Evaluation of Antibiotic Resistance and Biofilm Formation Ability Uropathogenic E. coli (UPEC) Isolated From Pregnant Women in Karaj

Nikzad, Maryam; Mirnejad, Reza; Babapour, Ebrahim

doi:10.30699/ijmm.15.2.195

Cited by 6 publications

(5 citation statements)

References 27 publications

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“…The current study result was relatively similar to the study conducted in Iran, which showed that there was no statistically significant relationship between antibiotic susceptibility and biofilm formation; although biofilm production increases antibiotic susceptibility in bacteria, drug resistance does not depend only on the presence of biofilm and many other factors such as the presence of degrading enzymes, the presence of effusion pumps, and changes in the site of action 3 . Furthermore, the study was contrary to the Ugandan study findings which demonstrated a significant association between antibiotic susceptibility and biofilm formation of E. coli.…”

Section: Association Between Antibiotic Susceptibility and Biofilmfor...supporting

confidence: 84%

“…41 Likewise, a study conducted in Iran, showed that 48.4% of the E. coli isolates were strong biofilm formers, 15.6% were moderately potent, 21.8% were weak and 14.2% were not biofilm formers. 3 These differences may be due to having a high number of E. coli examined compared to that of the current study.…”

Section: Susceptibility Patterns Of E Coli Isolated From the Urine Of...mentioning

confidence: 79%

“…2 Escherichia coli (E. coli) is the most common cause of (UTIs) in humans, among the factors, due to its ability to attach to epithelial cells, urinary lavage resistance, and biofilm formation. 3 In SSA, UTIs are among the most common infections with limited microbiological data to guide treatment decisions, 4 and have high frequency compared to developed countries. 2 A study conducted in Cameroon showed the prevalence of UTIs caused by E. coli to be 38% in pregnant women.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Biofilm Formation and Antibiotic Susceptibility in Escherichia coli Isolated from the Urine of Pregnant Women at Mnazi Mmoja Hospital, Zanzibar, Tanzania

2024

EASci

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Background: Escherichia coli is one of the species mostly involved in biofilm formation, being the most important cause of relapse or chronic urinary tract infections. To develop alternative biofilm-fighting treatments, it is important to understand which types of E. coli form biofilms. Study objectives: To detect biofilm formation and antibiotic susceptibility among E. coli isolated from the urine of pregnant women at

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Section: Association Between Antibiotic Susceptibility and Biofilmfor...supporting

confidence: 84%

Section: Susceptibility Patterns Of E Coli Isolated From the Urine Of...mentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Biofilm Formation and Antibiotic Susceptibility in Escherichia coli Isolated from the Urine of Pregnant Women at Mnazi Mmoja Hospital, Zanzibar, Tanzania

2024

EASci

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“…Another study by Nikzad et al found that 85.8% of UPEC strains were both strong and weak biofilm makers [ 50 ]. Biofilm generation was estimated to be 62.5% in a study shown by Katongole et al in 2020 on 200 UPEC isolates [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity, Carbapenem Resistance Genes, and Biofilm Formation in UPEC Isolated from Patients with Catheter-Associated Urinary Tract Infection in North of Iran

Nasrollahian

Halaji

Hosseini

et al. 2022

International Journal of Clinical Practice

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Background. Infections due to carbapenem-resistant Enterobacteriaceae (CRE) are associated in patients with urinary catheters alarming rate of emergency status. e aim of this study is to investigate the molecular causes of carbapenem resistance among UPEC as well as antimicrobial resistance trends. Additionally, the potential of isolates to produce biofilms, in addition to their clonal and genetic diversity, was investigated. Material and Methods. A cross-sectional study was accomplished on a collection of 76 non-duplicate UPEC isolates obtained from CAUTIs from May 2021 to September 2021. e modified carbapenem inactivation method (mCIM) and EDTA-modified carbapenem inactivation method (eCIM) test was performed for the detection of carbapenemase and metallo-beta-lactamase activity. Also, the presence of carbapenemase genes was determined using PCR assays. In 96-well microtiter plates, biofilm development was evaluated. ERIC-PCR was used to investigate the clonal and genetic variety of isolates. Results. A total of 76 confirmed UPEC isolates were obtained from patients mentioned to teaching hospitals in Babol, Iran. e results of antibiotic susceptibility testing revealed a high rate of antibiotic resistance against nalidixic acid (81.6%) and trimethoprim-sulfamethoxazole (80.3%). Among UPEC isolates, 63.2% and 13.2% of UPEC isolates were positive for MBL production. e frequencies of the studied genes are in order of bla NDM (14.5%), bla OXA-23 (2.6%), and bla OXA-48 (2.6%). Forty-two isolates (55.3%) were positive for biofilm formation. ERIC-PCR revealed that UPEC isolates could be categorized into nine clusters A-I and five isolates were categorized as a singleton. Conclusion. e high prevalence of MDR and carbapenemaseproducing isolates among the UPEC strain in this investigation is concerning. Moreover, the bla NDM was the most frequent cause of producing metallo-beta-lactamase and carbapenemase. Also, analysis revealed a partial genetic similarity among the studied isolates, indicating that the same UPEC clones may have spread to other hospital units.

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“…Furthermore, burns unit overcrowding is a significant source of cross infection, necessitating constant monitoring of bacterial species and drug susceptibilities. This is required because significant changes in these data may be associated with changes in clinical treatment in terms of drug selection for therapy [6]. Pathogenic bacteria have a variety of virulence characteristics that allow them to colonize, invade, and infect their hosts [5].…”

Section: Issn: 2583-4053mentioning

confidence: 99%

Molecular Analysis of Some Virulence Genes of Escherichia Coli Isolates from Wound and Burn Samples in Kirkuk City

Magthab

2023

J. Res. Appl. Sci. Biotechnol.

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Background: Gram-negative infections caused by non-spore producing E. coli that are facultatively anaerobic have been recognized as serious infections linked to higher mortality and significant costs associated with medical care. Aim: The goal of this study was to determine whether or not a number of E. coli strains are capable of producing virulence genes by using a technique called real time PCR. Materials and Methods: 155 people with burns and wounds had their cultures obtained, and susceptibility testing using swabs, tissue biopsies, and needle aspirations were performed. When compared to other techniques of specimen collection, swab cultures yield precise results and are a practicable, widely utilized, non-invasive, and cost-effective method. The two most recommended techniques for obtaining specimens are tissue biopsy and needle aspiration. Swab cultures, on the other hand, are appropriate since they are useful, widely applied, non-invasive, and reasonably priced. Samples from patients were collected in the consultation rooms, including swabs from burns and wounds. The sufferers had these taken from them. Before being identified, smear samples were initially cultivated in the lab on several selective media. Transport media-filled sterile collection containers containing these samples were brought to the lab. Results: According to the study's findings, E. coli was found in 27.10% of the burns and wounds samples. According to the study's findings, the majority of patients with E. coli infections (71.43 percent) were females, while only 28.57 percent were males. According to the study's findings, 28.57 percent of patients diagnosed with E. coli infections were between the ages of 31 and 40. The investigation also indicated that all of the sputum samples containing E. coli isolates were from patients above the age of 40. Furthermore, 45 percent of urine samples were collected from patients aged 31 to 40, and 42.86 percent of patients had wound infections. When examined using the MTP technique, fifty percent of the E. coli isolates tested positive for being potent biofilm producers, according to the study's findings. The DNA of 12 distinct E. coli bacterial isolates was isolated and purified using a genomic DNA purification kit. The bacteria were cultured on chromogenic agar medium to produce these E. coli isolates. The results were detected using electrophoresis on agarose gel at a concentration of 1.5 percent. The gel was then exposed to ultraviolet light, which revealed the DNA as compact bands. The virulence gene was found in 95.24 percent of the E. coli isolates taken from burn and wound sites, according to the study's findings. The majority of E. coli isolates tested positive for amikacin, imipenem, and gentamycin, according to the study's findings. The majority of E. coli isolates, on the other hand, were resistant to the medications amoxicillin, levofloxacin, cefotaxime, ceftriaxone, pefloxacin, cefepime, and ampicillin.

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Evaluation of Antibiotic Resistance and Biofilm Formation Ability Uropathogenic E. coli (UPEC) Isolated From Pregnant Women in Karaj

Cited by 6 publications

References 27 publications

Detection of Biofilm Formation and Antibiotic Susceptibility in Escherichia coli Isolated from the Urine of Pregnant Women at Mnazi Mmoja Hospital, Zanzibar, Tanzania

Detection of Biofilm Formation and Antibiotic Susceptibility in Escherichia coli Isolated from the Urine of Pregnant Women at Mnazi Mmoja Hospital, Zanzibar, Tanzania

Genetic Diversity, Carbapenem Resistance Genes, and Biofilm Formation in UPEC Isolated from Patients with Catheter-Associated Urinary Tract Infection in North of Iran

Molecular Analysis of Some Virulence Genes of Escherichia Coli Isolates from Wound and Burn Samples in Kirkuk City

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