Evaluation of Antibacterial Activities of Tanzanian Moringa oleifera Extracts against Escherichia coli and Klebsiella pneumonia Clinical Isolates

Begum, Sartaz; Asenga, Jenipher S.; Ndesendo, Valence M. K.; Ngingo, Baraka L

doi:10.4314/tjs.v47i3.16

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…In addition Xiong et al, (2018) investigated the use of MOL as beds to disinfect the raw water from E. coli, which exhibited a high removal efficiency of 99.0 %, with a total coliform count of 3-4 cfu\ 100 ml. In the same line, Chandrashekar et al, (2020); Begum et al, (2021) indicated that a crude extract from Moringa contained a coagulant protein that exhibited cell aggregation and growth inhibition of E. coli and S. paratyphi B coliform bacteria, which was attributed to the presence of peptides with antibacterial properties in the Moringa extract. In a recent study conducted by Semanka et al, (2022), it was observed that reduction of coliform count recorded more than 65 % of the total coliform.…”

Section: Fecal-coliform Antibacterial Susceptibility To Molmentioning

confidence: 95%

Moringa oleifera leaf as a natural water purifier and causes decontamination of fecal-coliform bacteria

Abd-Elhalim

2022

Novel Research in Microbiology Journal

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This study aimed to focus on the Nile's raw water purification and sanitation in the Warraq al Hadar area, Egypt, using Moringa oleifera leaf powder. The water turbidity, pH, and total fecal-coliforms were assessed. The water turbidity and pH were decreased on addition of M. oleifera leaf powder at 2, 4, 6, 8, and 10 µg\ ml; with reductions of 49.8, 68.7, 87.4, 92.0, and 93.7 % for turbidity, while the pH was reduced by 12.37, 20.0, 28.14, 38.14, and 38.14%, respectively. Using the most probable number approach (MPN), the highest decrease in the coliform bacterial count was observed at 10 µg\ ml of MOL recording 60 cfu\ 100 ml. On estimating the in vitro inhibitory effect of M. oleifera leaf powder on the fecalcoliform bacterial strains using the agar well diffusion assay, Escherichia coli ATCC 8739 was the most affected strain, recording an inhibition zone diameter of 37.0 mm, while Salmonella typhi DSM 17058 was the least inhibited strain with an IZD of 30.0 mm. The minimum inhibitory concentration (MIC) of M. oleifera leaf powder was 6.25 µg\ ml for E. coli, Shigella sonnei DSM 5570, and S. typhi DSM 17058, while it was 12.5 µg\ ml for Enterococcus faecalis ATCC 7080. The minimum bactericidal concentration (MBC) of the M. oleifera leaf powder was equivalent to 25.0 µg\ ml for Enterococcus faecalis, 12.50 µg\ ml for S. typhi, and 6.25 µg/ ml for E. coli and Shigella sonnei. Biocompatibility of the M. oleifera leaf powder was confirmed using the oral epithelial cell lines (OEC), which recorded an IC 50 of 716.1 μg\ ml, whereas cell viability of 100.0, 94.1, 90.5, and 88.0 % was observed on application of the MOL powder at different doses of 25, 50, 100, and 150 μg\ ml, respectively.

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Section: Fecal-coliform Antibacterial Susceptibility To Molmentioning

confidence: 95%

Moringa oleifera leaf as a natural water purifier and causes decontamination of fecal-coliform bacteria

Abd-Elhalim

2022

Novel Research in Microbiology Journal

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Searching for new agents against Enterobacteriaceae from nature: approaches, potential plant species, isolated compounds, and their respective properties

Masota,

Zehe,

Vogg

et al. 2023

Phytochem Rev

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The rising trend of antibiotic-resistant infections around the world and the low antimicrobials development pipeline volume are necessitating continued efforts in the search for novel treatment options. The prominent success from fungi and bacteria as sources of antibiotics has long motivated widespread efforts in the search for antibacterial compounds from other natural sources including plants. This review aimed at appraising the approaches and outcomes from studies commissioned to evaluate the antibacterial activities of crude plant extracts and phytochemicals. Notably, the existing traditional practices provided the greatest motivation in screening for antibacterial properties of plants, whereby the need to validate ethnomedically reported potentials formed a crucial objective. Moreover, choices of experimental techniques to address different objectives were largely dependent on the prevailing access to resources, facilities, and technical skills. The lack of streamlined guidelines dedicated to testing of crude plant extracts have resulted into broad methodological variations and lack of a standardized classification system for antibacterial activities exhibited by plant extracts. Furthermore, libraries of 128 extracts from different plant species and 122 phytochemicals substantially active against the Escherichia coli and Klebsiella pneumoniae were assembled. This enabled the elucidation of existing patterns between the Minimum Inhibitory Concentrations (MICs) and studied plant families, plant tissues, extractants, phytochemical classes, as well as the rules of drug-likeness, penetration and accumulation. The insights provided in this review will potentially impart the ongoing efforts with improved experimental designs, inspire ideas for further studies and contribute to successful hunting for new antibacterial chemical scaffolds via in silico approaches. Graphical abstract

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Effects of Moringa oleifera seeds on the physicochemical properties and microbiological quality of borehole water from Botswana

Semanka

Seifu

Sekwati-Monang

2022

Journal of Water, Sanitation and Hygiene for Development

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This study was conducted to determine the effects of Moringa oleifera seeds on the physicochemical properties and microbiological quality of borehole water collected from Thamaga village in Botswana. Borehole water samples were subjected to two different Moringa seed treatment methods: M. oleifera crushed seed powder and Moringa seed extract. Five treatment levels (0, 10, 20, 30, 40 g or mL extract) were used for each treatment method. Borehole water treated with Moringa seed extract had significantly lower (p < 0.05) total dissolved solids and conductivity as compared with water treated with crushed Moringa seed powder. However, borehole water treated with Moringa seed extract had significantly higher (p < 0.05) pH and total suspended solids as compared with water treated with crushed Moringa seed powder. Escherichia coli and total coliforms were completely inhibited by Moringa seed extract after 2 h of treatment at a concentration level of 40 mL extract. It was observed that Moringa seed extract at a concentration level of 40 mL was the most effective in treating borehole water compared with Moringa crushed seed powder. In conclusion, Moringa seed extract can be used as an alternative and affordable source of borehole water treatment in rural Botswana.

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Evaluation of Antibacterial Activities of Tanzanian Moringa oleifera Extracts against Escherichia coli and Klebsiella pneumonia Clinical Isolates

Cited by 3 publications

References 14 publications

Moringa oleifera leaf as a natural water purifier and causes decontamination of fecal-coliform bacteria

Moringa oleifera leaf as a natural water purifier and causes decontamination of fecal-coliform bacteria

Searching for new agents against Enterobacteriaceae from nature: approaches, potential plant species, isolated compounds, and their respective properties

Effects of Moringa oleifera seeds on the physicochemical properties and microbiological quality of borehole water from Botswana

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