Evaluation of Antimicrobial, Antioxidant and Cytotoxic Activities of Dialium cochinchinensis Seed Extract

Bui, L. T. K.; Nguyen, Quoc Tuan; Dao, Linrui; Nguyen, Hoang Duy; Lam, Matthew; Hoang, Cao Thi Kim

doi:10.36468/pharmaceutical-sciences.594

Cited by 8 publications

(5 citation statements)

References 16 publications

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“…According to present study, the concentration of extract and percentage inhibition showed a linear relationship which means that the higher the concentration of plant extract the more is the scavenging property which is similar to the Bui et al [58] .…”

Section: Resultssupporting

confidence: 82%

In Vitro Antioxidant Assay of Different Phytoconstituents and Extraction Optimization of Phenolics from Cyperus rotundus L

Varshney¹,

Prakash²,

Singh³

et al. 2023

IJPS

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Oxidative stress related disorders occur as a result of free radicals in the cellular organs. Antioxidants provide protection contrary to oxidative stress by counteracting free radicals through the help of different bioactive constituents present in the plants. The aim of this study is to find out the quantitative estimation of phytochemicals (phenolics, tannins, flavonoid, non-tannins, alkaloid and saponins), in vitro antioxidant activity (2, 2-diphenyl-1-picryl-hydrazyl-hydrate, metal chelating, ferric reducing, superoxide dismutase and total antioxidant activity) of different parts of Cyperus rotundus. Along with this, the extraction of polyphenolic compounds by conventional and non-conventional methods and their optimization by response surface methodology technique were also studied. As the outcomes of the study, maximum amount of total phenolics content was found in the ethanolic fraction of stem (37.88±0.94 mg gallic acid equivalent/g). The root has shown the highest flavonoid (0.24±0.03 mg QAE/g) and non-tannin content (21.55±2.43 mg gallic acid equivalent/g) in the extract of petroleum ether. The total alkaloids and tannin content in flower and root i.e., 5.05±0.08 mg caffeine equivalent/g and 76.28±0.62 mg tannic acid equivalent/g was found to be better in aqueous extract respectively. The fraction of ethyl acetate has reported the greatest saponin content (39.2±3.8 mg gallic acid equivalent/g). In case of flower part half-maximal inhibitory concentration value of 2, 2-diphenyl-1-picryl-hydrazyl-hydrate and superoxide dismutase antioxidant activity varies from 0.57-1.90 mg/ml and from 0.053-1.401 mg/ml respectively. Antioxidant activity of each part of the plant was reported in the fraction of ethanol and water by fluorescence recovery after photobleaching (0.403±0.009-0.886±0.02 mg/ml), metal chelation activity (0.010±0.0-0.306±0.001 mg/ml) and total antioxidant capacity (0.030±0.0-0.317±0.002 mg/ml). Microwave assisted extraction method was found to be the suitable one for optimization of total phenolic content. So, the relationship between different parameter was determined through response surface methodology technique and evaluated by plotting the graphs. Polar extracts have found to show the maximum quantity of secondary bioactive metabolites and antioxidant activity as compared to non-polar one. And among the comparison between conventional and non-conventional methods, ultra sonication (nonconventional method) has shown the highest yield of bioactive phenolic compound. So, this study shows that Cyperus rotundus has wide range of secondary metabolites showing antioxidant properties that can be applied for different therapeutic applications.

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Section: Resultssupporting

confidence: 82%

In Vitro Antioxidant Assay of Different Phytoconstituents and Extraction Optimization of Phenolics from Cyperus rotundus L

Varshney¹,

Prakash²,

Singh³

et al. 2023

IJPS

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“…This SI value was important because it indicates the safe value of plant extract for antiviral activity. A high value of SI indicated a large safety margin between beneficial and toxic doses [22]. As in the previous study, the antiviral activity with an SI value of more than 10 can be considered to possess a high potential for an antiviral agent [14].…”

Section: Antiviral Screeningmentioning

confidence: 52%

Evaluation of cytotoxicity and antiviral activity of Kyllinga nemoralis leaves and stems methanolic extract

Abd Wahab,

Mohd Saidi,

Rahman

et al. 2024

J App Biol Biotech

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This present study aimed to investigate the potential stems and leaves methanolic extract of Kyllinga nemoralis as an antiviral agent against Herpes Simplex Virus Type 1 (HSV-1). The leaves and stems of K. nemoralis methanolic extract were assessed in the cytotoxicity test using the MTT assays. The plaque reduction assay was evaluated in the post-treatment assay, pre-treatment assay, virucidal assay, time-addition test, and time-removal test. Cytotoxicity screening of stems and leaves of K. nemoralis against Vero cells shows that the cytotoxicity concentration of 50% of cell viability (CC50) values was 0.75 mg/mL, which indicates that K. nemoralis is non-cytotoxic and safe. The antiviral assay provides a Selective Index (SI) value (CC50/EC50) to determine the good SI for antiviral activity. In post-treatment, the SI value for this extract against HSV-1 was 8.33. In pre-treatment, the SI value for this extract was 21.43. In the virucidal test, the SI value for this extract was 24.19 mg/mL. In the time addition assay, the plaque inhibition reached 43% when the extract was added at 2 h.p.i, and it dropped to 15% at 10 h.p.i. In the time removal assay, the plaque inhibition reached 50% after the extract was removed at 2 h.p.i., and it increased to 87% at 24 h.p.i. This study showed that K. nemoralis has potential as a drug used to fight herpes infections.

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“…In comparison with some species of the genus Dialium, this activity appears to be much greater. Indeed, the antioxidant activity on DPPH, expressed as IC50 in μg / mL, of several species of the genus Dialium is reported in the literature, D. indum, 181.6 ± 0.4 [33,34], D. guineense, 50.23 ± 0.15 [35], D. corbisieri, 14.44 ± 0.12 and D. gossweileri, > 500 [36], D. cochinchinensis, 65.01 ± 0.12 [37]. This interesting antioxidant activity observed in D. angolense would be linked to the presence of phenolic compounds mainly flavonoids, identified and quantified within the plant during this study (table 2), as well as several previous studies which testify to the antioxidant activity of flavonoids [38][39][40][41][42].…”

Section: Discussionmentioning

confidence: 99%

In vivo antiplasmodial and toxicological studies of Dialium angolense Welw. Ex Oliv. (Fabaceae) leaves extracts, a medicinal plant from Eastern Congo

Valentin¹,

Salvius²,

Henry³

et al. 2020

World J. Bio. Pharm. Health Sci.

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Dialium angolense is used in traditional medicine in Bagira-Bukavu in the management of malaria. In this study, in vivo antiplasmodial and in vitro antioxidant activities, phytochemical screening of secondary metabolic and in vivo toxicological studies where carried out on aqueous and methanolic extracts of its leaves. The plant was selected following an ethnobotanical survey conducted in DR Congo and focusing on antimalarial plants. Extracts’ phytochemical secondary metabolites were determined using standard procedures and the antiplasmodial activity was evaluated using 4-day suppressive test, while antioxidant activity was evaluated by DPPH assay. In acute toxicity, eighteen animal (6/group) were given orally singular 2000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assay, 150 or 300 mg/kg BW/Day were given orally, and animals (6/group) were observed for 28 days. The total phenolic (0.89 - 0.98 mg GAEg-1), total flavonoid (0.42 - 0.44 mg QEg-1) and total tannin contents (0.080 – 0.098 mg GAEg-1) were in the same rate in the two extracts as well as the antioxidant activity with IC50 value 6.1 and 6.8 μg/mL. At the highest oral dose, 300 mg/kg body weight, all extracts produced 70.4–70.8% chemo-suppression against P. berghei ANKA and 28 survival days. No deaths were recorded during the acute toxicity assay suggesting the LD50 > 2000 mg/kg and no abnormal behavior or variation in toxicity biomarkers were observed during the subacute toxicity assessment. D. angolense leaves extracts showed a great antiplasmodial and a very good antioxidant activity. It can be used to prepare antimalarial recipe or isolate antimalarial compounds in the future.

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Evaluation of Antimicrobial, Antioxidant and Cytotoxic Activities of Dialium cochinchinensis Seed Extract

Cited by 8 publications

References 16 publications

In Vitro Antioxidant Assay of Different Phytoconstituents and Extraction Optimization of Phenolics from Cyperus rotundus L

In Vitro Antioxidant Assay of Different Phytoconstituents and Extraction Optimization of Phenolics from Cyperus rotundus L

Evaluation of cytotoxicity and antiviral activity of Kyllinga nemoralis leaves and stems methanolic extract

In vivo antiplasmodial and toxicological studies of Dialium angolense Welw. Ex Oliv. (Fabaceae) leaves extracts, a medicinal plant from Eastern Congo

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