Evaluation of Antimicrobial Activity of Plants Extract Against Bacterial Pathogens
Isolated from Urinary Tract Infection among Males Patients

Ahmed, Mais E.; Al-lam, Makarim Q.; Ali, Dina

doi:10.33091/amj.0701622020

Cited by 4 publications

(3 citation statements)

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“…These results are consistent with (Ahmed & AL-Shimmary, 2018), the observations found the highest activity from E. faecalis was observed after a three-day incubation period. Many factors underlie their antimicrobial activity, including the fact that, in contrast to antibiotics, they contain distinct functional groups and that, as a result, bacteria are less resistant to bacteriocin and plant antimicrobials (Ahmed et al, 2021). The agar well diffusion assay was used to measure the antibacterial activities of the bacteriocin, which produced a 23 mm inhibition zone diameter at a pH of 5 (Kasimin et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Enterococcus faecium BACTERIOCIN EFFLUX PUMP MexA GENE AND PROMOTE SKIN WOUND HEALING IN MICE

Ahmed,

Al-Awadi

2024

J microb biotech food sci

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The process involved isolating E. faecium from the gut of honeybees, screening the bacterium for bacteriocin-like inhibitory substance (BLIS), evaluating its impact on the expression of the mexA gene in multidrug-resistant (MDR) P. aeruginosa, and determining the role of bacteriocin in treating infected wounds in mice through histopathological examination. After evaluating the best circumstances for producing BLIS, it was discovered that glucose was a superior carbon source and yeast extract was the best source of nitrogen. The pH was found to be 5, the ideal incubation time was 72 hours, and ammonium sulfate salt was used for partial purification at 80% saturation. The identification of MDR P. aeruginosa isolates from pus infections was a further focus of the study. The VITEK 2 system was used to perform the identification. The results of antibiotic susceptibility tests revealed that the greatest resistance rates were found against Meropenem (83.3%) and Gentamicin (73.3%), followed by beta-lactam antibiotics (Ticarcillin, Ticarcillin/Clavulanic Acid, Piperacillin, and Aztreonam), which showed resistance in about 66.6 and 36.6% of the study isolates, respectively. Followed by Imipenem (63.3%), Ceftazidime (36.6%), and Cefepime (36.6%). The mexA gene was detected in all nine strains. The study also investigated the impact of the bacteriocin of the chosen strain on the expression of the mexA gene. An in vivo study revealed that wound healing was enhanced by treating infected wounds with E. faecium bacteriocin. Conclusion: Down-regulation and up-regulation in the expression of the genes following exposure to Bacteriocin indicate the potential of E. faecium as an effective antimicrobial agent against MDR P. aeruginosa infections.

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Section: Discussionmentioning

confidence: 99%

Enterococcus faecium BACTERIOCIN EFFLUX PUMP MexA GENE AND PROMOTE SKIN WOUND HEALING IN MICE

Ahmed,

Al-Awadi

2024

J microb biotech food sci

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“…VRSAcin antibacterial activity was tested using the Agar Well Diffusion (AWD) method on Muller-Hinton agar. [17]…”

Section: Vrsacin Activity Assaymentioning

confidence: 99%

Antibacterial and therapeutic effects of Vancomycin-resistant Staphylococcus aureus bacteriocin (VRSAcin) in treatment of VRSA skin infection in mice

qassam,

emad

2024

Preprint

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Vancomycin Staphylococcus aureus (VRSA) is a strain belonging to S. aureus that is considered the main cause of bacterial skin and soft tissue infections. It has acquired resistance to vancomycin and represents a therapeutic challenge. The current study aimed to compare the possible therapeutic effects of VRSA bacteriocin (VRSAcin) on the treatment of skin infection in mice compared with an antibiotic (linezolid). The results showed that from fifty swabs obtained from human skin wounds, only 30 samples were identified as Staphylococcus spp., and 20 samples of them were identified as VRSA strains. One isolate was selected for VRSAcin extraction depending on its antibiotic resistance using an antibiotic susceptibility test. The typical conditions for the production of VRSAcin include pH 7 and a temperature of 37°C for 48 hours. In mice, VRSA-contaminated wounds revealed severe tissue distraction and inflammation that extended to the hypodermis, while VRSA-treated skin showed mild changes and localized lesions to the epidermis and upper dermis. Linezolid-ointment-treated skin shows moderate to severe changes. In conclusion, VRSA strain infections in human burned skin were found to be more common than expected. In vivo studies in mice indicated that wounded skin infected with VRSA can be treated with VRSAcin as an antibacterial agent that promotes healing processes with obvious superiority to linezolid ointment.

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“…All of them were collected from September 2022 to February 2023 from patients who were referred to Baghdad Medical City and Al-Yarmouk Teaching Hospitals. Th e samples were collected using sterile cotton swabs, then kept in a cool place until transported to the laboratory, then cultured on Cetrimide and Mac-Conkey agar and incubated for 24 hr at 37 °C (Ahmed et al, 2021).…”

mentioning

confidence: 99%

Синергічний вплив гентаміцину та наночасток оксиду заліза на ген phzM синьогнійної палички

Ahmed,

Abdul Muhsin

2024

Mikrobiol. Z.

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The increased use of iron-containing nanoparticles and green synthesis nanoparticles is beneficial and less harmful to the environment and human health. Working antimicrobial peptide functions by inhibiting the virulence factor of Pseudomonas aeruginosa, which is the essential mechanism of drug resistance by possessing an efflux pump important virulent factor. The main aim of the research was to study the effects of synergistic action of gentamicin (GNT) and iron nanoparticles (IONps) to solve the problem of multidrug-resistant P. aeruginosa. Methods. The isolation and identification of multidrug-resistant P. aeruginosa from burns and wound infections were carried out using the VITEK 2 system, and the phzM genes were detected in all the strains. The effectiveness of IONps and their role in reducing phzM gene expression were investigated. Results. The characterization of IONps biologically manufactured using a wavelength spectrum UV-vis spectrophotometer (maximum peak at 600 nm) and tests of atomic force microscopy showed that their diameter reached 34 nm. An electron microscope examination revealed that the produced particles were spherical, indicating good homogeneity and quality of the IONps. The results showed significant down-regulation changes in phzM expression after treatment with GNT and IONps. The result of qRT-PCR in this study revealed that the fold change in the expression of the phzM gene was downregulated in response to the IONps (8µg/mL) + GNT (16µg/mL) combination. Conclusions. One of the justifications for using P. aeruginosa in preparing IONps is that this bacteria needs iron as a growth promoter, which helps in the formation of IONps. The inhibitory effect on the efflux pump gene expression may represent a potential strategy for controlling P. aeruginosa infections.

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Evaluation of Antimicrobial Activity of Plants Extract Against Bacterial Pathogens Isolated from Urinary Tract Infection among Males Patients

Cited by 4 publications

References 0 publications

Enterococcus faecium BACTERIOCIN EFFLUX PUMP MexA GENE AND PROMOTE SKIN WOUND HEALING IN MICE

Enterococcus faecium BACTERIOCIN EFFLUX PUMP MexA GENE AND PROMOTE SKIN WOUND HEALING IN MICE

Antibacterial and therapeutic effects of Vancomycin-resistant Staphylococcus aureus bacteriocin (VRSAcin) in treatment of VRSA skin infection in mice

Синергічний вплив гентаміцину та наночасток оксиду заліза на ген phzM синьогнійної палички

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