Evaluation of Antibacterial Effect of Silver Diamine Fluoride and Sodium Fluoride Varnish on Bacterial Biofilm (An In Vitro Study)

Abstract

Objectives:The present in vitro study was carried to compare the antibacterial effect of silver diamine fluoride (SDF 38%) and sodium fluoride varnish (NaF 5%) on inhibition of bacterial biofilm formation. subjects and methods: a total number of 48 biofilms were classified into three main groups (16 for each) and treated with. Group A: SDF 38% and Group B: NaF Varnish 5% for 1 minute only per day for three consecutive days. Group C: left untreated (control group). After treatment, fresh media was added to biofilms that were grown at 1st day and at 3rd day. Streptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus) were grown anaerobically. Then bacterial count was calculated by colony forming unit (CFU) per mL at 1 st and 3 rd day. Results: There was a statistically significant difference in the count of S. mutans and L. acidophilus between all groups as indicated by the One-way ANOVA test in which the highest bacterial count was found in Group C group followed by Group B while the least bacterial count was found in Group A. Thus, results recorded SDF 38 % showed a higher reduction than NaF 5% according to S. mutans and L acidophilus at 1 st and 3 rd day respectively. Conclusion: SDF is a non-invasive and simple approach for inhibiting S. mutans and L acidophilus growth that causing dental caries.