Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts

Emsen, Bugrahan; Türkez, Hasan; Toğar, Başak; Aslan, Ali

doi:10.1177/0960327116650012

Cited by 18 publications

(10 citation statements)

References 29 publications

(42 reference statements)

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“…Especially after the 1990s, new usage areas of medicinal and aromatic plants increase the use of these plants day by day [Lange 2006]. Naturally occurring substances in many plant species are used in various treatments and show positive results [Emsen et al 2016[Emsen et al , 2017. This study presents antioxidant activities of water and methanol extracts of C. demersum propagated via tissue culture techniques.…”

Section: Resultsmentioning

confidence: 97%

EVALUATION OF ANTIOXIDANT ACTIVITY OF in vitro PROPAGATED MEDICINAL Ceratophyllum demersum L. EXTRACTS

Emsen

Doğan

2018

Acta Sci.Pol.Hortorum Cultus

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Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25-1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC 50 value for chelating on ferrous ions was 9.24 mg/mL, EC 50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.

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Section: Resultsmentioning

confidence: 97%

EVALUATION OF ANTIOXIDANT ACTIVITY OF in vitro PROPAGATED MEDICINAL Ceratophyllum demersum L. EXTRACTS

Emsen

Doğan

2018

Acta Sci.Pol.Hortorum Cultus

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“…While Thadhani et al (2011) used sekikaic and lecanoric acid in these treatments, Grujičić et al (2014) revealed antioxidant capacity of methanol extract obtained from Cetraria islandica. Another lichen-antioxidant related study was carried out by testing physodic and olivetoric acid obtained from Pseudevernia furfuracea on human amnion fibroblasts and it was detected that these metabolites increased the antioxidant capacity of the cells (Emsen et al 2017). Otherwise, studies on the oxidant properties of lichens are also common (Paudel et al 2012;Ghate et al 2013;Kumar et al 2014).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Investigation of the Anticancer Activity of Friedelin in Glioblastoma Multiforme

Emsen¹,

Engin²,

Türkez³

2018

AKU-J. Sci. Eng.

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There are different herbal methods used for support in many cancer diseases. Lichens are important organisms containing unique herbal compounds and it is known that they have different anticancer activities. Starting from these features, the present study was aimed to investigate anticancer activity of friedelin (FRI), a lichen compound against glioblastoma multiforme (GBM) showed dangerous malignant properties within brain cancer species. It was used human U87MG-GBM cancer cell lines and primary rat cerebral cortex (PRCC) non-cancerous cells isolated from Sprague-Dawley rats in order to determine side effect level of FRI. In the experiments, cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) tests), antioxidant (via total antioxidant capacity (TAC) test), pro-oxidant (via total oxidative stress (TOS) test) and genotoxic (via 8hydroxy-2′-deoxyguanosine (8-OH-dG) test) activities of different concentrations of FRI were tested. As a result of the study, MTT assay revealed that FRI showed higher cytotoxic activity on U87MG cells compared to PRCC cells (median inhibitory concentration (IC50): 46.38 and 1271.77 mg/L, respectively). Based on U87MG cells, it was determined a significant positive correlation between LDH and TOS activities. High positive correlation between TAC and cell viability on healthy PRCC cells exhibited antioxidant capacity of FRI. Consequently, the results obtained from the present study proved the potential of natural product with an antioxidant capacity as a source for anticancer compound against GBM.

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“…Physodic acid was also investigated as an antioxidant agent, showing high DPPH radical scavenging activity (IC 50 value of 69.11 μg/mL), high SOR scavenging activity (IC 50 value of 118.17 μg/mL), and high reducing power [101]. Lower concentrations of physodic acid tested in cultured human amnion fibroblasts (< 50 mg/L) and cultured human lymphocytes (0.5-10 mg/ L) showed antioxidant capacities [108,109].…”

Section: Physodic Acidmentioning

confidence: 99%

Lichen Depsidones with Biological Interest

et al. 2021

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Depsidones are some of the most abundant secondary metabolites produced by lichens. These compounds have aroused great pharmacological interest due to their activities as antioxidants, antimicrobial, and cytotoxic agents. Hence, this paper aims to provide up-to-date knowledge including an overview of the potential biological interest of lichen depsidones. So far, the most studied depsidones are fumarprotocetraric acid, lobaric acid, norstictic acid, physodic acid, salazinic acid, and stictic acid. Their pharmacological activities have been mainly investigated in in vitro studies and, to a lesser extent, in in vivo studies. No clinical trials have been performed yet. Depsidones are promising cytotoxic agents that act against different cell lines of animal and human origin. Moreover, these compounds have shown antimicrobial activity against both Gram-positive and Gram-negative bacteria and fungi, mainly Candida spp. Furthermore, depsidones have antioxidant properties as revealed in oxidative stress in vitro and in vivo models. Future research should be focused on further investigating the mechanism of action of depsidones and in evaluating new potential actions as well as other depsidones that have not been studied yet from a pharmacological perspective. Likewise, more in vivo studies are prerequisite, and clinical trials for the most promising depsidones are encouraged.

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Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts

Cited by 18 publications

References 29 publications

EVALUATION OF ANTIOXIDANT ACTIVITY OF in vitro PROPAGATED MEDICINAL Ceratophyllum demersum L. EXTRACTS

EVALUATION OF ANTIOXIDANT ACTIVITY OF in vitro PROPAGATED MEDICINAL Ceratophyllum demersum L. EXTRACTS

In Vitro Investigation of the Anticancer Activity of Friedelin in Glioblastoma Multiforme

Lichen Depsidones with Biological Interest

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