Evaluation of Antidiabetic and Antioxidant Properties ofBrucea javanicaSeed

Abstract

The ethanol extract of B. javanica seed was fractionated with solvents of different polarities and tested for antioxidant activities by several assays including DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP), ferrous ion chelating activity (FCA), and nitric oxide radical scavenging activity (NORSA) along with their polyphenolic contents. Antidiabetic activity was evaluated both in vitro and in vivo using a glycogen phosphorylase α (GPα) inhibition assay and oral glucose tolerance test (OGTT) in nondiabetic rats. The ethyl acetate fraction (EAF), rich in tannin, exhibited the strongest antioxidant activities to DPPH, FRAP, and NORSA, except for FCA. The EAF also exerted a dose-depended inhibition of GPα (IC50 = 0.75 mg/ml). Further evaluation of hypoglycemic effect on OGGT indicated that rats treated with EAF (125 mg/kg bw) showed a 39.91% decrease (P < 0.05) in blood glucose levels at 30 min, and continuous fall (P < 0.05) of 28.89% and 20.29% was observed in the following hours (60 and 90 min) compared to the normal control during OGTT. The EAF was applied to polyamide column chromatography, and the resulting tannin-free fraction was tested for both GPα inhibition and antioxidant (DPPH only) activity. The GPα inhibitory activity was retained, while antioxidant activity was lost (4.6-fold) after tannin removal. These results concluded that the GPα inhibitory activity initially detected was primarily due to the compounds other than tannins, whereas antioxidant activity was mainly due to the tannins.

In vitro antioxidant and in vivo xanthine oxidase inhibitory activities of Pandanus amaryllifolius in potassium oxonate‐induced hyperuricemic rats

“…This supports the study by Ablat et al . () as they found that the amount of TPC increased as the polarity of the solvent. According to Kim et al .…”

“…Thus, this showed that extraction using different solvents leads to different capability of antioxidant scavenging activity due to varying phytochemical composition present in each extract. Few other studies also proved a significant correlation between TPC and DPPH assay (Ng et al ., ; Tiveron et al ., ; Ablat et al ., ; Saha & Verma, ).…”

“…The total phenol content and total flavonoid content of P. amaryllifolius were determined as described by Ablat et al (2014). In a 96-well microplate, 20 lL leaf extract was mixed with 100 lL of Folin-Ciocalteu reagent and incubated for five minutes.…”

EFEK HIPOGLIKEMIK INFUSA DAUN TANAMAN MAKASAR, <em>Brucea javanica</em> (L) Merr, PADA TIKUS HIPERGLIKEMIK YANG DIINDUKSI ALOKSAN

“…Menurut International Diabetes Federation (IDF), sekitar 285 juta orang terkena diabetes di dunia dan diperkirakan jumlah ini meningkat menjadi 438 juta pada tahun 2030 (Chackrewarthy & Tahbrew, 2014). Komplikasi DM menyebabkan lebih dari 1 juta orang diamputasi, setengah juta mengalami gagal ginjal, dan 1,5 juta orang mengalami kebutaan setiap tahunnya.…”

“…Salah satu tumbuhan di Pulau Lombok yang diketahui memiliki aktivitas antidiabetes yang cukup tinggi berdasarkan pengalaman empiris masyarakat di daerah Sesaot (Kecamatan Narmada, Kabupaten Lombok Barat) adalah biji buah makasar (Brucea javanica (L.) Merr) (Okuyama et al, 1990). Tanaman ini dikenal juga dengan "Tanaman Wali" karena dipercaya memiliki aktivitas antidiabetes (Ablat et al, 2014), disamping aktivitas lainnya seperti antikanker (Sakaki, et al, 1986) dan antimalaria (Hout et al, 2006). Ablat et al, (2017) melaporkan fraksi etanol dan etil asetat ekstrak etanol biji buah makasar dapat menurunkan kadar gula darah mencit diabetes dan menghambat aktivitas enzim α-glukosidase.…”