alzheimer’s disease patients, who show the presence of amyloid fibrils in the brain, which indicates the relationship between amyloid fibrils and the disease [ 19 ]. The accumulation of this toxic peptide leads to the deposition of β-amyloid into plaques and is thought to drive a pathologic cascade, which culminates in neuronal death [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Determination of Anti-Alzheimer’s Disease Activity of Selected Plant Ingredients

Tuzimski

Petruczynik

2022

Molecules

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Neurodegenerative diseases, among which one of the more common is Alzheimer’s disease, are the one of the biggest global public health challenges facing our generation because of the increasing elderly population in most countries. With the growing burden of these diseases, it is essential to discover and develop new treatment options capable of preventing and treating them. Neurodegenerative diseases, among which one of the most common is Alzheimer’s disease, are a multifactorial disease and therefore demand multiple therapeutic approaches. One of the most important therapeutic strategies is controlling the level of acetylcholine—a neurotransmitter in cholinergic synapses—by blocking the degradation of acetylcholine using acetylcholinesterase inhibitors such as tacrine, galantamine, donepezil and rivastigmine. However, these drugs can cause some adverse side effects, such as hepatotoxicity and gastrointestinal disorder. Thus, the search for new, more effective drugs is very important. In the last few years, different active constituents from plants have been tested as potential drugs in neurodegenerative disease therapy. The availability, lower price and less toxic effects of herbal medicines compared with synthetic agents make them a simple and excellent choice in the treatment of neurodegenerative diseases. The empirical approach to discovering new drugs from the systematic screening of plant extracts or plant-derived compounds is still an important strategy when it comes to finding new biologically active substances. The aim of this review is to identify new, safe and effective compounds that are potential candidates for further in vivo and clinical tests from which more effective drugs for the treatment of Alzheimer’s disease could be selected. We reviewed the methods used to determine anti-Alzheimer’s disease activity. Here, we have discussed the relevance of plant-derived compounds with in vitro activity. Various plants and phytochemical compounds have shown different activity that could be beneficial in the treatment of Alzheimer’s disorders. Most often, medicinal plants and their active components have been investigated as acetylcholinesterase and/or butyrylcholinesterase activity inhibitors, modifiers of β-amyloid processing and antioxidant agents. This study also aims to highlight species with assessed efficacy, usable plant parts and the most active plant components in order to identify species and compounds of interest for further study. Future research directions are suggested and recommendations made to expand the use of medicinal plants, their formulations and plant-derived active compounds to prevent, mitigate and treat Alzheimer’s disease.

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Design and investigation of celastrol‐peptide nanoassemblies and their binding interactions with superoxide dismutase 1 and its mutants

Goncalves,

Phan,

Biggs

et al. 2024

Nano Select

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The misfolding and aggregation of superoxide dismutase 1 (SOD1) and its mutants has been implicated in amyotrophic lateral sclerosis (ALS). In this study, we have created three peptide conjugates with the antioxidant pentacyclic terpene celastrol and examined their interactions with SOD1 and its mutants A4V and G93A. The peptides YYIVS, MPDAHL, and GSGGL are derived from natural sources and are known for their inherent antioxidant properties. Docking studies revealed that most conjugates showed strong binding with the metal binding and electrostatic loops as well as the β1, β5, and β6 hydrophobic core of SOD1. The conjugates were synthesized and self‐assembled into nanoassemblies. Surface plasmon resonance studies further confirmed the binding interactions of the nanoassemblies with the SOD1 proteins. The nanoassemblies were found to internalize into HEK293T cells. The HEK 293T cells were then transfected with GFP fused WT (Wild Type), A4V and G93A SOD1 mutants. Flow cytometry revealed that treatment with celastrol‐peptide nanoassemblies, affected the fluorescence of the SOD1 protein, implying their role in modulating SOD1, particularly for the mutants. N–Acetyl–Leu–Leu–Norleucinal (ALLN) induced SOD1 aggregation was also affected upon treatment with the nanoassemblies. These results suggest that the nanoassemblies may potentially modulate the activity and structure of SOD1.

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Evaluation of antioxidant and inhibitory properties of Citrus aurantium L. on the acetylcholinesterase activity and the production of amyloid nano–bio fibrils

Cited by 15 publications

References 30 publications

Citrus aurantium L. Active Constituents, Biological Effects and Extraction Methods. An Updated Review

Citrus aurantium L. Active Constituents, Biological Effects and Extraction Methods. An Updated Review

Determination of Anti-Alzheimer’s Disease Activity of Selected Plant Ingredients

Design and investigation of celastrol‐peptide nanoassemblies and their binding interactions with superoxide dismutase 1 and its mutants

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