Evaluation of animal models for predicting skin penetration in man*1

Reifenrath, William G.; Chellquist, Eric M.; Shipwash, Edward A.; Jederberg, Warren W.

doi:10.1016/0272-0590(84)90156-8

Cited by 66 publications

(4 citation statements)

References 6 publications

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“…Porcine skin is an excellent model for studying penetration of human skin because its thickness and absorption rates are comparable to those of human skin (Bronaugh et al 1982; Monteiro-Riviere and Riviere 1996; Reifenrath et al 1984). In the present study, we were surprised that after 14 consecutive days of topical dosing, the Ag-nps did not cause any macroscopic irritation, although the gray appearance of the skin due to the deposition of Ag-nps may have masked any subtle signs of erythema.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro

Samberg

Oldenburg

Monteiro‐Riviere

2010

Environ Health Perspect

450

263

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IntroductionProducts using the antimicrobial properties of silver nanoparticles (Ag-nps) may be found in health and consumer products that routinely contact skin.ObjectivesThis study was designed to assess the potential cytotoxicity of Ag-nps in human epidermal keratinocytes (HEKs) and their inflammatory and penetrating potential into porcine skin in vivo.Materials and MethodsWe used eight different Ag-nps in this study [unwashed/uncoated (20, 50, and 80 nm particle diameter), washed/uncoated (20, 50, and 80 nm), and carbon-coated (25 and 35 nm)]. Skin was dosed topically for 14 consecutive days. HEK viability was assessed by MTT, alamarBlue (aB), and CellTiter 96 AQueous One (96AQ). Release of the proinflammatory mediators interleukin (IL)-1β, IL-6, IL-8, IL-10, and tumor necrosis factor-α (TNF-α) were measured.ResultsThe effect of the unwashed Ag-nps on HEK viability after a 24-hr exposure indicated a significant dose-dependent decrease (p < 0.05) at 0.34 μg/mL with aB and 96AQ and at 1.7 μg/mL with MTT. However, both the washed Ag-nps and carbon-coated Ag-nps showed no significant decrease in viability at any concentration assessed by any of the three assays. For each of the unwashed Ag-nps, we noted a significant increase (p < 0.05) in IL-1β, IL-6, IL-8, and TNF-α concentrations. We observed localization of all Ag-nps in cytoplasmic vacuoles of HEKs. Macroscopic observations showed no gross irritation in porcine skin, whereas microscopic and ultrastructural observations showed areas of focal inflammation and localization of Ag-nps on the surface and in the upper stratum corneum layers of the skin.ConclusionThis study provides a better understanding Ag-nps safety in vitro as well as in vivo and a basis for occupational and risk assessment. Ag-nps are nontoxic when dosed in washed Ag-nps solutions or carbon coated.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro

Samberg

Oldenburg

Monteiro‐Riviere

2010

Environ Health Perspect

450

263

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“…Once excessive ROS production outstrips the anti-oxidative capacity of the cell, oxidative stress is induced with subsequent production of inflammatory mediators, DNA damage and apoptosis [111]. Dermal penetration studies are ideally carried out on porcine skin due to its resemblance to that of humans in terms of thickness and rate of absorption [112]. Samberg et al demonstrated porcine dermal biocompatibility after daily topical application of AgNP-containing cream over a period of 14 consecutive days (0.34–34 μg/mL).…”

Section: Silver Nanoparticlesmentioning

confidence: 99%

Toxicology and clinical potential of nanoparticles

et al. 2011

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“…Hence, various animal models have been suggested as a substitute for human skin, though the drug penetration through animal skin may differ from that in human tissue [16]. It has been reported that percutaneous absorption in monkey [17] and in pig [18, 19] may closely predict that in human. However, such large animals are not readily available and are more expensive than smaller laboratory animals.…”

Section: Discussionmentioning

confidence: 99%

A Novel Murine Model for theIn VivoStudy of Transdermal Drug Penetration

Erős

Hartmann

Berkó

et al. 2012

The Scientific World Journal

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Enhancement of the transdermal penetration of different active agents is an important research goal. Our aim was to establish a novel in vivo experimental model which provides a possibility for exact measurement of the quantity of penetrated drug. The experiments were performed on SKH-1 hairless mice. A skin fold in the dorsal region was fixed with two fenestrated titanium plates. A circular wound was made on one side of the skin fold. A metal cylinder with phosphate buffer was fixed into the window of the titanium plate. The concentration of penetrated drug was measured in the buffer. The skin fold was morphologically intact and had a healthy microcirculation. The drug appeared in the acceptor buffer after 30 min, and its concentration exhibited a continuous increase. The presence of ibuprofen was also detected in the plasma. In conclusion, this model allows an exact in vivo study of drug penetration and absorption.

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Evaluation of animal models for predicting skin penetration in man*1

Cited by 66 publications

References 6 publications

Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro

Evaluation of Silver Nanoparticle Toxicity in Skin in Vivo and Keratinocytes in Vitro

Toxicology and clinical potential of nanoparticles

A Novel Murine Model for theIn VivoStudy of Transdermal Drug Penetration

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