Evaluation of Antioxidant Activity and Polyphenols Content of the Hydro-methanolic Extract from Saccocalyx satureioides Coss and Dur

Abstract

This study aimed to estimate the total phenolic and flavonoid contents and to evaluate the antioxidant activity of the Hydro-methanolic from Saccocalyx satureioides Coss and Dur aerial part. Total polyphenols contents were determined using Folin-Ciocalteu’s reagent. The flavonoids were estimated using the method of Aluminum chloride (AlCl3). The antioxidant capacity was evaluated using two in vitro models (the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power). Total phenolic and flavonoid content in the extract were 171.34 ±1.43 mg Gallic acid equivalent/g of dry extract (GAE/g) and 18.6 ± 0.46 mg Quercetin equivalent / g of dry extract (QE/g), respectively. The methanol extract has an important capacity to scavenge the free radical DPPH with an IC50 of 0.03 ± 0. 0024 mg/ml. In addition, the plant extract exhibited high reducing power with an IC50 of 0.54 ± 0.00625 mg/ml. The results of the present study may prove that the medicinal plants are a good resource of natural antioxidants.
 Keywords: Antioxidant activity, Saccocalyx satureioides, polyphenols, flavonoids.

Evaluation of Antioxidant activities and total phenolic content of hydro-ethanol extract from Phlomis bovei De Noé areal parts

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