Evaluation of Antibacterial, Antioxidant, Anti-inflammatory and Anticancer Efficacy of Titanium-Doped Graphene Oxide Nanoparticles

Abstract

Introduction: The current development of nanoparticles (NPs) with significant antibacterial properties, low cost and low toxicity has made it possible to develop novel techniques for treatments in the medical field. The titanium metal oxide, when combined with a carbonaceous material like graphene, which has excellent absorbing capacity, is efficient in loading drugs and thus helps in drug delivery and also in biomedical applications like anticancer, anti-inflammatory, antioxidant, and antibacterial activities. Materials and methods: Titanium-doped graphene oxide nanoparticles (Ti/GO-NPs) were processed by the one-pot synthesis method; further characterization was performed by using UV-visible spectroscopy, Fourier transform-infrared spectroscopy (FT-IR), field emission electron microscopy (FE-SEM), and energy-dispersive X-ray spectroscopy (EDX) analysis and biomedical applications like anticancer, anti-inflammatory, antioxidant and antibacterial activities. Results: The synthesized end product of Ti/GO-NPs showed a creamy white appearance. Subsequent characterization studies of UV-Vis spectroscopy revealed a peak level of 373 nm at 24 hours and 404 nm after 48 hours. FT-IR analysis exhibited a broad absorption band within the range of 1000-3500 cm -1 , which was attributed to various chemical compounds of C-Br, C-I stretching, C=C bending, S=O stretching, O=H stretching, C=C stretching, H bonded and OH stretching to different absorbance wavelength ranges. SEM analysis exhibited quasi-spherical-shaped Ti/GO-NPs with an average particle size of 50- 100 nm and EDX analysis showed the elemental composition of 32.3% titanium 43.9% oxygen and 2.5% carbon. The antibacterial activity showed moderate activity against Staphylococcus aureus and no activity against Pseudomonas aeruginosa, Enterococcus faecalis and E. coli . The antioxidant activity exhibited 88% at 50 µg/mL concentration, the anti-inflammatory activity revealed 80% at 80 µg/mL concentration and the anticancer activity showed 21% at 150 µg/mL concentration. Conclusion: The characterization and biomedical application conclude that a combination of Ti/GO-NPs will be efficient in drug delivery. The study showed moderate antibacterial activity and significant antioxidant, anti-inflammatory and anticancer activities. Considering their physiochemical properties, absorption capacity and mechanism of drug delivery, Ti/GO-NPs can be incorporated into various applications in the medical field.