Evaluation of Antagonistic Activities of Bacillus Spp. against Certain Bacteria of Medical Importance

Gupta, Kartikey Kumar; Rana, Deepanshu

doi:10.26832/24566632.2017.020419

Cited by 3 publications

(5 citation statements)

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“…: Antmicrobial activity of the isolated Enterobacter sp. designated as KD111 was evaluated using cross-streak method (Gupta and Rana, 2017) Enterobacter sp. KD111 was streaked onto NAM plates as a single streak in the centre and incubated at 37°C for 24h.…”

Section: Methodsmentioning

confidence: 99%

“…Secondary metabolites from many microorganisms have been successfully developed and produced up to commercial level in the form of antibiotics such as Gramicidin and Rifampin being produced by Bacillus and Streptomyces spp. respectively (Waites et al, 2008;Awais et al, 2010;Mahajan and Balachandran, 2012;Gupta and Rana, 2017). use of antibiotics that pose serious health implications on patient care in terms of increasing substantial morbidity and mortality (Maataoui et al, 2014;Balachandran et al, 2015;Gay et al, 2017;Ekwanzala et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Preliminary study on inhibitory activity of Enterobacter sp. strain KD111 isolated from the Cow feces

Gupta

Rana

2018

Environ. Conserv. J.

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The objective of the present study was to isolate Enterobacter sp. from the faecal matter of cow followed by its screening for antimicrobial activity against Gram-positive and Gram-negative bacteria having clinical importance. On the basis of morphological and biochemical characterisation of seven isolates obtained from cow dung sample, isolate KD111 was probably identified as Enterobacter sp. and screened for its inhibitory activity against 14 test organisms comprising both Gram-positive and Gram-negative types using cross-streak method, in which both i.e. test organisms and isolates were streaked perpendicularly on nutrient agar plates followed by measurement of inhibition zone between the streaks after incubation. The preliminary screening revealed significant antimicrobial activity of Enterobacter sp. against Salmonella typhi (MTCC 3216), Escherichia coli, Staphylococcus aureus (MTCC 7443) and Bacillus cereus (MTCC 6728) with highest inhibition against Salmonella typhi (MTCC 3216) and Bacillus cereus (MTCC 6728). Our results indicate that Enterobacter sp. may act as a producer of bioactive antimicrobial metabolites and therefore should be analysed further for its possible application as therapeutic agent.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preliminary study on inhibitory activity of Enterobacter sp. strain KD111 isolated from the Cow feces

Gupta

Rana

2018

Environ. Conserv. J.

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“…108 Bacilli are capable of degrading organic materials, ammonia, sulfides, and more, 109 and some are capable of producing surfactants which can disrupt a variety of biological and synthetic oils. 106,111,112 They may also compete with other common environmental bacteria and fail to persist on a surface, 113 potentially requiring continuous or frequent application. 106,111,112 They may also compete with other common environmental bacteria and fail to persist on a surface, 113 potentially requiring continuous or frequent application.…”

Section: Pro -Microb Ial Cle Aningmentioning

confidence: 99%

“…Similarly, incorporating microorganisms into systems to degrade materials has been effectively used in water systems, including aquariums, wastewater treatment plants, bioswales, green infrastructure, and more. 102 More recently, alternatives to traditional household cleaners and disinfectants have become commercially available that Bacilli are well-known for their ability to survive adverse environmental conditions, form endospores, and produce antimicrobial compounds 104,105 and have also been demonstrated to antagonize a handful of bacterial pathogens, 106 even on non-porous surfaces. 107 Moreover, Bacillus subtilis poses little threat to individuals who are not immunocompromised and is commonly used as a dietary probiotic.…”

Section: Pro -Microb Ial Cle Aningmentioning

confidence: 99%

“…110 Yet, bacilli are commonly found in all environments and may compete with each other, possibly precluding the ability of the probiotic cleaner species to form a biofilm. 106,111,112 They may also compete with other common environmental bacteria and fail to persist on a surface, 113 potentially requiring continuous or frequent application.…”

Section: Pro -Microb Ial Cle Aningmentioning

confidence: 99%

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From one species to another: A review on the interaction between chemistry and microbiology in relation to cleaning in the built environment

et al. 2019

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Since the advent of soap, personal hygiene practices have revolved around removal, sterilization, and disinfection—both of visible soil and microscopic organisms—for a myriad of cultural, aesthetic, or health‐related reasons. Cleaning methods and products vary widely in their recommended use, effectiveness, risk to users or building occupants, environmental sustainability, and ecological impact. Advancements in science and technology have facilitated in‐depth analyses of the indoor microbiome, and studies in this field suggest that the traditional “scorched‐earth cleaning” mentality—that surfaces must be completely sterilized and prevent microbial establishment—may contribute to long‐term human health consequences. Moreover, the materials, products, activities, and microbial communities indoors all contribute to, or remove, chemical species to the indoor environment. This review examines the effects of cleaning with respect to the interaction of chemistry, indoor microbiology, and human health.

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Drug-Resistant Proteus Virulence Factors Characterization and Their Inhibition Using Probiotic Bacteria

Zaman

Ullah

Qasim

et al. 2022

Jundishapur J Microbiol

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Background: The genus Proteus is a Gram-negative bacterium with a unique characteristic of swarming. Mainly three species are involved in initiating urinary tract infections in the community and in immunocompromised patients, particularly in patients going through long-term catheterization. Due to their strong virulence factors like biofilm formations, protease, and hemolysin, they can lead to lengthening infections in affected individuals. Probiotics are live bacteria and yeasts that are beneficial to human health and can be used as an alternative for the control of nosocomial diseases. Lactobacilli are one of the common probiotics mostly found in yogurt and other fermented foods that have been used as a substitute for infection control. Objectives: The current study was designed to screen potential probiotic bacteria to encounter antibiotic-resistant and virulent Proteus species. Methods: In the current study, using probiotics, already known antibiotic-resistant isolates (n = 25) of Proteus were processed to characterize their virulence factors and their inhibition. Biofilm formation, protease, and hemolysin activities were studied using different phenotypic detection methods. Further, their virulence genes zapA, flg, hmpA, mrp, and rsbA were explored using their genomic DNA. These isolates were found resistant to different classes of antibiotics, and a strategy was designed to inhibit their growth by using probiotic bacteria isolated from the soil. Results: Virulence factors first, all isolates were subjected to biofilm detection, and they were 32% (n = 8) strong, 40% (n = 10) moderate, 16% (n = 4) weak, and 12% (n = 3) non-biofilm producers. All isolates were positive for swarming activity by showing a differentiated ring form of growth. Protease activity showed 56% (n = 14) isolates. Only 24% (n = 6) of isolates were positive for hemolysin. Virulence factors and molecular mechanisms were studied, and gene rsbA responsible for swarming was amplified in 17 (68%) Proteus isolates, and mrp responsible for fimbria was detected in 19 (76%) bacterial isolates. Further, these isolates were subjected to flagella, protease, and hemolysin, and it was revealed that flg 11 (44%), 13 (52%) protease coding zapA, and hmA gene coding hemolysin were amplified in 2 (8%) Proteus isolates. Probiotic bacteria isolated from soil samples were probed for antagonistic activity against Proteus species. The probiotic bacteria were identified as Lactobacillus plantarum, Bacillus subtilis, and B. licheniformis. Due to their strong growth inhibitory effects against Proteus, it is crucial to characterize further the metabolites that have shown suppressive results against Proteus. Conclusions: Findings from the current study will provide new avenues for drug development and also help clinicians manage resistant pathogens in healthcare settings. Probiotic applications for infection control can be useful in treating resistant pathogens. Further purification and characterization of metabolites will provide alternative options for managing resistance issues in microbes.

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Evaluation of Antagonistic Activities of Bacillus Spp. against Certain Bacteria of Medical Importance

Abstract: Collection of sample:Dung sample of Desi cow breed was collected aseptically from cow shed located in Saharanpur, Uttar Pradesh. The sample was analysed immediately after transporting to the laboratory (Gupta and Rana, 2016a).

Cited by 3 publications

References 20 publications

Preliminary study on inhibitory activity of Enterobacter sp. strain KD111 isolated from the Cow feces

Preliminary study on inhibitory activity of Enterobacter sp. strain KD111 isolated from the Cow feces

From one species to another: A review on the interaction between chemistry and microbiology in relation to cleaning in the built environment

Drug-Resistant Proteus Virulence Factors Characterization and Their Inhibition Using Probiotic Bacteria

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