Evaluation of Antimicrobial and Wound Healing Effects of Gold Nanoparticles Containing Abelmoschus esculentus (L.) Aqueous Extract

Abstract

Background. Wound healing is a complex process of replacing devitalized cellular structures and tissues with healthy cells and tissue. Nanotechnology has been increasingly proposed as a novel platform to treat wounds and skin regeneration. The aim of this study was to evaluate the antibacterial, antioxidant, cytotoxic, and cutaneous wound healing activities of phytosynthesized Au NPs using Abelmoschus esculentus (okra) and synthesized Au NPs by using the citrate synthesis method. The Ok Au NPs were characterized using various techniques like UV-Vis absorption spectroscopy, FTIR, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Cutaneous wounds were created on 30 rats and randomized into three groups: untreated and two groups treated with Ch Au NPs and Ok Au NPs. The treatment was carried out daily for 12 days. A peak characterized the Ok Au NPs at 538 nm in the UV-Vis spectrum. Based on the results of FTIR spectroscopy, various functional oxygenated groups such as hydroxyl, carboxyl, and nitrogenous groups were observed. XRD confirmed the crystalline nature of the nanoparticles. TEM images of Ok Au NPs showed a spherical shape and size range of 75 nm. DPPH test showed similar antioxidant potentials for Au NPs. The Au NPs showed cell viability in a dose-dependent manner, and this technique was found to be nontoxic. Agar well diffusion, which is the method to determine antibacterial characteristics of Au NPs, showed a significant beneficial effect against a variety of bacterial species. In addition, histopathological results showed that Au NPs could accelerate wound closure. Therefore, Au NPs could be suitable for wound healing applications.

Polyphenolic Compounds Nanostructurated with Gold Nanoparticles Enhance Wound Repair

“…Our findings are also in concordance with other reports that showed that gold nanoparticles capping with natural products, such as quercetin derived from Abelmoschus esculentus (L.) (okra), or with epigallocatechin gallate and α-lipoic acid on linear wound enhanced wound healing [6,27,28].…”

Cumin (Cuminum cyminum L.) seeds accelerates wound healing in rats: Possible molecular mechanisms

“…The microscopic observation of regenerated tissues, colored by H&E and Mason Trichoma stains, revealed noticeable tissue regeneration enhanced by the topical application of CCS, which was by the previous outcomes of wound‐curing effects of CCS extracts 24 . Similarly, researchers have shown the wound‐healing potentials of CCS obtained from a variety of medicinal plants 42–44 …”

“…24 Similarly, researchers have shown the wound-healing potentials of CCS obtained from a variety of medicinal plants. [42][43][44] The TGF-β1 is a well-known multifunctional polypeptide factor considered an important mediator of tissue regeneration (angiogenesis) and faster wound repair because of its modulatory action on…”

“… 24 Similarly, researchers have shown the wound‐healing potentials of CCS obtained from a variety of medicinal plants. 42 , 43 , 44 …”

Retracted: Evaluation of Antimicrobial and Wound Healing Effects of Gold Nanoparticles Containing Abelmoschus esculentus (L.) Aqueous Extract

“…This article has been retracted by Hindawi, as publisher, following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of systematic manipulation of the publication and peer-review process.…”