Evaluation of antigout activity of Phyllanthus emblica fruit extracts on potassium oxonate-induced gout rat model

Abstract

Aim:The present study has been conducted to evaluate antigout activity of aqueous and alcoholic extracts of Phyllanthus emblica fruits following its 28 days repeated oral administration on potassium oxonate-induced gout rat model.Materials and Methods:The study was conducted on 42 male Sprague-Dawely rats dividing them in seven groups having six rats in each group. Groups I, II, and III served as vehicle control group, gout control group, and standard treatment control group, respectively. Rats of all the groups except vehicle control group were administered potassium oxonate at 250 mg/kg (IP), throughout the study period (28 days) for induction of gout. Groups IV and V received aqueous extract of P. emblica at 200 and 400 mg/kg, and Groups VI and VII received alcoholic extract of P. emblica at 200 and 400 mg/kg (daily oral for 28 days). At the end of study, all the rats were subjected to blood collection; blood and serum sample were analyzed for hematological and biochemical parameters, respectively. After collection of blood samples on the 29th day, all the rats were sacrificed and subjected to post mortem examination to determine the presence or absence of gross and histopathological lesions in kidney tissues.Results:At the end of study, rats of gout control group showed increase in platelets counts, serum creatinine, uric acid, blood urea nitrogen (BUN), and xanthine oxidase (XO) enzyme level along with alterations in kidney tissues as compared to vehicle control group. Gouty rats treated with aqueous and alcoholic extracts of P. emblica at 200 and 400 mg/kg body weight and standard treatment allopurinol at 5 mg/kg body weight showed reduction in platelets counts, serum creatinine, uric acid, BUN, and XO enzyme level along with significant improvements in histological structure of kidney as compared to rats of gout control group.Conclusion:Oral administration of aqueous and alcoholic extracts of P. emblica fruits for 28 days has shown protection against gout in dose-dependent manner in rats.

Protective Effect of Emblica officinalis on Cardiac Dysfunction in Gamma-Irradiated Male Albino Rats

“…These present results are consistent with other findings [29]. Elevation in xanthine oxidoreductase activity and lowering in superoxide dismutase activity, were observed in the intestine of mice exposed to whole-body gamma irradiation, which, however, reverted back to those levels of controls, when animals were fed triphala (a herbal formulation containing EO fruits) for 7 days prior to irradiation.…”

Anti-gout Potential of Malaysian Medicinal Plants

“…Several in vivo studies have demonstrated a reduction of serum uric acid levels in hyperuricemic rats. For example, administration of aqueous and alcoholic extracts of Phyllanthus emblica (200 and 400 mg/kg) reduced serum uric acid and xanthine oxidase enzyme levels in hyperuricemic rats while allopurinol was more potent in inhibiting xanthine oxidase enzyme (Sarvaiya et al, 2015 ). Similar results have also been reported by El-Rahman and Abd–Elhak ( 2015 ) for Allium ampeloprasum, Apium graveolens , and P. crispum using albino rats, where both extracts significantly reduced serum uric acid and lipid peroxidation and increased antioxidant enzyme activity levels at a dose of 5 g/kg.…”

Toxicological Evaluation of Saposhnikoviae Radix Water Extract and its Antihyperuricemic Potential

“…The maximal treatment dose of SRE in mice was set at 500 mg/kg, which showed a remarkable reduction in serum uric acid in male rats. Potassium oxonate was employed to induce gout mediated by the inhibition of uricase, according to a previous report (18). Here, we used male mice to investigate antihyperuricemia, because endogenous reproductive hormones during the menstrual cycle largely affect uric acid levels (24).…”

“…Although allopurinol, a XO inhibitor, and benzbromarone, an inhibitor of protein transporter of urate (URAT1) are considered safe first-line drugs in gout, side effects such as liver toxicity, gastrointestinal disorders, and skin rash can occur (17). To describe the potential of SRE against hyperuricemia, the effect of SRE on serum UA, serum creatine, urinary UA, and urinary XO activity were determined in potassium oxonate (PO)-induced gout mice (18,19). In addition, the histological evaluation of the kidney was conducted to identify the beneficial role of SRE on PO-induced kidney injury in mice.…”