Evaluation of antimicrobial effects of novel implant materials by testing the prevention of biofilm formation using a simple small scale medium-throughput growth inhibition assay

Patenge, Nadja; Arndt, K.; Eggert, Thorsten; Zietz, Carmen; Kreikemeyer, Bernd; Bader, Rainer; Nebe, Barbara; Straňák, Vítězslav; Hippler, R.; Podbielski, Andreas

doi:10.1080/08927014.2012.671305

Cited by 23 publications

(15 citation statements)

References 40 publications

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“…In vitro tests with silver-containing coatings on titanium surfaces have been shown to be effective against S. aureus and S. epidermidis [28,29]. Some authors tested other metal ions concerning their biocompatibility and antimicrobial activity in such settings and found that copper showed a high antibacterial effectiveness in relation to its cytotoxicity [11,14,30,31].…”

Section: Discussionmentioning

confidence: 98%

“…The ionized forms of silver, copper, and other metal ions are known for antibacterial and cytotoxic effects [10,11] depending on their concentration and chemical bonding. Although the molecular mechanisms of action are not understood, fully these effects have resulted in a broad spectrum of clinical applications [12,[14][15][16]. For example, with respect to wound management, silver-impregnated dressings are used for care of burns and chronically infected wounds [17,18].…”

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confidence: 99%

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Influence of Antimicrobial Coatings of Vacuum-Assisted Closure Dressings on Methicillin-ResistantStaphylococcus aureusGrowth Kinetics: An In Vitro Study

Ellenrieder

Redanz

Bader

et al. 2015

Surgical Infections

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Influence of Antimicrobial Coatings of Vacuum-Assisted Closure Dressings on Methicillin-ResistantStaphylococcus aureusGrowth Kinetics: An In Vitro Study

Ellenrieder

Redanz

Bader

et al. 2015

Surgical Infections

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show abstract

“…Estimation of the antibacterial potential against S. epidermidis on test samples was completed according to the protocols described earlier[37,38]. The biofilm-forming strain of S. epidermidis (ATCC 35984, American Type Culture Collection, Manassas, VA, United States) was routinely cultured on Columbia blood agar plates (Thermo Fisher Scientific, Waltham, MA, United States).…”

Section: Methodsmentioning

confidence: 99%

Copper as an alternative antimicrobial coating for implants - an in vitro study

Bergemann

Zaatreh

Wegner

et al. 2017

WJT

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AIMTo investigate osteoconductive and antimicrobial properties of a titanium-copper-nitride (TiCuN) film and an additional BONIT® coating on titanium substrates.METHODSFor micro-structuring, the surface of titanium test samples was modified by titanium plasma spray (TPS). On the TPS-coated samples, the TiCuN layer was deposited by physical vapor deposition. The BONIT® layer was coated electrochemically. The concentration of copper ions released from TiCuN films was measured by atomic absorption spectrometry. MG-63 osteoblasts on TiCuN and BONIT® were analyzed for cell adhesion, viability and spreading. In parallel, Staphylococcus epidermidis (S. epidermidis) were cultivated on the samples and planktonic and biofilm-bound bacteria were quantified by counting of the colony-forming units.RESULTSField emission scanning electron microscopy (FESEM) revealed rough surfaces for TPS and TiCuN and a special crystalline surface structure on TiCuN + BONIT®. TiCuN released high amounts of copper quickly within 24 h. These release dynamics were accompanied by complete growth inhibition of bacteria and after 2 d, no planktonic or adherent S. epidermidis were found on these samples. On the other hand viability of MG-63 cells was impaired during direct cultivation on the samples within 24 h. However, high cell colonization could be found after a 24 h pre-incubation step in cell culture medium simulating the in vivo dynamics closer. On pre-incubated TiCuN, the osteoblasts span the ridges and demonstrate a flattened, well-spread phenotype. The additional BONIT®coating reduced the copper release of the TiCuN layer significantly and showed a positive effect on the initial cell adhesion.CONCLUSIONThe TiCuNcoating inhibits the formation of bacterial biofilms on orthopedic implants by influencing the “race for the surface” to the advantage of osteoblasts.

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“…A number of implant surface modifications have been developed to inhibit bacterial colonization by release of bactericidal agents. For example, it was found that sufficient and long lasting copper release is needed to inhibit and neutralize all bacteria (more resistant biofilm) [24][25][26].…”

Section: Introductionmentioning

confidence: 98%

Influence of Cu–Ti thin film surface properties on antimicrobial activity and viability of living cells

Wojcieszak

Kaczmarek

Antosiak

et al. 2015

Materials Science and Engineering: C

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Evaluation of antimicrobial effects of novel implant materials by testing the prevention of biofilm formation using a simple small scale medium-throughput growth inhibition assay

Cited by 23 publications

References 40 publications

Influence of Antimicrobial Coatings of Vacuum-Assisted Closure Dressings on Methicillin-ResistantStaphylococcus aureusGrowth Kinetics: An In Vitro Study

Influence of Antimicrobial Coatings of Vacuum-Assisted Closure Dressings on Methicillin-ResistantStaphylococcus aureusGrowth Kinetics: An In Vitro Study

Copper as an alternative antimicrobial coating for implants - an in vitro study

Influence of Cu–Ti thin film surface properties on antimicrobial activity and viability of living cells

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