Evaluation of antibacterial properties of organic gutta-percha solvents and synthetic solvents against Enterococcus faecalis

Maria, Rahul; Dutta, Smita D; Thete, Sanjay G; AlAttas, Mustafa Hussein

doi:10.4103/jispcd.jispcd_422_20

Cited by 10 publications

(3 citation statements)

References 27 publications

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“…Terpenoids make up the majority of and, in the opinion of Magria et al , have the power to kill bacteria by reducing membrane stability and increasing membrane permeability. [ 4 ] In a recent study, it was also discovered that the interactions between different volatile oil components had a synergistic impact that may also boost the antibacterial activity. [ 5 ] The leaves of the Myrtaceae plant species Eucalyptus globulus are used to make eucalyptus oil.…”

Section: Discussionmentioning

confidence: 99%

Determination of Antifungal Effect of Natural Oil and Synthetic Gutta Percha Solvents Against Candida Albicans: A Disc Diffusion Assay

Dutta

2023

Journal of Pharmacy and Bioallied Sciences

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Introduction: The practice of removing root canal fillings with solvent materials is frequently required to help an irrigation solution enter the tubules. The current research was aimed at assessing the antifungal properties specifically the candida albicans of the various solvent materials used for the gutta-percha (GP) material. Materials and Methods: Current research was aimed at as a lab method using the disk diffusion technique where the zone of inhibition (ZOI) was calculated. The materials that were analyzed were: orange oil, xylene, turpentine oil, chloroform, and eucalyptus oil. Candida albicans was the test organism employed in the investigation. The agar plates were covered with approximately 500 μL of the suspension. The sterile and empty disks were impregnated with 10 μL of pure GP solvents. These plates were incubated for one day at room temperature. The ZOI’s mean diameters were calculated for all five materials and quantified each solvent’s fungicidal activity. For intergroup comparison, ANOVA was utilized. P values < 0.05 were deemed substantial. Results: The maximum inhibition exhibited by the Eucalyptus Oil it was 19.01 ± 1.02 mm. This was followed by Xylene. The other three solvents Chloroform, Orange Oil, and Turpentine Oil exhibited a similar ZOI. When all the solvents were compared there was a significant variance of P < 0.001. However, there were significant variances for the Eucalyptus Oil and the Xylene to all the other solvents P < 0.001. Conclusion: This investigation showed that, in comparison to other solvents, the use of eucalyptus oil considerably reduced the levels of Candida Albicans.

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Section: Discussionmentioning

confidence: 99%

Determination of Antifungal Effect of Natural Oil and Synthetic Gutta Percha Solvents Against Candida Albicans: A Disc Diffusion Assay

Dutta

2023

Journal of Pharmacy and Bioallied Sciences

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“…OA12 was identified as Enterococcushirae. Although not a member of the Clostridiales order, the resistance of Enterococci to chemical treatment likely explains how this bacterium was retained after fractionation [46,47]. Similar to the other OA isolates, OA12 was enriched in Malaysian microbiomes [45] and was therefore included in subsequent experiments.…”

Section: Methodsmentioning

confidence: 99%

Functional characterization of helminth-associated Clostridiales reveals covariates of Treg differentiation

Sargsian,

Mondragón-Palomino,

Lejeune

et al. 2024

Microbiome

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Background Parasitic helminths influence the composition of the gut microbiome. However, the microbiomes of individuals living in helminth-endemic regions are understudied. The Orang Asli, an indigenous population in Malaysia with high burdens of the helminth Trichuris trichiura, display microbiotas enriched in Clostridiales, an order of spore-forming obligate anaerobes with immunogenic properties. We previously isolated novel Clostridiales that were enriched in these individuals and found that a subset promoted the Trichuris life cycle. In this study, we aimed to further characterize the functional properties of these bacteria. Results Clostridiales isolates were profiled for their ability to perform 57 enzymatic reactions and produce short-chain fatty acids (SCFAs) and hydrogen sulfide, revealing that these bacteria were capable of a range of activities associated with metabolism and host response. Consistent with this finding, monocolonization of mice with individual isolates identified bacteria that were potent inducers of regulatory T-cell (Treg) differentiation in the colon. Comparisons between variables revealed by these studies identified enzymatic properties correlated with Treg induction and Trichuris egg hatching. Conclusion We identified Clostridiales species that are sufficient to induce high levels of Tregs. We also identified a set of metabolic activities linked with Treg differentiation and Trichuris egg hatching mediated by these newly isolated bacteria. Altogether, this study provides functional insights into the microbiotas of individuals residing in a helminth-endemic region.

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“…GP removal is expedited by the use of solvents, such as orange extract, xylene, chloroform, and turpentine. 8 , 9 , 10 The effective dentine cutting ability of H-files make them suitable for retrieving separated instruments and GP from the RCS as part of NER. 11 , 12 …”

Section: Introductionmentioning

confidence: 99%

Effectiveness of H-files and Pro-Taper universal systems in removing Gutta-percha during endodontic retreatment: A comparative study

Atique,

Ali,

Haroon

et al. 2024

Journal of Taibah University Medical Sciences

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Evaluation of antibacterial properties of organic gutta-percha solvents and synthetic solvents against Enterococcus faecalis

Cited by 10 publications

References 27 publications

Determination of Antifungal Effect of Natural Oil and Synthetic Gutta Percha Solvents Against Candida Albicans: A Disc Diffusion Assay

Determination of Antifungal Effect of Natural Oil and Synthetic Gutta Percha Solvents Against Candida Albicans: A Disc Diffusion Assay

Functional characterization of helminth-associated Clostridiales reveals covariates of Treg differentiation

Effectiveness of H-files and Pro-Taper universal systems in removing Gutta-percha during endodontic retreatment: A comparative study

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