Evaluation of Antiherpetic Compounds Using a Gastric Cancer Cell Line: Pronounced Activity of BVDU against Herpes Simplex Virus Replication

Kodama, Eiichi; Igarashi, Akira; Mori, Shuichi; Hashimoto, Kohichi; Suzuki, Toshimitsu; Clercq, Erik De; Shigeta, Shirô

doi:10.1111/j.1348-0421.1996.tb01080.x

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…Each assay was performed in duplicated and repeated three times. The antiamoebic activity was also evaluated by MTT assay [31]. After incubation of parasites with chromone-tetrazoles as described above, the trophozoites were washed and incubated for 45 min at 37 °C in a saline phosphate buffer with 0.5 mg/mL of MTT (3-[4,5-dimethylhiazol-2-il]-2,5-diphenyl tetrazolium bromide) (Sigma).…”

Section: Methodsmentioning

confidence: 99%

In Vitro Studies of Chromone-Tetrazoles against Pathogenic Protozoa, Bacteria, and Fungi

et al. 2015

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Abstract:In vitro studies to fourteen previously synthesized chromone-tetrazoles and four novel fluorine-containing analogs were conducted against pathogenic protozoan (Entamoeba histolytica), pathogenic bacteria (Pseudomonas aeruginosa, and Staphylococcus aureus), and human fungal pathogens (Sporothrix schenckii, Candida albicans, and Candida tropicalis), which have become in a serious health problem, mainly in tropical countries.

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Section: Methodsmentioning

confidence: 99%

In Vitro Studies of Chromone-Tetrazoles against Pathogenic Protozoa, Bacteria, and Fungi

et al. 2015

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“…After antiviral therapy, immunosuppressants were reduced, and the CMV gastric polyp volume dropped (Wong, Tan, Leow, & Mesenas, 2018). Some experience also showed the effect of the antiviral drug, brivudine on MKN-28, a herpes simplex virus-positive gastric cancer cell line (Kodama et al, 1996). There are a limited number of types of cancer associated with human immunodeficiency virus (HIV) that were reported to date, but some evidence showed that people infected with HIV have a high venture of some carcinoma such as gastric cancer (Shiels, Cole, Kirk, & Poole, 2009 (Borges et al, 2016).…”

Section: Quercetinmentioning

confidence: 99%

Infection‐associated epigenetic alterations in gastric cancer: New insight in cancer therapy

Fattahi

Kosari-Monfared

Ghadami

et al. 2018

Journal Cellular Physiology

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Gastric cancer risk is higher for malignancies motivated by bacterial and viral infections. Epigenetic abnormalities including DNA methylation, histone modifications, and noncoding RNAs are important regulatory key players in gastric cancer development in infected patients. Epigenetic memory restoration is an extremely interesting phenomenon which should be considered in therapeutic approaches. In vitro and in vivo antiviral treatments in combination with epigenetic therapeutic strategies along with standard chemotherapy revealed promising outcomes in gastric cancer prevention and treatment. This review summarizes our current understanding of the gastric cancer infections and epigenetic alterations caused by these agents. We focus on studies highlighting recent advances in epigenetic restoration by target specific drugs and present also a comprehensive overview of effective antiviral drug treatments against gastric cancer.

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“…Antiviral activity by viral plaque formation number reduction assay: This assay followed the procedures previously described [31], with minor modifications. Vero cells (3 × 10 5 cells per well) were seeded onto 24-well culture plates, and after 24 h, the cells were infected with 100 PFU of HSV-1.…”

Section: Evaluation Of Cytotoxicity By Mttmentioning

confidence: 99%

Antiviral Activity of Euphorbia Lectin Against Herpes Simplex Virus 1 and its Antiproliferative Activity Against Human Cancer Cell-Line

Torky¹

2016

J Antivir Antiretrovir

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Lectin is a protein that specifically bound and cross-linked with carbohydrates and is involved in the activation of the lectin pathway which is considered a part of both innate and adaptive immunity. Lectin also has a role in plant defense system. In this study, lectin was isolated from Euphorbia and purified through aqueous extraction, ammonium sulfate precipitation, dialysis, filtration on superdex 75, ion exchange chromatography on SP-sepharose gel. The study showed that lectin exhibits hemagglutination activity towards rabbit erythrocytes on concentration 50 µg/ml. This hemagglutination activity was retained by lectin till 60°C. Effect of pH, and denaturalizing agent on lectin was also studied, and found to have a low pH range (6-8). Molecular weight was determined for lectin using SDS gel-electrophoresis and found to be 30 KD, and determined by gel filtration to be 60 KD, indicating that lectin is homodimer in the plant. Cytotoxicity assay of Euphorbia on the vero cell lines using MTT, was also studied and revealed that using a concentration till 100 µg/ml of Euphorbia was within the safe limit. Also the determination of antiviral activity of the Euphorbia lectin against HSV1 on vero cell lines was investigated using plaque reduction assay and MTT, showing 76% of virus inhibition. Mechanism of action of the effect of lectin on HSV1 was also investigated using MTT, and RT-PCR. The study also showed that lectin affected the virus entry and attachment phase. This inhibition of the virus was totally diminished when the lectin was combined with EDTA before infection with HSV1. Antiproliferative activity of Euphorbia was also studied against human cancer cell line by MTT assay. The gene(s) encoding the lectin was isolated by PCR, which revealed that lectin is actually encoded by multi gene family.

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Evaluation of Antiherpetic Compounds Using a Gastric Cancer Cell Line: Pronounced Activity of BVDU against Herpes Simplex Virus Replication

Cited by 7 publications

References 16 publications

In Vitro Studies of Chromone-Tetrazoles against Pathogenic Protozoa, Bacteria, and Fungi

In Vitro Studies of Chromone-Tetrazoles against Pathogenic Protozoa, Bacteria, and Fungi

Infection‐associated epigenetic alterations in gastric cancer: New insight in cancer therapy

Antiviral Activity of Euphorbia Lectin Against Herpes Simplex Virus 1 and its Antiproliferative Activity Against Human Cancer Cell-Line

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