Evaluation of Anti-psoriatic Potential of the Fruit Rind of Punica granatum L.

Abstract

Background: Pomegranate (Punica granatum L.) is universally known for its therapeutic properties due to its potential bioactive compounds. However, there is no established scientific study on antipsoriatic activity of pomegranate fruit rind. The aim of the study is to evaluate the anti-psoriatic activity of the isolated compounds and the extract from the fruit rind of P. granatum. In our previous study, the isolated compounds were evaluated for antioxidant potential. In continuation to the previous investigation, the present study is taken up to evaluate the extract and compounds for in vitro anti-psoriatic activity. Methods: Chromatographic techniques were employed to isolate the compounds from the aqueous acetone extract and in vitro anti-psoriatic activity was determined by thymidine phosphorylase inhibition assay. Results: From previous phytochemical investigation, three compounds were identified as Punicalagin, 2,3(S)-hexahydroxydiphenoyl-D-glucose and Punicalin. In the present study, the extract and the compounds were evaluated for anti-psoriatic activity. The results reveal that the isolated three compounds showed inhibitory activity of 89% to 95% against thymidine phosphorylase. Aqueous acetone extract also exhibited 87% inhibition. Conclusion: Punica granatum is an ideal plant for further investigation to prove its anti-psoriatic activity.

Recent Updates on Mechanisms of Resistance to 5-Fluorouracil and Reversal Strategies in Colon Cancer Treatment

“…The condition is due to the loss of function of TYMP gene following homozygous mutations c.1283 G > A, and c.1284 T > A, as well as mutations in DNA polymerase subunit gamma (POLG) and ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B) genes that lead to TYMP deficiency [ 252 , 253 , 254 ]. Interestingly, increased TYMP expression has been reported in cases of local inflammations such as rheumatoid arthritis and psoriasis, suggesting its plausible causal link with stress conditions such as hypoxia in the tumor microenvironment [ 255 , 256 ]. Indeed, TYMP overexpression may promote adaptive responses mediated by the hypoxia-inducible factor (HIF-1) pathway that prevents hypoxia-induced apoptosis that is triggered upon continuous chemotherapy [ 229 , 242 , 257 ].…”

Pomegranate peel polyphenols as an antioxidant additive for the development and characterization of a new active pectin edible film

“…Sun, Tao, et al (2017) reported DPPH radical inhibition values close to 90% at a concentration of 40 ppm using three separate purified compounds (ellagic acid, punicalin, and punicalagin). On the other hand, Jacob et al (2018) used the ABTS method to assess the antioxidant potential of two distilled pomegranate peel compounds (punicalagin and punicalin) at a concentration of 200 ppm, with a 100% inhibition percentage. Aguilar‐Zárate et al (2017) tested the antioxidant activity of distilled punicalagin using two methodologies (DPPH and ABTS), reporting a 50% inhibitory concentration with concentrations of 109 and 151 ppm, respectively.…”

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“…Natural products have proven to be alternative and potential sources of synthetic drugs. Studies have shown that crude extracts or purifed chemical constituents of various medicinal plants are often more efective than synthetic antioxidants [6]. P. granatum (pomegranate) has been widely used in the food and pharmaceutical industries for a variety of applications and as a source of antioxidants to combat autoxidation-induced pathologies [7,8].…”

“…In addition to its nutritional benefts, the fruit is used empirically for the treatment of various diseases, such as acidosis, dysentery, microbial infections, parasitic infections, hemorrhage, and respiratory pathologies [9,11]. Te pharmacological properties of pomegranate have a long history, and recent decades have seen a growing interest in its therapeutic efects [6].…”