Evaluation of antioxidant activities of principal carotenoids available in water spinach (Ipomoea aquatica)

Fu, Hongfei; Xie, Bijun; Ma, ShaoJun; Zhu, Xuemei; Fan, Gang; Pan, Siyi

doi:10.1016/j.jfca.2010.08.007

Cited by 59 publications

(46 citation statements)

References 29 publications

(30 reference statements)

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“…Recently, violaxanthin was reported to have strong antioxidant properties in oxidative stress and function as a chain breaking antioxidant in lipid peroxidation. 20) Therefore, the antioxidant ability of violaxanthin may be originated from inhibition of iNOS and COX-2 expression.…”

Section: Discussionmentioning

confidence: 99%

“…19) Violaxanthin from water spinach (Ipomoea aquatica) was reported to have a more potent scavenging ability than lutein and β-carotene in ABTS radical-scavenging, inhibition of red blood cell hemolysis, and inhibition of lipid peroxidation in liver. 20) However, the potential anti-inflammatory effects of major carotenoids from C. ellipsoidea have not yet been investigated.…”

mentioning

confidence: 99%

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Anti-inflammatory Effects of Violaxanthin Isolated from Microalga <i>Chlorella ellipsoidea</i> in RAW 264.7 Macrophages

Soontornchaiboon

Joo

Kim

2012

Biological & Pharmaceutical Bulletin

124

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Violaxanthin is a major carotenoid of microalgae Chlorella ellipsoidea and is also found in dark-green leafy vegetables, such as spinach. In this study, the anti-inflammatory effect of violaxanthin isolated from C. ellipsoidea was examined using lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophage cells. In addition, the anti-inflammatory activity and mechanism of action of purified violaxanthin was assessed using various assays, such as quantitative real-time polymerase chain reaction (PCR), Western blotting, and electrophoretic-mobility shift assay (EMSA). The results of this combined analysis revealed that violaxanthin significantly inhibited nitric oxide (NO) and the prostaglandin E 2 (PGE 2 ). Interestingly, violaxanthin effectively inhibited LPS-mediated nuclear factor-κB (NF-κB) p65 subunit translocation into the nucleus, suggesting that the violaxanthin anti-inflammatory activity may be based on inhibition of the NF-κB pathways. In conclusion, violaxanthin of C. ellipsoidea holds promise for use as a potential anti-inflammatory agent for either therapeutic or functional adjuvant purposes.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Anti-inflammatory Effects of Violaxanthin Isolated from Microalga <i>Chlorella ellipsoidea</i> in RAW 264.7 Macrophages

Soontornchaiboon

Joo

Kim

2012

Biological & Pharmaceutical Bulletin

124

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“…It grows wild and is cultivated throughout Africa, Asia, and Southwest Pacific Islands such as Vietnam, China, India, Hong Kong, and Taiwan (1)(2)(3). It is a rich source of phytochemical compounds, particularly carotenoids and chlorophylls (3)(4)(5). Recent studies have reported that water spinach has inhibitory effects against prostaglandin synthesis, liver diseases, eye diseases, and constipation, and has an oral hypoglycemic effect in healthy subjects (1,5).…”

Section: Introductionmentioning

confidence: 99%

Effects of different cooking methods on bioactive compound content and antioxidant activity of water spinach (Ipomoea aquatica)

Thi

Hwang

2015

Food Sci Biotechnol

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Water spinach (Ipomoea aquatica) is a green leafy vegetable commonly found in Vietnam and other tropical countries. We identified the best extraction solvent and compared the effects of different cooking methods on phytochemical contents and antioxidant activity of water spinach. Steamed water spinach extracted with 80% ethanol had the highest amounts of 35.0 mg/g total chlorophylls, 7.1 mg/g carotenoids, 35.6 mg/g total polyphenols, and 40.4 mg/g total flavonoids. The steaming process showed higher antioxidant activity than the uncooking or boiling process. The 80% ethanol extract showed a higher level of antioxidant activities than the water extract. The antioxidant activity was measured with the DPPH, ABTS, hydroxyl and superoxide anion racial scavenging assays as well as the reducing power activity assay were increased in a concentration dependent manner. The overall results support that water spinach contains bioactive compounds and they may play a positive role in our health.

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“…Many carotenoids are found to be strong antioxidants because of their unsaturated groups, and much research has been done in the area of antioxidative assays of carotenoids (Fu et al, 2011). Tomato waste extracts which contained considerable amounts of carotenoids (lycopene and β-carotene) exhibited good antioxidant, and the carotenoid contents exhibited a strong correlation with antioxidant (Stajcˇic´ et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Identification and antioxidant activity of carotenoids from superfine powder of Rhodobacter sphaeroides

Kong

Bo-di

et al. 2017

Emir J Food Agric

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The interest in carotenoids from the natural-antioxidant point of view has recently risen sharply because of their substantial health benefits. Here, we report the identification and antioxidant activity of carotenoids extracted by superfine grinding from Rhodobacter sphaeroides 3757 which is a species of photosynthetic bacteria. After separation and purification by silica gel column chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), the four major carotenoids from the superfine powder of R. sphaeroide 3757 were identified as bixin, hydroxyspheroidenone, 3,3¢,4¢-tetrahydrospirilloxanthin-20-al, and spheroidenone by reversed phase - high performance liquid chromatography - atmospheric pressure chemical ionization - mass spectrometry (RP-HPLC-APCI-MS). The antioxidant activity of the carotenoids extracted after superfine grinding of dry biomass of R. sphaeroide 3757 was higher than that after ultrasonic treatment. When the ratio solvent-to-solid was 30, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, reducing power, and lipid peroxidation inhibitory activity of the extracts after superfine grinding were 74.0% ± 3.1%, 0.497 ± 0.022, and 77.6% ± 3.2%, respectively. By contrast, the extracts after ultrasonic treatment, these numbers were 61.0% ± 2.5%, 0.328 ± 0.014, and 55.2% ± 2.3%, respectively. These results indicate that carotenoids from R. sphaeroide 3757 show a significant antioxidant activity in vitro in a concentration-dependent manner, and that superfine grinding is the optimal extraction method. We hope to provide scientific guidance for commercial production of natural- antioxidant and functional food from carotenoids of R. sphaeroide.

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Evaluation of antioxidant activities of principal carotenoids available in water spinach (Ipomoea aquatica)

Cited by 59 publications

References 29 publications

Anti-inflammatory Effects of Violaxanthin Isolated from Microalga <i>Chlorella ellipsoidea</i> in RAW 264.7 Macrophages

Anti-inflammatory Effects of Violaxanthin Isolated from Microalga <i>Chlorella ellipsoidea</i> in RAW 264.7 Macrophages

Effects of different cooking methods on bioactive compound content and antioxidant activity of water spinach (Ipomoea aquatica)

Identification and antioxidant activity of carotenoids from superfine powder of Rhodobacter sphaeroides

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