Evaluation of Antimalarial Activity of the Leaf Latex and TLC Isolates from<i> Aloe megalacantha</i> Baker in<i> Plasmodium berghei</i> Infected Mice

Hintsa, Gebretsadkan; Sibhat, Gereziher; Karim, Aman

doi:10.1155/2019/6459498

Cited by 23 publications

(25 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative variation in parasite suppression activity among doses might be due to variation in the amount of secondary metabolites contents in the leaf latex of Aloe weloensis. This show that Aloe weloensis is endowed with a very good antimalarial activity and concordant to promising antimalarial activity of Aloe vera, Aloe pulcherrima and Aloe megalacantha in the genus Aloe (19)(20)(21). In another studies, signi cant dose-dependent parasite suppression was demonstrated after administration of Aloe macrocarpa, Aloe debrana and Aloe sinana which indicate a promising antimalarial activity and providing the scienti c evidence for the folkloric use of the plant (41,42).…”

Section: Discussionsupporting

confidence: 54%

“…The latex of Aloe weloensis leaf showed antibacterial effect against gram negative and gram positive strains (17). The leaf latex of the plant has been used in folk medicine against malarial and others human ailment in Ethiopia (18).The leaf latex and isolated compounds of Aloe vera, Aloe pulcherrima and Aloe megalacantha, a similar plant species, possessed signi cant antimalarial activity (19)(20)(21).As a result, Aloe weloensis may have similar effect as all plants belong to the same genus. Therefore, the current study was aimed to investigate antimalarial activity of the leaf latex of Aloe weloensis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

Amare

Awgichew

Ahmed

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Nature has gifted a variety of plants having potential effect against plasmodium parasites. The present study was aimed to determine in vitro and in vivo antimalarial activity of the leaf latex of Aloe weloensis.Methods: In vitro antimalarial activity of the leaf latex of A. weloensis was determined against 3D7 strain of P. falciparum. Antimalarial activity of the three doses the latex was evaluated in 4 day-suppressive and curative models against P. berghei infected mice. Antioxidant activity of the leaf latex of A. weloensis was assessed in 2,2- diphenyl 1- picrylhydrazine assay model. Results: Antioxidant activity of the latex was concentration dependent; the strongest inhibition was measured at 400 μg/mL (73.54%). The leaf latex of A. weloensis was demonstrated inhibitory activity against 3D7 malarial strain (IC50 = 9.14 μg/ml). Suppressive and curative effect of the latex was found to be dose dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in 4-day suppressive test compared to vehicle control. Parasitemia level of the mice treated with 200, 400 and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58% and 64% respectively in curative test. Administration of the leaf latex of A. weloensis significantly (p<0.01) improved mean survival time, pack cell volume, rectal temperature and body weight of P. berghei infected mice. Conclusion: The finding showed that the leaf latex of Aloe weloensis endowed prominent antimalarial and antioxidant activities. The result can serve as a step towards the development of safe and effective herbal therapy against plasmodium parasites.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

Amare

Awgichew

Ahmed

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In Ethiopia there are around 46 species of Aloe, of which about 66% are endemic in the country [8]. Several of these endemic species possessed different biological activities, including antimicrobial [9,10], antitrypanosomiasis [11], antileishmaniasis [12,13], antimalarial [14][15][16], wound healing [17], anti-inflammatory [17,18], anticancer [19], antidiabetic [20], and antioxidant activities [21]. is relevance indicates that Aloe is the store house of numerous bioactive compounds [22].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Evaluation of Latex and TLC Fractions from the Leaves of Aloe adigratana Reynolds

Asmerom

Hailu

Yimer

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background. The highest prevalence and emergence of microbial infections coupled with the threat of antimicrobial resistance constitute a global concern, which entails searching for novel antimicrobial agents. Medicinal plants are among the major sources of medicines for novel drug discovery. Aloe adigratana is one of the endemic Aloe species in Ethiopia where the leaf latex of the plant is traditionally used for the treatment of various pathogenic conditions such as wound, dandruff, malaria, and diabetes. In spite of such claims, there was no scientific study done so far. The aim of the current study was, therefore, to evaluate the antimicrobial effect of leaf latex of A. adigratana and its thin layer chromatography (TLC) fractions. Methods. Thin layer chromatography (TLC) separation was employed for isolation of bioactive compounds. Agar well diffusion and microdilution assay method were used to evaluate the antimicrobial actions of the leaf latex and TLC fractions against six bacterial strains and four Candida species of reference and clinical isolate microbial strains. Results. Three major fractions, AA01, AA02, and AA03, were identified by TLC. Among the tested microbial strains, the reference strain of Staphylococcus aureus ATCC 29213 (MIC = 0.06 mg/mL) and clinical Candida krusei 242/18 (MIC = 0.14 mg/mL) exhibited higher susceptibility towards AA02, while reference strains of Klebsiella pneumoniae ATCC 700603 (MIC = 0.19 mg/mL) revealed the highest susceptibility towards AA01. The leaf latex displayed the highest activity against Staphylococcus aureus ATCC 29213 and clinical Candida krusei 242/18 with a MIC value of 0.19 mg/mL. Conclusion. The leaf latex and TLC fractions were found to be active against the tested bacterial and Candida species. Therefore, this finding supports the traditional claim of Aloe adigratana and the need for characterization of the TLC fractions to provide as lead compounds for further comprehensive antibacterial and antifungal activities.

show abstract

“…The leaf latexes of both A. elegans and A. monticola were collected and dried as per the method described and were considered as an extract [1,13]. The leaf of H. schulli and the roots of C. tomentosa and T. gracilipes were dried under shaded open air.…”

Section: Preparation Of the Extractsmentioning

confidence: 99%

“…Beyond 80% of the global population are heavily using traditional medicines, commonly plant-based medicines, for management of numerous types of illnesses including malaria [1]. Malaria is an infectious disease caused by the parasites belonging to Plasmodium genus with P. falciparum causing the most life-threatening type of malaria [2].…”

Section: Introductionmentioning

confidence: 99%

In vitro antimalarial activity of selected medicinal plants native to Tigray region of Ethiopia

Tekulu¹,

D’Alessandro²,

Parapini³

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

BackgroundVarious medicinal plants are used as alternative remedies for the management of malaria, an important infectious disease responsible for around 228 million cases and 405,000 deaths worldwide in 2018. The worsening of the disease is highly associated to an emergence of drug-resistant parasites. Hence, the search of new alternative antimalarial agents from traditionally used medicinal plants is the most viable approach. The current study was aimed to evaluate the in vitro antiplasmodial property of Aloe elegans, Aloe monticola, Capparis tomentosa, Hygrophila schulli and Tephrosia gracilipes extracts.Methods Leaf latexes were collected from Aloe elegans and Aloe monticola, whereas cold maceration with 70% ethanol was used to prepare extracts from roots of Capparis tomentosa and Tephrosia gracilipes , and leaves of Hygrophila schulli . The antiplasmodial activity of the extracts against asexual and gametocyte stages was tested using parasite lactate dehydrogenase assay and luciferase assay, respectively. Cell cytotoxicity was assessed on human microvascular endothelial cells by the MTT assayResults Of the five selected medicinal plants, leaf latex of Aloe monticola showed the best activity against both asexual stages and stage V gametocytes of Plasmodium falciparum . The leaf latex of Aloe elegans and ethanolic extract of Hygrophila schulli leaves also showed antiplasmodial property against asexual stages. On the contrary, the roots of Capparis tomentosa and Tephrosa gracilipes were inactive.Conclusion Findings of this study may partly support the acclaimed traditional use of the leaves latexes of both Aloe elegans and Aloe monticola and the ethanolic extract of Hygrophila schulli leaves for the management of mild to moderate malaria.

show abstract

Evaluation of Antimalarial Activity of the Leaf Latex and TLC Isolates from Aloe megalacantha Baker in Plasmodium berghei Infected Mice

Cited by 23 publications

References 34 publications

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

Antimicrobial Evaluation of Latex and TLC Fractions from the Leaves of Aloe adigratana Reynolds

In vitro antimalarial activity of selected medicinal plants native to Tigray region of Ethiopia

Contact Info

Product

Resources

About