Evaluation of anti-diabetic activity of glycyrrhizin-loaded nanoparticles in nicotinamide-streptozotocin-induced diabetic rats

Rani, Ruma; Dahiya, Shakti; Dhingra, Dinesh; Dilbaghi, Neeraj; Kim, Ki‐Hyun; Kumar, Sandeep

doi:10.1016/j.ejps.2017.05.068

Cited by 89 publications

(46 citation statements)

References 41 publications

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“…In vitro release studies were accomplished for optimized batch MN3 and pure metformin by dialysis sac method [6]. Accurately weighed samples were placed in dialysis bags (12-14 kDa cut-off, HiMedia, India) and tied with dialysis thread.…”

Section: In Vitro Drug Release Studiesmentioning

confidence: 99%

“…Metformin improves the action of insulin at the cellular level without affecting its secretion [5]. It has low bioavailability (40-60%) and short biological half-life (1.0-4.5 h) necessitate repeated administrations of doses (1.5-2.0 g/day) in two or three daily dose regiments to retain effective plasma concentrations [6][7][8][9]. The diabetic patients require life-long treatment and repeated administrations of doses reduce patient compliance and augment the frequency of side effects (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Nanoparticulate pharmaceutical formulations have properties of improved bioavailability, controlled, and prolonged drug release time with efficacy, safety and predicted therapeutic effect [5,16]. Various natural, synthetic, and semi-synthetic polymers have been investigated as a drug carrier to utilize the benefits of nanotechnology in formulations [6,[16][17][18][19][20][21]. There is a persistent effort among the researchers to improve the formulation of metformin to attain optimal therapeutic response.…”

Section: Introductionmentioning

confidence: 99%

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Preparation and Characterization of Metformin Loaded Stearic Acid Coupled F127 Nanoparticles

Kamboj

Verma

2018

Asian J Pharm Clin Res

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Objective: The objective of this study was to prepare and evaluate metformin nanoparticles (MN) using stearic acid-coupled F127 (SAF127) copolymer and polyvinyl alcohol by emulsion solvent evaporation technique.Method: Metformin is the first-line drug for the treatment of type II diabetes mellitus belongs to Biopharmaceutical Classification System Class III. The prepared MN was characterized for particle size, polydispersity index (PDI), zeta potential, drug entrapment, percentage yield, in vitro drug release, and stability studies. The compatibility studies were performed by Fourier transform infrared (FTIR) and differential scanning calorimetry (DSC). The crystallographic and surface properties were studied by X-ray diffractometry and scanning electron microscopy, respectively.Results: The mean particle diameter of prepared nanoparticles ranged from 207.8 to 977.64 nm, PDI value ranged from 0.146 to 0.694, and zeta potential ranged from −20.5 to −6.97 mV. The drug entrapment efficiency of these nanoparticles varies between 18.81 to 69.01 %. The drug to SAF127 copolymer (10/30 w/w) ratio (MN3) showed optimum results. The MN3 had spherical morphology with semi-amorphous nature. The results of FTIR and DSC analysis showed that there was no significant interaction between drug and excipients. The prepared polymeric nanoparticles were stable at 5±3°C up to 3 months. In vitro release of drug from MN3 was 20.52% in the first 1 h and remaining drug was released up to 30 h.Conclusion: The results of this study confirmed the sustained drug release profile of metformin loaded SAF127 copolymer nanoparticles. These nanoparticles can be best stored up to 3 months.

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Section: In Vitro Drug Release Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preparation and Characterization of Metformin Loaded Stearic Acid Coupled F127 Nanoparticles

Kamboj

Verma

2018

Asian J Pharm Clin Res

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“…In vitro dissolution studies were performed for optimized GN1 batch and pure glipizide by modified dialysis sac method 18…”

Section: In Vitro Dissolution Studiesmentioning

confidence: 99%

Palmitic Acid-Pluronic F127-Palmitic Acid Penta-Block Copolymer as a Novel Nanocarrier for Oral Delivery of Glipizide

Kamboj¹,

Verma²

2018

tjps

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Objective:The aim of the present study is to develop nanotechnology-based oral formulations of Glipizide to enhance bioavailability and to eliminate the frequent oral administration of the conventional dosage form. Glipizide is an antidiabetic drug with short biological half-life with limited oral bioavailability. Noval Palmitic acid-Pluronic F127-Palmitic acid (PAF127) pentablock copolymer based prolonged release glipizide nanoparticles (GN) were prepared and screened for in vitro and in vivo studies.Methods: GN was prepared using novel PA-F127 pentablock copolymer by solvent evaporation technique. The prepared nanoparticles were evaluated for particle size, polydispersity index (PDI), zeta potential, entrapment efficiency, percentage yield, drug excipient compatibility using FTIR and DSC analysis, XRD, SEM, In vitro drug release studies, stability studies and in vivo pharmacokinetic studies. Results:The results of FTIR and DSC analysis revealed the absence of drug-excipient interactions. The optimized GN1 has particle size 242.60 ± 4.20 nm, PDI 0.171 ± 0.014 and zeta potential -21.41 ± 0.462 mV. Prepared nanoparticles were spherical in shape and showed semi-amorphous characteristics. In vitro release studies showed 34.43 ± 4.8 % drug was released in first 8 h, 56.11 ± 4.12 % glipizide were released further for 24 h. The GN1 was found to be stable at 5 ± 3 ºC up to three months. Pharmacokinetic studies showed that the orally administered GN1 were superior with C max 2.35 fold, t max 1.6 fold, area under the curve (AUC 0→∞ ) 3.3 fold and mean residence time (MRT) 1.2 fold as compared to pure glipizide (p < 0.05). Conclusion:The study concluded that the bioavailability of newly developed GN1 was successfully prolonged and frequent oral administration problem with conventional dosage form can be defeated for diabetes treatment.

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“…Glycyrrhizae Radix et Rhizoma (GRER) has been used as a medicinal plant and dietary supplements since ancient times in China, Japan, Italy, United States, and other different countries and regions [1][2][3][4]. Modern research studies showed that GRER had extensive pharmacological effects in antispasmodic, antidiabetic, antiosteoporosis, antidepressive, antitussive and expectorant, hepatoprotective, and memory-enhancing [5][6][7][8][9][10][11]. e major bioactive components of GRER were saponins, flavonoid glycosides, and free phenolic compounds [12].…”

Section: Introductionmentioning

confidence: 99%

Glycyrrhizae Radix et Rhizoma Processed by Sulfur Fumigation Damaged the Chemical Profile Accompanied by Immunosuppression and Liver Injury

Jiang

Xiao

Yan³

et al. 2020

BioMed Research International

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Glycyrrhizae Radix et Rhizoma (GRER) has been used as a medicinal plant and dietary supplements for its beneficial effect in immunomodulatory effects. Sulfur fumigation (SF) processing was widely used in the storage and maintenance of Chinese medicine because of its convenience and cheapness. However, the disadvantage of SF has been reported, but the systematic study of SF on GRER was deficient. In this paper, the active ingredients, sulfur-fumigated products, immunomodulatory effect, and liver injury of SF-GRER were studied. After SF, the liquiritin decreased from 4.49 ± 0.03 mg/g to 3.94 ± 0.08 mg/g (P<0.01). Compared with the NSF-GRER group, the SF-GRER group showed a decreased immunoregulation in the thymus index, spleen index, and serum IL-6 and SOD levels (P<0.05). After 2 weeks of continuous intragastric administration of SF-GRER in healthy mice, the level of serum aspartate aminotransferase (AST) significantly increased (P<0.05) and the area of liver lesion significantly increased compared with the NSF-GRER (P<0.05) group. The sulfonated products (m/z, 631.13) corresponding to liquiritin apioside (m/z, 551.17) and isoliquiritin apioside (m/z, 551.17) were screened out in SF-GRER by using UPLC-Orbitrap-MS. The sulfonated products provided in this paper were discovered for the first time and could be powerfully applied for the identification of SF-GRER. SF destroyed the chemical composition of GRER, inhibited immunoregulation, and induced liver injury. The feasibility of this processing method needs to be reconsidered.

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Evaluation of anti-diabetic activity of glycyrrhizin-loaded nanoparticles in nicotinamide-streptozotocin-induced diabetic rats

Cited by 89 publications

References 41 publications

Preparation and Characterization of Metformin Loaded Stearic Acid Coupled F127 Nanoparticles

Preparation and Characterization of Metformin Loaded Stearic Acid Coupled F127 Nanoparticles

Palmitic Acid-Pluronic F127-Palmitic Acid Penta-Block Copolymer as a Novel Nanocarrier for Oral Delivery of Glipizide

Glycyrrhizae Radix et Rhizoma Processed by Sulfur Fumigation Damaged the Chemical Profile Accompanied by Immunosuppression and Liver Injury

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