Evaluation of anti-diabetic potential of leaves and stem of Flacourtia jangomas in streptozotocin-induced diabetic rats

Abstract

Objectives:To study the efficacy of combination of Flacourtia jangomas leaf and stem (1:1) methanolic extract (MEFJ) in streptozotocin (STZ)-induced diabetic rats and to investigate the qualitative phytochemical present in the extract. The study also aims to evaluate acute and short-term general toxicity of the extract in rats.Material and Methods:MEFJ of leaves and stem was subjected to preliminary qualitative phytochemical investigations by using standard procedures. The extract (400 mg/kg p.o.) was screened for antidiabetic activity in STZ-induced diabetic rats (30 mg/kg, i.p.). Acute oral toxicity study for the test extract of the plant was carried out using OECD/OCED guideline 425.Results:Phytochemical analysis of MEFJ of leaves and stem revealed the presence of flavonoids, saponins, carbohydrates, steroids, tannins, and phenolic compounds. In acute toxicity study, no toxic symptoms were observed for MEFJ up to dose 2000 mg/kg. Oral administration of MEFJ for 21 days exhibited highly significant (P < 0.01) hypoglycemic activity and also correction of altered biochemical parameters, namely cholesterol and triglycerides significantly (P < 0.05). Urine analysis on 1st day showed the presence of glucose and traces of ketone in the entire group except normal control group. However, on 21st day glucose and ketone traces were absent in MEFJ- and glibenclamide-treated groups while they were present in diabetic control. The data were analyzed using analysis of variance followed by Dunnett’s test.Conclusion:The observations confirm that methanolic extract of the leaf and stem of the plant has antidiabetic activity and is also involved in correction of altered biological parameters. It also warrants further investigation to isolate and identify the hypoglycemic principles in this plant so as to elucidate their mode of action.

Iron Reducing and Radical Scavenging Activities of 13 Medicinal Plants From Côte d’Ivoire

“…B. abyssinica is traditionally used for the treatment of malaria, 21 cancer, ulcer, rheumatism, wounds 22 and diabetes. 23 Free radicals are involved in the genesis of most of these diseases. Interestingly, the plant showed high activity in the current study.…”

Flacourtia jangomas

“…Methanolic extract of the leaf and stem of Flacourtia jangomas was found to have hypoglycaemic activity (Singh and Singh 2010 ) . Oral administration of the extract to streptozotocin (STZ)-induced diabetic rats for 21 days exhibited highly signi fi cant hypoglycemic activity and also signi fi cantly restored altered biochemical parameters, namely cholesterol and triglycerides.…”

Evaluation of Antihyperglycemic and Antihyperlipidemic Activity of Leaf Extracts of Breynia vitis-idaea in Alloxan Induced Diabetic Rats

“…In the present scenario, the demand for herbal products is growing expo nentially throughout the world and major pharmaceutical companies are currently conducting extensive research on plant material for their potential medicinal value. 5 Many medicinal plants used in ethnomedical practices in India are known or little known to scientific world. The main objective of the study was to assess the hypoglycemic and hypolipidemic potential of leaves of Coral berry tree Breynia vitis-idaea belonging to the family Euphor biaceae.…”