Evaluation of anion-exchange liquid chromatography for purification of hemoglobin from peptides and other proteins.

Simoni, Jan; Simoni, Grazyna; Feola, Mario; Canizaro, Peter C.

doi:10.1016/0003-2670(91)87020-8

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Hemoglobin purification has since been improved using high-speed centrifugation, ultrafiltration, solvent extraction, dialysis, crystallization, gel permeation chromatography, anion and cation-exchange chromatography, affinity chromatography, and combinations of such techniques. ,,,− Purity was assessed using gel electrophoresis, isoelectric focusing, thin-layer chromatography, and high-pressure liquid chromatography. However, most papers provide little quantitative information on Hb purity and many of the preparations used appear to have still contained other proteins and peptides, lipids, and metHb. ,, As for all proteins, Hb is heat sensitive. It is denaturated when temperature exceeds about 55 °C .…”

Section: Hemoglobin Purificationmentioning

confidence: 99%

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Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

2001

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Pharmaceutical Corporation in San Diego; however, the opinion expressed here are solely his and not necessarily those of the Corporation. Jean G. Riess received his chemical engineer and doctorat-e `s-sciences degrees (the latter with Professor Guy Ourisson in 1963) from the University of Strasbourg. He then spent two years with John Van Wazer at Monsanto in Saint-Louis, MO, learning some phosphorus and transitionmetal chemistry. In 1968 he became Professor at the University of Nice, France, where he founded, directed, and eventually became honorary director of the Unite ´de Chimie Mole ´culaire (associated with the Centre National de la Recherche Scientifique). His research successively involved phosphorus chemistry, transition-metal chemistry, organometallics, and eventually perfluorochemicals. His present interests are in fluorocarbons, fluorinated amphiphiles, and their colloid chemistry, including fluorocarbon emulsions for in vivo O 2 delivery, fluorinated self-assemblies, and drug delivery systems. Professor Riess has published about 360 papers and holds ca. 25 patents. He has served on numerous councils and committees and has chaired or co-chaired international conferences on phosphorus chemistry and blood substitutes. He has won awards from the French Academy of Sciences, French Chemical Society, Alexander von Humboldt Stifftung, City of Nice and Controlled Release Society, as well as Alliance's first Distinguished Contribution Award. He holds an honorary Research Associate position at the University of California at San Diego and sits on the Board of Directors of Alliance Pharmaceutical Corp.

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Section: Hemoglobin Purificationmentioning

confidence: 99%

“…However, most papers provide little quantitative information on Hb purity and many of the preparations used appear to have still contained other proteins and peptides, lipids, and metHb. 178,560,561 As for all proteins, Hb is heat sensitive. It is denaturated when temperature exceeds about 55 °C.…”

Section: Hemoglobin Purificationmentioning

confidence: 99%

Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

2001

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“…Ultra-purification of hemoglobin has been performed by several groups [ 20 , 21 , 22 , 23 ]. Hemoglobin was isolated from the blood through hemolysis of RBCs, a method reported by Russo and Sorstokke (1973) [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

Red Blood Cell Substitutes: Liposome Encapsulated Hemoglobin and Magnetite Nanoparticle Conjugates as Oxygen Carriers

Hafeez

Zaidi²

2023

IJMS

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The established blood donation and transfusion system has contributed a lot to human health and welfare, but for this system to function properly, it requires a sufficient number of healthy donors, which is not always possible. Pakistan was a country hit hardest by COVID-19 which additionally reduced the blood donation rates. In order to address such challenges, the present study focused on the development of RBC substitutes that can be transfused to all blood types. This paper reports the development and characterization of RBC substitutes by combining the strategies of conjugated and encapsulated hemoglobin where magnetite nanoparticles would act as the carrier of hemoglobin, and liposomes would separate internal and external environments. The interactions of hemoglobin variants with bare magnetite nanoparticles were studied through molecular docking studies. Moreover, nanoparticles were synthesized, and hemoglobin was purified from blood. These components were then used to make conjugates, and it was observed that only the hemoglobin HbA1 variant was making protein corona. These conjugates were then encapsulated in liposomes to make negatively charged RBC substitutes with a size range of 1–2 μm. Results suggest that these RBC substitutes work potentially in a similar way as natural RBCs work and can be used in the time of emergency.

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Chromatographic analysis of biopolymers distribution in “poly-hemoglobin”, an intermolecularly crosslinked hemoglobin solution

Simoni

Feola

1993

Analytica Chimica Acta

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Evaluation of anion-exchange liquid chromatography for purification of hemoglobin from peptides and other proteins.

Cited by 11 publications

References 10 publications

Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

Red Blood Cell Substitutes: Liposome Encapsulated Hemoglobin and Magnetite Nanoparticle Conjugates as Oxygen Carriers

Chromatographic analysis of biopolymers distribution in “poly-hemoglobin”, an intermolecularly crosslinked hemoglobin solution

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