Evaluation of anti-osteoarthritic activity of Vigna mungo in papain induced osteoarthritis model

Abstract

Aim:This study was carried out to evaluate the effect of Vigna mungo hydroalcoholic extract (VMHA) by papain induced osteoarthritis (OA) in the rat model.Materials and Methods:OA was induced by intra-articular injection of papain (4% w/v) along with cysteine (0.03 M) on day 1, 4 and 7 in rats and VMHA was administered orally in three doses (100, 200 and 400 mg/kg) after last papain injection. The anti-osteoarthritic activity was evaluated by measuring knee joint diameter, grip strength, locomotion activity and hanging time. Histopathological analysis and acute toxicity study were also performed.Results:VMHA improved inflammatory condition with all the doses, but significant (P < 0.05) attenuation of inflammation was present only with 400 mg/kg dose. The grip strength, locomotion activity and hanging time were also significantly (P < 0.05) improved at dose level of 100 mg/kg however other two doses (200 mg/kg and 400 mg/kg) were not found to be effective. VMHA did not show any mortality or any toxic clinical signs after oral administration of 2 g/kg dose.Conclusion:VMHA improved arthritic condition by significantly reducing pain and inflammation.

An impaired healing model of osteochondral defect in papain-induced arthritis

“…Along with these inflammatory cytokines, MMP levels and free radical products increase [ 15 ]. All of these factors induced by intra-articular injection of papain lead to arthritis symptoms in rabbits, which mimic the conditions found in human OA [ 16 ]. In this study, an OA-OCD rabbit model was established by intra-articular injection of papain followed by the creation of a cylindrical osteochondral defect in the femoral patellar groove.…”

Medicinal Properties of Māsh (Vigna mungo (Linn.) Hepper): A Comprehensive Review

“…Patel et al, (2015) reported that Vigna mungo hydroalcoholic extract (VMHA) improved arthritic condition significantly by reducing pain and inflammation. Improvement in pain behavior could result from the inhibition of prostaglandins by flavonoids present in VMHA and/or maybe through central pathways of analgesia 32 .…”

“…Vigna mungo has been reported pharmacologically to possess anti-inflammatory activity 32 . Anti-inflammatory activity is due to ethanol extracts mainly polyphenols 16 .…”

“…Used in rheumatism, paralysis, aching bones, affections of the nervous system, and diseases of the liver 1,2,5,11,14,16,32 . It is medicinally used both internally and externally, internally used in the form of decoction in dyspepsia, strangury, constipation, hepatopathy, neuropathy, and agalactia.…”

The effect and mechanism of QufengZhitong capsule for the treatment of osteoarthritis in a rat model

“…Then the rats were induced to grasp rigid bars attached to a digital force gauge (Aikoh Engineering Corporation, Osaka, Japan) with their right hind limb. Each rat was gently pulled backward, and the tension reading of the digital force gauge was defined as the grip strength [ 21 ]. The test was performed three consecutive times, and the mean value of the three tests was recorded as the grip strength.…”