Evaluation of anti-diabetic activity of different extracts of Corchorus trilocularis in STZ induced diabetes in rats

Abstract

Aim- The main aim of the present study is to evaluate the anti-diabetic study of different extracts of Corchorus trilocularis in STZ induced diabetes in rats. Material and Methods- Powdered drug 500 gm was weighed and packed in soxhlet. The drug was continuously extracted with petroleum ether for about 72 hours. Complete defatting was ensured by placing a drop form the thimble on a filter paper give any oily spot. The mark was dried in air to remove traces of petroleum ether. Defatted mark was subjected to extraction with ethyl acetate in soxhlet apparatus and then again extracted by using ethanol, butanol and finally by using water. Acute oral toxicity test was carried out according to the OECD guideline No. 423. After fasting 18 hours, the rats were injected intraperitoneal injection through tail vein with a single dose of 40 mg/kg Streptozocin, freshly dissolved in citrate buffer (pH 4.5). Then serum samples were also used to analyze for serum Total Cholesterol (TC), Triglycerides (TG), High Density Lipoprotein Cholesterol (HDL-C), Low Density Lipoprotein Cholesterol (LDL-C) and Very Low Density Lipoprotein Cholesterol (VLDL-C) and atherogenic index (AI). Results- Petroleum ether, ethanolic and butanolic extracts had moderately significant effects (p<0.01) on 14th and 21st days. However, aqueous extracts showed significant effect (p<0.05) in glucose levels. The effect of aqueous extract is very less as compared to other extracts. Conclusion- The present investigation comprises of the phytochemical and pharmacological investigations of leaves of Corchorus trilocularis for the antidiabetic activity. Keywords- Anti-diabetic, Different extracts, Corchorus trilocularis, STZ induced diabetes, (HDL-C), Low Density Lipoprotein Cholesterol (LDL-C), Very Low Density Lipoprotein Cholesterol (VLDL-C)