Evaluation of Antibacterial Activity and Antioxidant Potential of Different Extracts from the Leaves of Juniperus Phoenicea

Elmhdwi, Maraia F.; Attitalla, Idress Hamad; Khan, Barkat Ali

doi:10.4172/2157-7471.1000300

Cited by 12 publications

(17 citation statements)

References 29 publications

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“…This activity was highest at pure oil and was observed to decrease with concentration. This result was in accordance with previous studies which reported that bacterial strains were generally more sensitive to the essential oils than yeasts (Erkmen and Özcan, 2004;Helal et al, 2006;Obame et al, 2008).…”

Section: Resultssupporting

confidence: 93%

“…The essential oil of J. phoenicea showed both, bacteriostatic and bactericidal activity only against the four Gram-positive strains, with diameter zone inhibition ranging from 13.1 mm (E. faecium) to 15.8 mm (S. aureus), and MIC values ranging from 0.5 μL/mL (S. aureus) to 15μL/mL (L. monocytogenes EGD-e) and MBC values of 10 μL/mL (S. aureus and L. monocytogenes 4b), 15 μL/mL (L. monocytogenes EGD-e), and 30 μL/mL (E. faecium). In other study, Ramdani et al (2013) Elmhdwi et al (2015) reported that J. phoenicea extracts (70% methanol, 70% ethanol and 70% acetone) at concentration of 20%, 30% and 40% were effective against both Gram-positive (Staphylococcus aureus) and Gramnegative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Acinetobacter sp. ).…”

Section: Resultsmentioning

confidence: 97%

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Antimicrobial activity of essential oils of Juniperus phoenicea from North Western Algeria

2017

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Juniperus phoenicea (Family: Cupressaceae) is an evergreen tree widely distributed in North Africa including Algeria. The aim of this investigation was to analyse the antimicrobial potential of essential oils from J. phoenicea on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Streptococcus sp, Bacillus sp and Candida albicans using wells and discs diffusion methods. Broth dilution method was utilized to study the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The results showed a variable degree of antimicrobial activity. The diameters of inhibition zones for all test organisms were in the ranges of 7-21 mm, while MIC was from 62.5 to >500µl/ml and MBC from 250 to >500µl/ml. The highest antimicrobial activities were observed against Gram positive bacteria followed by Gram negative ones then Candida albicans. The findings provide the evidence that J. phoenicea as a good medicinal plant for further investigations.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 97%

Antimicrobial activity of essential oils of Juniperus phoenicea from North Western Algeria

2017

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“…This is in accord with similar reports for juniper species from other regions (Elmastaş et al, 2006;Moein et al, 2010;Emami et al, 2011;Höferl et al, 2014;Cioanca et al, 2015;Elmhdwi et al, 2015). However, antioxidant activity as reported in this study did not correlate with the total content of phenolics and flavonoids, except for extracts obtained from cuttings of small seedlings, which shared the highest antioxidant activity with that of C2-type callus and in vitro shoots.…”

Section: Secondary Metabolites and Antioxidant Activitysupporting

confidence: 61%

“…It could be that extraction with methanol was not the optimal choice. Elmhdwi et al (2015) showed that extraction of total phenols and flavonoids with 70% acetone in J. phoenicea gave better results than with 70% methanol or ethanol. They further elaborated that 70% acetone was more suitable for scavenging activity determination in J. phoenicea which resulted in higher reducing power.…”

Section: Secondary Metabolites and Antioxidant Activitymentioning

confidence: 99%

Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea

Al-Ramamneh¹,

Daradkeh²,

Rababah³

et al. 2017

Aust J Crop Sci

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Juniperus phoenicea is an ornamental shrub that is also used to flavor food and to supply medicines and timber. Its micropropagation is of industrial concern and can occur by axillary shoot multiplication. Microcuttings of J. phoenicia were established in vitro on Murashige and Skoog (MS) and Rugini Olive (OM) media in glass tubes (25 mm x 150 mm). Factors studied were explant length (0.5 or 1.5 cm) and orientation (horizontal or vertical), media strength (OM, ½OM, MS, ½MS) and the following growth regulators: the anti-auxin 2,3,5-triiodobenzoic acid (TIBA), the cytokinins 6-benzyladenine (BA) and thidiazuron (TDZ), and the growth retardant daminozide (DM). Microcuttings placed vertically on the surface of OM, ½OM and ½MS media without hormones exhibited axillary bud differentiation, but they were swollen and turned brown one month later when placed horizontally on the medium surface. The number of shoots, averaged across OM, ½OM and ½MS media, was significantly higher from longer (1.5 cm) than shorter (0.5 cm) microcuttings (4.11 versus 1.57 shoots microcutting -1 ) after 60 days of culture. TIBA or DM at 0.1 mg l -1 included in OM medium enhanced leaf differentiation, callus induction and formation of adventitious shoots over three months from 0.5 cm long microcuttings taken from in vitro shoots. The formation of adventitious shoots was sporadic and occurred at a rate of 1 shoot microcutting -1 in the presence of 0.1 mg l -1 DM. OM supplemented with 0.1 mg l -1 TIBA resulted in significantly the highest leaf differentiation (55 leaves microcutting -1 ), with a rooting rate of 40%. Contents of phenols, flavonoids and antioxidants were compared for cuttings from young seedlings, callus, in vitro shoots, and seeds. Antioxidant activity was significantly the highest for shoots grown on hormone-free OM medium and callus maintained on OM medium containing 0.1 mg l -1 2,4-D (94.7 and 94.3% inhibition of DPPH free radicals, respectively). Thus, different routes for in vitro regeneration of J. phoenicea can be of potential use for many biotechnological, pharmaceutical and food industry applications.

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“…The essential oils from this plant exhibited moderate antibacterial and antifungal activities. [5][6][7][8][10][11][12][13] Elmhdwi et al 14 demonstrated that the extracts from J. phoenicea had antibacterial activity against both Gram-positive and Gram-negative bacteria including multiple-drug-resistant (MDR) strains. This activity was attributed to the abundance of α-pinene and the overall chemical constituents of these extracts.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Libyan Juniperus phoenicea against Human Cancer Cell Lines A549, EJ138, Hepg2 and MCF7

Groshi¹,

Evans²,

Ismail³

et al. 2018

Pharm Sci

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Background: The current study was undertaken to assess the cytotoxicity of the leaves of Libyan Juniperus phoenicea (Cupressaceae) against human cancer cell lines. Methods: The cytotoxicity of the n-hexane, dichloromethane (DCM) and methanol (MeOH) extracts of the leaves of J. phoenicea (JP), obtained from sequential Soxhlet extractions, was assessed against four human cancer cell lines: EJ138 (human bladder carcinoma), HepG2 (human liver hepatocellular carcinoma), A549 (human lung carcinoma) and MCF7 (human breast adenocarcinoma) using the MTT assay. Results: The cell line A549 was the most sensitive to the JP extracts, with the highest level of cytotoxicity with the IC50 values of 16, 13 and 100 µg/mL for the DCM, n-hexane and MeOH extracts, respectively. However, generally the most potent cytotoxic extract across the other cells tested was the n-hexane extract, followed by the DCM extract, whilst the MeOH extracts showed little or no cytotoxicity. The percentage of viability of cells decreased as the concentration of test compounds increased. The cytotoxicity of various chromatographic fractions from the extracts was also studied against the A459 cells. For the n-hexane fractions, the IC50 values were 160, 62, 90, 30, 9.5 and 40 µg/mL for fractions 1 to 5 and 7, respectively. Fractions 4 and 5 showed the greatest effect. DCM fractions 2, 3 and 4 had the IC50 values of 60, 92 and 19 µg/mL, respectively, and DCM fractions 5 to 8 were non-cytotoxic. Fractions 1 and 2 of the MeOH extract were non-cytotoxic, whereas cytotoxicity was observed for fractions 3 and 4 with IC50 values of 50 and 85 µg/mL, respectively. Conclusion: The outcome of the present study suggested that the JP leaves possess cytotoxic activities. The high level of cytotoxicity of the n-hexane and DCM extracts suggested that lipophilicity might affect the cytotoxicity of JP, where the less polar compounds had the strongest cytotoxicity.

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Evaluation of Antibacterial Activity and Antioxidant Potential of Different Extracts from the Leaves of Juniperus Phoenicea

Cited by 12 publications

References 29 publications

Antimicrobial activity of essential oils of Juniperus phoenicea from North Western Algeria

Antimicrobial activity of essential oils of Juniperus phoenicea from North Western Algeria

Effects of explant, media and growth regulators on in vitro regeneration and antioxidant activity of Juniperus phoenicea

Cytotoxicity of Libyan Juniperus phoenicea against Human Cancer Cell Lines A549, EJ138, Hepg2 and MCF7

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